Journal ArticleDOI
Affinity chromatography as a tool for antibody purification.
TLDR
The approaches used for antibody purification are critically examined with the aim of providing the reader with the principles and practical insights required to understand the intricacies of the procedures.About:
This article is published in Methods.The article was published on 2012-02-01. It has received 161 citations till now. The article focuses on the topics: Affinity chromatography.read more
Citations
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Journal ArticleDOI
Biosensor Regeneration: A Review of Common Techniques and Outcomes
TL;DR: The merits and drawbacks of regeneration schemes that have been proven in various biosensor systems are discussed and parameters for successful regeneration are indicated based on a systematic review of the literature.
Journal ArticleDOI
Partitioning in aqueous two‐phase systems: Analysis of strengths, weaknesses, opportunities and threats
TL;DR: It can be concluded that most of the perceived weakness towards industrial implementation have now been largely overcome, thus paving the way for opportunities in fermentation feed clarification, integration in multi‐stage operations and in single‐step purification processes.
Journal ArticleDOI
Proteomics-based methods for discovery, quantification, and validation of protein-protein interactions.
TL;DR: An understanding of protein functions cannot be fully accomplished without knowledge of its interactions, and characterizing these interactions is critical to understanding the biology of health and disease systems.
Journal ArticleDOI
Microbial Enzyme Production Using Lignocellulosic Food Industry Wastes as Feedstock: A Review
Rajeev Ravindran,Amit K. Jaiswal +1 more
TL;DR: An overview of the current complications encountered in enzyme production and how accumulating food processing waste can emerge as an environment-friendly and economically feasible solution for a choice of raw material is given.
Journal ArticleDOI
Recent advances on protein separation and purification methods.
TL;DR: The progress of protein separation technology was reviewed from the aspects of extraction, precipitation, membrane separation, chromatography, electrophoresis, molecular imprinting, microfluidic chip and so on.
References
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Journal ArticleDOI
Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of protein A from Staphylococcus aureus at 2.9- and 2.8-A resolution.
TL;DR: The model of human Fc fragment was refined at 2.9-A resolution and the R value of the model is 0.24, with strong arguments that contact 1 is the fragment B-Fc contact formed in solution under physiological conditions, while contact 2 is a crystal contact.
Journal ArticleDOI
Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems.
TL;DR: An overview of the most frequently used and interesting recombinant hybrids containing a polypeptide fusion partner, termed affinity tag, to facilitate the purification of the targetpolypeptides are given.
Book ChapterDOI
[31] Affinity chromatography
TL;DR: Inherent advantages of this method of purification are the rapidity and ease of a potentially single-step procedure, the rapid separation of the protein to be purified from inhibitors and destructive contaminants, such as proteases, and protection from denaturation during purification by active site ligand-stabilization of protein tertiary structure.
Journal ArticleDOI
Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains
Hennie R. Hoogenboom,Andrew D. Griffiths,Kevin Stuart Johnson,David John Chiswell,Peter J. Hudson,Greg Winter +5 more
TL;DR: Heterodimeric Fab fragments can be assembled on the surface of the phages by linking one chain to the phage coat protein, and secreting the other into the bacterial periplasm by introducing an amber mutation.
Journal ArticleDOI
Potent antibody therapeutics by design
TL;DR: The generation of potent antibody therapeutics, which I review here, is an iterative design process that involves the generation and optimization of antibodies to improve their clinical potential.
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