Book ChapterDOI
Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics
Thomas J. White
- Vol. 18, pp 315-322
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The article was published on 1990-01-01. It has received 26241 citations till now. The article focuses on the topics: 18S ribosomal RNA & 28S ribosomal RNA.read more
Citations
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Journal ArticleDOI
RNA reveals a succession of active fungi during the decay of Norway spruce logs
TL;DR: This work investigated the changing community of metabolically active fungi during the decomposition of fallen Picea abies logs by directly extracting and barcode sequencing precursor rRNA and compared rRNA-derived amplicons of the 18S and ITS regions in 21 isolates.
Journal ArticleDOI
Taxonomy and phylogeny of the xerophilic genus Wallemia (Wallemiomycetes and Wallemiales, cl. et ord. nov.)
TL;DR: Tests on xerotolerance indicated that Wallemia presents one of the most xerophilic fungal taxa, and a new basidiomycetous class Wallemiomycets covering an order Wallemiales is proposed.
Journal ArticleDOI
Rapid Identification of Candida Species with Species-Specific DNA Probes
TL;DR: This method is simple, rapid, and feasible for identifying Candida species in clinical laboratories that utilize molecular identification techniques and provides a novel method to differentiate the new species, C. dubliniensis, from C. albicans.
Journal ArticleDOI
Six monophyletic lineages identified within Cryptococcus neoformans and Cryptococcus gattii by multi-locus sequence typing.
TL;DR: Four monophyletic lineages corresponding to the previously described genotypic groups were consistently found within C. gattii, indicating that these lineages should be considered different taxa as well.
Journal ArticleDOI
Identification and characterization of benzimidazole resistance in Monilinia fructicola from stone fruit orchards in California
TL;DR: Low and high levels of resistance to the benzimidazole fungicides benomyl and thiophanate-methyl were observed in field isolates of Monilinia fructicola, which is the causative agent of brown rot of stone fruit.
References
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Journal ArticleDOI
Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses
TL;DR: A protocol is described for rapidly generating large blocks of 16S rRNA sequence data without isolation of the 16 S rRNA or cloning of its gene, and its phylogenetic usefulness is evaluated by examination of several 17S rRNAs whose gene sequences are known.
Journal ArticleDOI
The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions.
TL;DR: Oligodeoxynucleotides that are complementary to conserved regions at the 5' and 3' termini of eukaryotic 16S-like rRNAs were used to prime DNA synthesis in repetitive cycles of denaturation, reannealing, and DNA synthesis.
Journal ArticleDOI
Hybridization of nucleic acids immobilized on solid supports
Judy Meinkoth,Geoffrey M. Wahl +1 more
Journal ArticleDOI
Compilation of small ribosomal subunit RNA sequences
TL;DR: The set of 452 different sequences comprises all sequences that to the authors' knowledge had been published or were available from the sequence library file servers as of December 1, 1990, and that are either complete or cover a minimum of about 70% of the complete sequence.
Journal ArticleDOI
Archaebacterial phylogeny: Perspectives on the Urkingdoms*
TL;DR: By 16S rRNA sequence measure the archaebacteria constitute a phylogenetically coherent grouping (clade), which excludes both the eubacteria and the eukaryotes--a conclusion that is supported by other sequence evidence as well.