Journal ArticleDOI
Absorption and fluorescence properties of fluorescein
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TLDR
In this article, the authors characterized the protolytic equilibria of fluorescein and determined the spectroscopic properties of its proclivity to fluorescence, and derived the equilibrium constants relating the chemical activities of the cation, neutral form, anion and dianion.About:
This article is published in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.The article was published on 1995-06-01. It has received 951 citations till now. The article focuses on the topics: Quantum yield.read more
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pH-Dependence of the Absorption and Fluorescent Properties of Fluorone Dyes in Aqueous Solutions
TL;DR: A series of fluorone dyes (fluorescein, eosin Y, erythrosin B) in aqueous solution is investigated by the absorption, fluorescence spectroscopic and time-resolved methods.
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A Parallel Multiharmonic Frequency-Domain Fluorometer for Measuring Excited-State Decay Kinetics Following One-, Two-, or Three-Photon Excitation
TL;DR: A new, multiharmonic frequency-domain instrument that uses the high harmonic content of a passively mode-locked, pulse-picked femto-second Ti-sapphire laser as the excitation source for the determination of one-, two-, or three-photon excited time-resolved fluorescence anisotropy and intensity decay kinetics is reported on.
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Spectrophotometric study of the effects of surfactants and ethanol on the acidity constants of fluorescein.
TL;DR: The effects of sodium dodecyl sulfate and Triton X-100 as a surface-active agent on the acidic and basic forms, and the spectral properties of fluorescein were studied by the spectrophotometric method.
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Imaging Water-Splitting Electrocatalysts with pH-Sensing Confocal Fluorescence Microscopy
TL;DR: In this paper, the authors used laser-scanning confocal fluorescence microscopy to map the product/reactant concentration at a H2-evolving electrode at micron-scale resolution which identifies areas with fast reaction kinetics.
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Method for rapid multidiameter single-fiber reflectance and fluorescence spectroscopy through a fiber bundle
Christopher L. Hoy,Ute A. Gamm,Henricus J. C. M. Sterenborg,Dominic J. Robinson,Arjen Amelink +4 more
TL;DR: The construction and validation of a clinical MDSFR/SFF spectroscopy system that avoids the limitations encountered with pinholes and free-space optics is described.
References
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Journal ArticleDOI
Fluorescence detection in automated DNA sequence analysis
Lloyd M. Smith,Jane Z. Sanders,Robert J. Kaiser,Peter Hughes,Chris Dodd,Charles R. Connell,Cheryl Heiner,Stephen B. H. Kent,Leroy Hood +8 more
TL;DR: A method for the partial automation of DNA sequence analysis by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymaticDNA sequence analysis.
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Determination of the absolute quantum yield of fluorescent solutions
Gregorio Weber,F. W. J. Teale +1 more
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Subattomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence
TL;DR: The alanine detection limit corresponds to fewer than 6000 molecules injected onto the column and represents an improvement of four orders of magnitude in the state of the art for fluorescent detection of amino acids and an improvement for the detection limit for isothiocyanate derivatives of amino amino acids.