Journal ArticleDOI
Aminoglycoside antibiotics induce bacterial biofilm formation
Lucas R. Hoffman,David A. D'Argenio,Michael J. MacCoss,Zhaoying Zhang,Roger A. Jones,Samuel I. Miller +5 more
TLDR
It is shown that subinhibitory concentrations of aminoglycoside antibiotics induce biofilm formation in P. aeruginosa and Escherichia coli, and the molecular basis of this response includes alterations in the level of c-di-GMP.Abstract:
Biofilms are adherent aggregates of bacterial cells that form on biotic and abiotic surfaces, including human tissues. Biofilms resist antibiotic treatment and contribute to bacterial persistence in chronic infections. Hence, the elucidation of the mechanisms by which biofilms are formed may assist in the treatment of chronic infections, such as Pseudomonas aeruginosa in the airways of patients with cystic fibrosis. Here we show that subinhibitory concentrations of aminoglycoside antibiotics induce biofilm formation in P. aeruginosa and Escherichia coli. In P. aeruginosa, a gene, which we designated aminoglycoside response regulator (arr), was essential for this induction and contributed to biofilm-specific aminoglycoside resistance. The arr gene is predicted to encode an inner-membrane phosphodiesterase whose substrate is cyclic di-guanosine monophosphate (c-di-GMP)-a bacterial second messenger that regulates cell surface adhesiveness. We found that membranes from arr mutants had diminished c-di-GMP phosphodiesterase activity, and P. aeruginosa cells with a mutation changing a predicted catalytic residue of Arr were defective in their biofilm response to tobramycin. Furthermore, tobramycin-inducible biofilm formation was inhibited by exogenous GTP, which is known to inhibit c-di-GMP phosphodiesterase activity. Our results demonstrate that biofilm formation can be a specific, defensive reaction to the presence of antibiotics, and indicate that the molecular basis of this response includes alterations in the level of c-di-GMP.read more
Citations
More filters
Journal ArticleDOI
Cyclic di-GMP: the First 25 Years of a Universal Bacterial Second Messenger
TL;DR: A historic perspective on the development of the field is provided, common trends are emphasized, and new directions in c-di-GMP research are highlighted that will give a deeper understanding of this truly universal bacterial second messenger.
Journal ArticleDOI
Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients.
Eric E. Smith,Danielle Buckley,Zaining Wu,Channakhone Saenphimmachak,Lucas R. Hoffman,David A. D'Argenio,Samuel I. Miller,Bonnie W. Ramsey,David P. Speert,Samuel M. Moskowitz,Jane L. Burns,Rajinder Kaul,Maynard V. Olson +12 more
TL;DR: It is found that virulence factors that are required for the initiation of acute infections are often selected against during chronic infections, including cystic fibrosis patient's P. aeruginosa strains.
Journal ArticleDOI
Microbiological effects of sublethal levels of antibiotics
Dan I. Andersson,Diarmaid Hughes +1 more
TL;DR: The ecology of antibiotics and the ability of subinhibitory concentrations to select for bacterial resistance are discussed and the effects of low-level drug exposure on bacterial physiology are considered, including the generation of genetic and phenotypic variability, as well as the able of antibiotics to function as signalling molecules.
Journal ArticleDOI
Taxonomy, Physiology, and Natural Products of Actinobacteria
Essaid Ait Barka,Parul Vatsa,Lisa Sanchez,Nathalie Gaveau-Vaillant,Cédric Jacquard,Hans-Peter Klenk,Christophe Clément,Yder Ouhdouch,Gilles P. van Wezel +8 more
TL;DR: Actinobacteria are Gram-positive bacteria with high G+C DNA content that constitute one of the largest bacterial phyla, and they are ubiquitously distributed in both aquatic and terrestrial ecosystems.
Journal ArticleDOI
A review of the biomaterials technologies for infection-resistant surfaces.
TL;DR: Among the new strategies, the use of phages or of antisense peptide nucleic acids are discussed, as well as the possibility to modulate the local immune response by active cytokines, to contrast the establishment of an implant infection.
References
More filters
Book
Bergey's Manual of Systematic Bacteriology
TL;DR: BCL3 and Sheehy cite Bergey's manual of determinative bacteriology of which systematic bacteriology, first edition, is an expansion.
Journal ArticleDOI
Goodman and Gilman's the Pharmacological Basis of Therapeutics
William H. Stigelman,Pharm D. +1 more
Journal ArticleDOI
Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development
George A. O'Toole,Roberto Kolter +1 more
TL;DR: The isolation and characterization of mutants of Pseudomonas aeruginosa PA14 defective in the initiation of biofilm formation on an abiotic surface, polyvinylchloride (PVC) plastic are reported and evidence that microcolonies form by aggregation of cells present in the monolayer is presented.
Journal ArticleDOI
Intermittent Administration of Inhaled Tobramycin in Patients with Cystic Fibrosis
Bonnie W. Ramsey,Margaret Pepe,J M Quan,Kelly L. Otto,A. B. Montgomery,Judy Williams-Warren,M. Vasiljev-k,Drucy Borowitz,C M Bowman,B C Marshall,S. G. Marshall,Arnold L. Smith +11 more
TL;DR: In a 24-week study of patients with cystic fibrosis, intermittent administration of inhaled tobramycin was well tolerated and improved pulmonary function, decreased the density of P. aeruginosa in sputum, and decreased the risk of hospitalization.
Journal ArticleDOI
Gene expression in Pseudomonas aeruginosa biofilms
Marvin Whiteley,Gita Bangera,Roger E. Bumgarner,Matthew R. Parsek,Gail M. Teitzel,Stephen Lory,E. P. Greenberg +6 more
TL;DR: DNA microarrays show that gene expression in biofilm cells is similar to that in free-living cells but there are a small number of significant differences, which points to mechanisms of biofilm resistance to antibiotics.