Book ChapterDOI
Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics
Thomas J. White
- Vol. 18, pp 315-322
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The article was published on 1990-01-01. It has received 26241 citations till now. The article focuses on the topics: 18S ribosomal RNA & 28S ribosomal RNA.read more
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Journal ArticleDOI
Fungal endophyte diversity in coffee plants from Colombia, Hawai'i, Mexico and Puerto Rico
Fernando E. Vega,Ann Simpkins,M. Catherine Aime,Francisco Posada,Stephen W. Peterson,Stephen A. Rehner,Francisco Infante,Alfredo Castillo,A. Elizabeth Arnold +8 more
TL;DR: This study demonstrates that coffee plants serve as a reservoir for a wide variety of fungal endophytes that can be isolated from various plant tissues, including the seed, and illustrates the different fungal communities encountered by C. arabica in different coffee-growing regions of the world.
Journal ArticleDOI
Dynamics of indigenous yeast populations during spontaneous fermentation of wines from Mendoza, Argentina.
Mariana Combina,Mariana Combina,A. Elia,Laura Analia Mercado,Laura Analia Mercado,C. Catania,Angélica Ganga,Claudio Martínez +7 more
TL;DR: This work shows the significant participation of non-Saccharomyces yeasts during spontaneous fermentation of musts, with the ubiquitous presence of three main species: Kloeckera apiculata, Candida stellata and Metschnikowia pulcherrima.
Journal ArticleDOI
PCR Amplification of Ribosomal DNA for Species Identification in the Plant Pathogen Genus Phytophthora
TL;DR: PCR was superior to traditional isolation methods for detection of P. capsici in infected bell pepper tissue in field samples and will provide a powerful tool for identification of the major species in the genusPhytophthora.
Journal ArticleDOI
Diversity of fungi in organic soils under a moorland--Scots pine (Pinus sylvestris L.) gradient.
TL;DR: The consequences of regeneration on terrestrial fungal communities was determined in three parallel transects running from open moorland, through an intermediate zone showing seedling colonization, into a mature Scots pine forest at Abernethy Forest, Cairngorm, Scotland.
Journal ArticleDOI
Pan-European distribution of white-nose syndrome fungus (Geomyces destructans) not associated with mass mortality.
Sébastien J. Puechmaille,Gudrun Wibbelt,Vanessa Korn,Hubert T. Fuller,Frédéric Forget,Kristin Mühldorfer,Andreas Kurth,Wiesław Bogdanowicz,Christophe Borel,Thijs Bosch,Thomas Cherezy,Mikhail Drebet,Tamás Görföl,Anne-Jifke Haarsma,Frank Herhaus,Guénael Hallart,Matthias Hammer,Christian Jungmann,Yann Le Bris,Lauri Lutsar,Matti Masing,Bart Mulkens,Karsten Passior,Martin Starrach,Andrzej Wojtaszewski,Ulrich Zöphel,Emma C. Teeling +26 more
TL;DR: The characterisation of the temporal variation in G. destructans growth on bats provides reference data for studying the spatio-temporal dynamic of the fungus, and the presence of G. destructionans spores on cave walls suggests that hibernacula could act as passive vectors and/or reservoirs for G.destructans and therefore, might play an important role in the transmission process.
References
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Journal ArticleDOI
Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses
TL;DR: A protocol is described for rapidly generating large blocks of 16S rRNA sequence data without isolation of the 16 S rRNA or cloning of its gene, and its phylogenetic usefulness is evaluated by examination of several 17S rRNAs whose gene sequences are known.
Journal ArticleDOI
The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions.
TL;DR: Oligodeoxynucleotides that are complementary to conserved regions at the 5' and 3' termini of eukaryotic 16S-like rRNAs were used to prime DNA synthesis in repetitive cycles of denaturation, reannealing, and DNA synthesis.
Journal ArticleDOI
Hybridization of nucleic acids immobilized on solid supports
Judy Meinkoth,Geoffrey M. Wahl +1 more
Journal ArticleDOI
Compilation of small ribosomal subunit RNA sequences
TL;DR: The set of 452 different sequences comprises all sequences that to the authors' knowledge had been published or were available from the sequence library file servers as of December 1, 1990, and that are either complete or cover a minimum of about 70% of the complete sequence.
Journal ArticleDOI
Archaebacterial phylogeny: Perspectives on the Urkingdoms*
TL;DR: By 16S rRNA sequence measure the archaebacteria constitute a phylogenetically coherent grouping (clade), which excludes both the eubacteria and the eukaryotes--a conclusion that is supported by other sequence evidence as well.