Book ChapterDOI
Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics
Thomas J. White
- Vol. 18, pp 315-322
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The article was published on 1990-01-01. It has received 26241 citations till now. The article focuses on the topics: 18S ribosomal RNA & 28S ribosomal RNA.read more
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Journal ArticleDOI
Multiple hybrid formation in natural populations: Concerted evolution of the internal transcribed spacer of nuclear ribosomal DNA (ITS) in north American Arabis divaricarpa (Brassicaceae)
TL;DR: Analysis of conserved regions and secondary structures of the ITS region provided no evidence that, in this system, hybrid ITS evolution is predominantly driven in a particular direction, however, two regions in the ITS1 and ITS2, respectively, show higher mutation rates than expected from outgroup comparisons.
Journal ArticleDOI
Phylogenetic and morphotaxonomic revision of Ramichloridium and allied genera
TL;DR: The phylogeny of the genera Periconiella, Ramichloridium, Rhinocladiella and Veronaea was explored by means of partial sequences of the 28S (LSU) rRNA gene and the ITS region and a new genus, Radulidium, is erected.
Journal ArticleDOI
Leptographium wingfieldii introduced into North America and found associated with exotic Tomicus piniperda and native bark beetles
Karin Jacobs,D. R. Bergdahl,Michael J. Wingfield,Shari Halik,Keith A. Seifert,Donald E. Bright,Brenda D. Wingfield +6 more
TL;DR: The first record of L. wingfieldii associated with the introduced and damaging pine shoot beetle T. piniperda in North America shows that the fungus is well established and can become associated with other native bark beetles that attack stressed and/or dying trees.
Journal ArticleDOI
Resolving the Phoma enigma
TL;DR: The aim of the present study was to clarify the generic delimitation in Didymellaceae by combing multi-locus phylogenetic analyses based on ITS, LSU, rpb2 and tub2, and morphological observations, and the resulting phylogenetic tree revealed 17 well-supported monophyletic clades.
Journal ArticleDOI
Analysis of yeast populations during alcoholic fermentation: A six year follow-up study
Gemma Beltran,María Jesús Torija,Maite Novo,Noemí Ferrer,M. Poblet,José Manuel Guillamón,Nicolas Rozès,Albert Mas +7 more
TL;DR: The analysis of the S. cerevisiae strains showed that indigenous strains competed with commercial strains inoculated in other fermentation tanks of the cellar, resulting in the continuous use of commercial yeasts reduced the diversity and importance of the indigenous S. continentaliae strains.
References
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Journal ArticleDOI
Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses
TL;DR: A protocol is described for rapidly generating large blocks of 16S rRNA sequence data without isolation of the 16 S rRNA or cloning of its gene, and its phylogenetic usefulness is evaluated by examination of several 17S rRNAs whose gene sequences are known.
Journal ArticleDOI
The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions.
TL;DR: Oligodeoxynucleotides that are complementary to conserved regions at the 5' and 3' termini of eukaryotic 16S-like rRNAs were used to prime DNA synthesis in repetitive cycles of denaturation, reannealing, and DNA synthesis.
Journal ArticleDOI
Hybridization of nucleic acids immobilized on solid supports
Judy Meinkoth,Geoffrey M. Wahl +1 more
Journal ArticleDOI
Compilation of small ribosomal subunit RNA sequences
TL;DR: The set of 452 different sequences comprises all sequences that to the authors' knowledge had been published or were available from the sequence library file servers as of December 1, 1990, and that are either complete or cover a minimum of about 70% of the complete sequence.
Journal ArticleDOI
Archaebacterial phylogeny: Perspectives on the Urkingdoms*
TL;DR: By 16S rRNA sequence measure the archaebacteria constitute a phylogenetically coherent grouping (clade), which excludes both the eubacteria and the eukaryotes--a conclusion that is supported by other sequence evidence as well.