Book ChapterDOI
Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics
Thomas J. White
- Vol. 18, pp 315-322
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The article was published on 1990-01-01. It has received 26241 citations till now. The article focuses on the topics: 18S ribosomal RNA & 28S ribosomal RNA.read more
Citations
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Journal ArticleDOI
Increased efficiency in identifying mixed pollen samples by meta-barcoding with a dual-indexing approach
Wiebke Sickel,Markus J. Ankenbrand,Gudrun Grimmer,Andrea Holzschuh,Stephan Härtel,Jonathan Lanzen,Ingolf Steffan-Dewenter,Alexander Keller +7 more
TL;DR: A new primer-adapter design for high throughput sequencing with the Illumina technology that uses a dual-indexing strategy, where sample-specific combinations of forward and reverse identifiers attached to the barcode marker allow high sample throughput with a single sequencing run.
Journal ArticleDOI
Fungal epiphytes and endophytes of coffee leaves (Coffea arabica).
Johanna Santamaría,Paul Bayman +1 more
TL;DR: This first quantitative comparison of epiphytic and endophytic fungal floras in any plant is compared, and significant correlations between occurrence of fungal morphospecies suggested that fungi may have positive or negative effects on their neighbors.
Journal ArticleDOI
Effect of inoculation with phosphate solubilizing fungus on growth and nutrient uptake of wheat and maize plants fertilized with rock phosphate in alkaline soils
Himani Singh,M. Sudhakara Reddy +1 more
TL;DR: In this paper, the fungus Penicillium oxalicum was isolated from the rhizosphere soil of rock phosphate mine landfills and tested for its efficacy to solubilize rock phosphate (RP) as well as promotion of the growth of wheat and maize plants grown in soil amended with RP.
Journal ArticleDOI
Molecular phylogenetics of the Chytridiomycota supports the utility of ultrastructural data in chytrid systematics
TL;DR: A phylogeny of ribosomal DNA sequences coding for the small subunit gene of 54 chytrids, with emphasis on sampling the largest order, the Chytridiales, revealed homoplasy in several developmental and zoosporangial characters and suggested that the divergence times of these groups may be ancient.
Journal ArticleDOI
A global meta-analysis of Tuber ITS rDNA sequences: species diversity, host associations and long-distance dispersal.
TL;DR: It is estimated that Tuber contains a minimum of 180 species and species in Excavatum, Maculatum and Rufum clades exhibit preference for angiosperm hosts, whereas those in the Gibbosum clade are preferential towards gymnosperms.
References
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Journal ArticleDOI
Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses
TL;DR: A protocol is described for rapidly generating large blocks of 16S rRNA sequence data without isolation of the 16 S rRNA or cloning of its gene, and its phylogenetic usefulness is evaluated by examination of several 17S rRNAs whose gene sequences are known.
Journal ArticleDOI
The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions.
TL;DR: Oligodeoxynucleotides that are complementary to conserved regions at the 5' and 3' termini of eukaryotic 16S-like rRNAs were used to prime DNA synthesis in repetitive cycles of denaturation, reannealing, and DNA synthesis.
Journal ArticleDOI
Hybridization of nucleic acids immobilized on solid supports
Judy Meinkoth,Geoffrey M. Wahl +1 more
Journal ArticleDOI
Compilation of small ribosomal subunit RNA sequences
TL;DR: The set of 452 different sequences comprises all sequences that to the authors' knowledge had been published or were available from the sequence library file servers as of December 1, 1990, and that are either complete or cover a minimum of about 70% of the complete sequence.
Journal ArticleDOI
Archaebacterial phylogeny: Perspectives on the Urkingdoms*
TL;DR: By 16S rRNA sequence measure the archaebacteria constitute a phylogenetically coherent grouping (clade), which excludes both the eubacteria and the eukaryotes--a conclusion that is supported by other sequence evidence as well.