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Journal ArticleDOI

Aerobic methane production by planktonic microbes in lakes

TLDR
In this paper, a seasonal survey of 14 Japanese freshwater lakes showed that the formation of subsurface methane maximum (SMM) during the summer stratification period is a common feature in large and deep oligotrophic lakes and the development of SMM was closely associated with the seasonal dynamics of planktonic cyanobacteria such as Synechococcus.
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This article is published in Science of The Total Environment.The article was published on 2019-12-15. It has received 25 citations till now. The article focuses on the topics: Aerobic methane production & Atmospheric methane.

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Citations
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Dissolved CH 4 coupled to photosynthetic picoeukaryotes in oxic waters and to cumulative chlorophyll a in anoxic waters of reservoirs

TL;DR: In this article, the authors determined the dissolved CH4 concentration in the water column of 12 reservoirs during summer stratification and winter mixing to explore the role of photosynthetic picoeukaryotes in the CH4 sources in oxic waters.
Journal ArticleDOI

Potential role of submerged macrophytes for oxic methane production in aquatic ecosystems

TL;DR: In this paper , the authors argue that macroalgae and submerged macrophytes may directly or indirectly contribute to CH4 formation in oxic waters and propose several potential direct and indirect mechanisms: (1) direct production of CH4; (2) production of ch4 precursors and facilitation of their bacterial breakdown or chemical conversion; (3) Facilitation of classic methanogenesis; and (4) facilitationof CH4 ebullition.
References
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Naïve Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy

TL;DR: The RDP Classifier can rapidly and accurately classify bacterial 16S rRNA sequences into the new higher-order taxonomy proposed in Bergey's Taxonomic Outline of the Prokaryotes, and the majority of the classification errors appear to be due to anomalies in the current taxonomies.

16S/23S rRNA sequencing

D. J. Lane
Journal ArticleDOI

Relationship between wind speed and gas exchange over the ocean

TL;DR: In this paper, the influence of variability in wind speed on the calculated gas transfer velocities and the possibility of chemical enhancement of CO2 exchange at low wind speeds over the ocean is illustrated using a quadratic dependence of gas exchange on wind speed.
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Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.

TL;DR: Fluorescent oligonucleotide hybridization probes were used to label bacterial cells for analysis by flow cytometry and the intensity of fluorescence was increased additively by the combined use of two or three fluorescent probes complementary to different regions of the same 16S rRNA.
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