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Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics

Thomas J. White
- Vol. 18, pp 315-322
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The article was published on 1990-01-01. It has received 26241 citations till now. The article focuses on the topics: 18S ribosomal RNA & 28S ribosomal RNA.

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Host and geographic structure of endophytic and endolichenic fungi at a continental scale

TL;DR: This study provides a first evaluation of endophytic and endolichenic fungal associations with their hosts at a continental scale and sets the stage for empirical assessments of ecological specificity, metabolic capability, and comparative genomics.
Journal ArticleDOI

Communities of arbuscular mycorrhizal fungi in arable soils are not necessarily low in diversity

TL;DR: Data show that the diversity of AMF is not always low in arable soils, and suggests that low‐input agriculture involving crop rotation may provide better conditions to preserve AMF diversity, by preventing the selection for the few AMF taxa tolerating high nutrient levels.
Journal ArticleDOI

Ancestral lineages of arbuscular mycorrhizal fungi (Glomales).

TL;DR: Using new and existing 18S rRNA sequence data, it is shown that at least five species of glomalean fungi lie outside the previously defined families and diverged very early in the evolution of that group.
Journal ArticleDOI

Phosphate solubilizing microorganisms isolated from rhizosphere of maize cultivated in an oxisol of the Brazilian Cerrado Biome.

TL;DR: A greater diversity of P-solubilizing microorganisms was observed in the rhizosphere of the P efficient maize genotypes suggesting that the P efficiency in these cultivars may be related to the potential to enhance microbial interactions of P+ microorganisms.
References
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Journal ArticleDOI

Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses

TL;DR: A protocol is described for rapidly generating large blocks of 16S rRNA sequence data without isolation of the 16 S rRNA or cloning of its gene, and its phylogenetic usefulness is evaluated by examination of several 17S rRNAs whose gene sequences are known.
Journal ArticleDOI

The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions.

TL;DR: Oligodeoxynucleotides that are complementary to conserved regions at the 5' and 3' termini of eukaryotic 16S-like rRNAs were used to prime DNA synthesis in repetitive cycles of denaturation, reannealing, and DNA synthesis.
Journal ArticleDOI

Compilation of small ribosomal subunit RNA sequences

TL;DR: The set of 452 different sequences comprises all sequences that to the authors' knowledge had been published or were available from the sequence library file servers as of December 1, 1990, and that are either complete or cover a minimum of about 70% of the complete sequence.
Journal ArticleDOI

Archaebacterial phylogeny: Perspectives on the Urkingdoms*

TL;DR: By 16S rRNA sequence measure the archaebacteria constitute a phylogenetically coherent grouping (clade), which excludes both the eubacteria and the eukaryotes--a conclusion that is supported by other sequence evidence as well.
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