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Book ChapterDOI

Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics

Thomas J. White
- Vol. 18, pp 315-322
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The article was published on 1990-01-01. It has received 26241 citations till now. The article focuses on the topics: 18S ribosomal RNA & 28S ribosomal RNA.

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Journal ArticleDOI

Taxonomy, ecology and biotechnological applications of thraustochytrids: A review

TL;DR: The taxonomic history of thraustochytrids is reviewed, while providing an up-to-date classification of these organisms and the various biomarkers that may be taken into consideration to support taxonomic characterization are described.
Journal ArticleDOI

Yeasts isolated from three varieties of grapes cultivated in different locations of the Dolenjska vine-growing region, Slovenia.

TL;DR: The aim of this study was to evaluate the impact of geographical locations in the Dolenjska vine-growing region and to obtain precise information about the influence of different grape varieties on the composition of yeast community on grape berries.
Journal ArticleDOI

Effect of monoculture soybean on soil microbial community in the Northeast China

TL;DR: DGGE patterns showed that DGGE patterns were not able to detect significant differences in diversity or evenness among microbial communities, but significant differences were found in the composition of bacterial and fungal community structures.
Journal ArticleDOI

PCR and single-strand conformational polymorphism for recognition of medically important opportunistic fungi.

TL;DR: The technique of PCR-SSCP provides a novel method by which to recognize and distinguish medically important opportunistic fungi and which has potential applications to molecular diagnosis, taxonomic classification, molecular epidemiology, and elucidation of mechanisms of antifungal drug resistance.
Journal ArticleDOI

Elevated seawater temperature causes a microbial shift on crustose coralline algae with implications for the recruitment of coral larvae

TL;DR: This is the first experimental evidence to demonstrate how thermal stress influences microbial associations on CCA with subsequent downstream impacts on coral recruitment, which is critical for reef regeneration and recovery from climate-related mortality events.
References
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Journal ArticleDOI

Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses

TL;DR: A protocol is described for rapidly generating large blocks of 16S rRNA sequence data without isolation of the 16 S rRNA or cloning of its gene, and its phylogenetic usefulness is evaluated by examination of several 17S rRNAs whose gene sequences are known.
Journal ArticleDOI

The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions.

TL;DR: Oligodeoxynucleotides that are complementary to conserved regions at the 5' and 3' termini of eukaryotic 16S-like rRNAs were used to prime DNA synthesis in repetitive cycles of denaturation, reannealing, and DNA synthesis.
Journal ArticleDOI

Compilation of small ribosomal subunit RNA sequences

TL;DR: The set of 452 different sequences comprises all sequences that to the authors' knowledge had been published or were available from the sequence library file servers as of December 1, 1990, and that are either complete or cover a minimum of about 70% of the complete sequence.
Journal ArticleDOI

Archaebacterial phylogeny: Perspectives on the Urkingdoms*

TL;DR: By 16S rRNA sequence measure the archaebacteria constitute a phylogenetically coherent grouping (clade), which excludes both the eubacteria and the eukaryotes--a conclusion that is supported by other sequence evidence as well.
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