Book ChapterDOI
Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics
Thomas J. White
- Vol. 18, pp 315-322
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The article was published on 1990-01-01. It has received 26241 citations till now. The article focuses on the topics: 18S ribosomal RNA & 28S ribosomal RNA.read more
Citations
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Journal ArticleDOI
Molecular phylogeny of northern hemisphere species of Armillaria
TL;DR: This study used polymerase chain reaction amplification and direct sequencing of double-stranded DNA to compare the intergenic region between the 3' end of the 26S gene and the 5S gene of the nuclear, ribosomal DNA of several northern hemisphere species of Armillaria.
Journal ArticleDOI
Redefinition of Aureobasidium Pullulans and Its Varieties
TL;DR: To characterise the genetic variability of Aureobasidium pullulans strains originating from the Arctic and strains originating pan-globally, a multilocus molecular analysis was performed and a partial elongase-encoding gene was successfully used as a phylogenetic marker at the (infra-)specific level.
Journal ArticleDOI
Mycorrhizal diversity in photosynthetic terrestrial orchids
TL;DR: The genetic diversity of mycorrhizal fungi associated with an evergreen, a spring-green, and a winter-green orchid and compared this diversity with that published for a nonphotosynthetic orchid was compared.
Journal ArticleDOI
Inter‐ and intraspecific variation in the ITS region of rDNA of ectomycorrhizal fungi in Fennoscandia as detected by endonuclease analysis
TL;DR: It is concluded that most ectomycorrhizas formed by the 44 investigated species should be recognized by comparison with this dataset, if the mycorrhIZas are sampled from a site located in Fennoscandia, but in datasets from even larger geographical areas encompassing a higher degree of intraspecific variation in the ITS region, it might be necessary to include local reference species.
Journal ArticleDOI
Environmental stress destabilizes microbial networks.
Damian J. Hernandez,Aaron S. David,Aaron S. David,Eric S. Menges,Christopher A. Searcy,Michelle E. Afkhami +5 more
TL;DR: In this paper, the authors present the first evidence that naturally-occurring microbiomes display network properties characteristic of unstable communities when under persistent stress, showing that prokaryotic and fungal diversity decline in high stress, and negative:positive cohesion have a clear negative relationship with environmental stress.
References
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Journal ArticleDOI
Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses
TL;DR: A protocol is described for rapidly generating large blocks of 16S rRNA sequence data without isolation of the 16 S rRNA or cloning of its gene, and its phylogenetic usefulness is evaluated by examination of several 17S rRNAs whose gene sequences are known.
Journal ArticleDOI
The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions.
TL;DR: Oligodeoxynucleotides that are complementary to conserved regions at the 5' and 3' termini of eukaryotic 16S-like rRNAs were used to prime DNA synthesis in repetitive cycles of denaturation, reannealing, and DNA synthesis.
Journal ArticleDOI
Hybridization of nucleic acids immobilized on solid supports
Judy Meinkoth,Geoffrey M. Wahl +1 more
Journal ArticleDOI
Compilation of small ribosomal subunit RNA sequences
TL;DR: The set of 452 different sequences comprises all sequences that to the authors' knowledge had been published or were available from the sequence library file servers as of December 1, 1990, and that are either complete or cover a minimum of about 70% of the complete sequence.
Journal ArticleDOI
Archaebacterial phylogeny: Perspectives on the Urkingdoms*
TL;DR: By 16S rRNA sequence measure the archaebacteria constitute a phylogenetically coherent grouping (clade), which excludes both the eubacteria and the eukaryotes--a conclusion that is supported by other sequence evidence as well.