Book ChapterDOI
Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics
Thomas J. White
- Vol. 18, pp 315-322
Reads0
Chats0
About:
The article was published on 1990-01-01. It has received 26241 citations till now. The article focuses on the topics: 18S ribosomal RNA & 28S ribosomal RNA.read more
Citations
More filters
Journal ArticleDOI
Ecological network analysis reveals the inter-connection between soil biodiversity and ecosystem function as affected by land use across Europe
Rachel Creamer,S.E. Hannula,J. van Leeuwen,D. Stone,D. Stone,Michiel Rutgers,Rüdiger M. Schmelz,P.C. de Ruiter,N.Bohse Hendriksen,Thomas Bolger,Marie-Lara Bouffaud,Marc Buée,Filipe Carvalho,Dalila Costa,Tara Dirilgen,Romeu Francisco,Bryan S. Griffiths,Robert I. Griffiths,Francis Martin,P. Martins da Silva,Sara Mendes,Paula V. Morais,Carla Pereira,Laurent Philippot,Pierre Plassart,Dirk Redecker,Jörg Römbke,José Paulo Sousa,Marja Wouterse,Philippe Lemanceau +29 more
TL;DR: The EcoFINDERS (FP7) project sampled 76 sites across 11 European countries, covering five biogeographical zones (Alpine, Atlantic, Boreal, Continental and Mediterranean) and three land-uses (arable, grass, forestry) to assess the range in biodiversity and ecosystem function across the sites, fourteen biological methods were applied as proxy indicators for these functions.
Journal ArticleDOI
Yeast species associated with wine grapes in China.
TL;DR: This study is the first to identify the yeast communities associated with grapes in China using molecular methods and the results enrich the knowledge of wine-related microorganisms, and can be used to promote the development of the local wine industry.
Journal ArticleDOI
Detection and identification of Phytophthora fragariae Hickman by the polymerase chain reaction
Peter J. M. Bonants,M. Hagenaar-de Weerdt,M.P.E. van Gent-Pelzer,I. Lacourt,David E. L. Cooke,James M. Duncan +5 more
TL;DR: The fungus was readily detected in infected but symptomless roots by nested, but not single-round, PCR and detection of PCR products was at least 10-fold more sensitive by PCR-ELISA than by conventional visualisation on agarose gels.
Journal ArticleDOI
Genetic and Morphological Characterization of Colletotrichum acutatum Causing Anthracnose of Lupins.
TL;DR: ITS, tub2, and his4 results strongly support designating lupin anthracnose pathogen as C. acutatum or its subspecies, and underline the potential of the C. Acutatum-specific PCR for routine pathogen diagnosis.
Journal ArticleDOI
Centaurea Revisited: A Molecular Survey of the Jacea Group
Núria Garcia-Jacas,Tuna Uysal,Kostyantyn Romashchenko,Víctor N. Suárez-Santiago,Kuddisi Ertuğrul,Alfonso Susanna +5 more
TL;DR: The results show that the sectional classification in current use needs major revision, with many old sections being merged into larger ones and most recent groups in the eastern Mediterranean clade are not resolved and reticulation in the western Mediterranean group of sections makes phylogenetic relationships within these two groups somewhat obscure.
References
More filters
Journal ArticleDOI
Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses
TL;DR: A protocol is described for rapidly generating large blocks of 16S rRNA sequence data without isolation of the 16 S rRNA or cloning of its gene, and its phylogenetic usefulness is evaluated by examination of several 17S rRNAs whose gene sequences are known.
Journal ArticleDOI
The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions.
TL;DR: Oligodeoxynucleotides that are complementary to conserved regions at the 5' and 3' termini of eukaryotic 16S-like rRNAs were used to prime DNA synthesis in repetitive cycles of denaturation, reannealing, and DNA synthesis.
Journal ArticleDOI
Hybridization of nucleic acids immobilized on solid supports
Judy Meinkoth,Geoffrey M. Wahl +1 more
Journal ArticleDOI
Compilation of small ribosomal subunit RNA sequences
TL;DR: The set of 452 different sequences comprises all sequences that to the authors' knowledge had been published or were available from the sequence library file servers as of December 1, 1990, and that are either complete or cover a minimum of about 70% of the complete sequence.
Journal ArticleDOI
Archaebacterial phylogeny: Perspectives on the Urkingdoms*
TL;DR: By 16S rRNA sequence measure the archaebacteria constitute a phylogenetically coherent grouping (clade), which excludes both the eubacteria and the eukaryotes--a conclusion that is supported by other sequence evidence as well.