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Book ChapterDOI

Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics

Thomas J. White
- Vol. 18, pp 315-322
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The article was published on 1990-01-01. It has received 26241 citations till now. The article focuses on the topics: 18S ribosomal RNA & 28S ribosomal RNA.

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Journal ArticleDOI

Rich and cold: diversity, distribution and drivers of fungal communities in patterned-ground ecosystems of the North American Arctic.

TL;DR: The results suggest that the Arctic does not host a unique mycoflora, while Arctic fungi are highly sensitive to climate and vegetation, with potential to migrate rapidly as global change unfolds.
Journal ArticleDOI

Fossil mushrooms from miocene and cretaceous ambers and the evolution of homobasidiomycetes

TL;DR: Two species of fossil mushrooms that are similar to extant Tricholomataceae are described from Cretaceous and Miocene ambers and suggests that in certain lineages homobasidiomycete macroevolution has also involved long periods during which there has been little morphological change.
Journal ArticleDOI

Internal Transcribed Spacer Sequencing versus Biochemical Profiling for Identification of Medically Important Yeasts

TL;DR: An in-house database of yeast internal transcribed spacer (ITS) sequences is established and a three-step procedure which integrates morphological criteria, biochemical investigation, and sequence analysis of the ITS region is suggested.
Journal ArticleDOI

Global diversity of the Ganoderma lucidum complex (Ganodermataceae, Polyporales) inferred from morphology and multilocus phylogeny.

TL;DR: 32 collections of the Ganoderma lucidum complex from Asia, Europe and North America were analyzed from both morphological and molecular phylogenetic perspectives, and a total of 13 species of the complex were recovered in the multilocus phylogeny.
Journal ArticleDOI

Vertical Transmission of Endobacteria in the Arbuscular Mycorrhizal Fungus Gigaspora margarita through Generation of Vegetative Spores

TL;DR: The study demonstrates that a vertical transmission of endobacteria takes place through the fungal vegetative generations (sporulation) of an AM fungus, indicating that active bacterial proliferation occurs in the coenocytic mycelium of the fungus, and suggests that these bacteria are obligate endocellular components of their AM fungal host.
References
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Journal ArticleDOI

Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses

TL;DR: A protocol is described for rapidly generating large blocks of 16S rRNA sequence data without isolation of the 16 S rRNA or cloning of its gene, and its phylogenetic usefulness is evaluated by examination of several 17S rRNAs whose gene sequences are known.
Journal ArticleDOI

The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions.

TL;DR: Oligodeoxynucleotides that are complementary to conserved regions at the 5' and 3' termini of eukaryotic 16S-like rRNAs were used to prime DNA synthesis in repetitive cycles of denaturation, reannealing, and DNA synthesis.
Journal ArticleDOI

Compilation of small ribosomal subunit RNA sequences

TL;DR: The set of 452 different sequences comprises all sequences that to the authors' knowledge had been published or were available from the sequence library file servers as of December 1, 1990, and that are either complete or cover a minimum of about 70% of the complete sequence.
Journal ArticleDOI

Archaebacterial phylogeny: Perspectives on the Urkingdoms*

TL;DR: By 16S rRNA sequence measure the archaebacteria constitute a phylogenetically coherent grouping (clade), which excludes both the eubacteria and the eukaryotes--a conclusion that is supported by other sequence evidence as well.
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