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Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics

Thomas J. White
- Vol. 18, pp 315-322
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The article was published on 1990-01-01. It has received 26241 citations till now. The article focuses on the topics: 18S ribosomal RNA & 28S ribosomal RNA.

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Journal ArticleDOI

Diversity of fungal endophytes in leaves and stems of wild rubber trees (Hevea brasiliensis) in Peru

TL;DR: Comparisons between leaves and sapwood demonstrated a spatial heterogeneity in endophyte assemblages among plant parts and sites, with a greater diversity of endophytes found in sapwood than in leaves.
Book

Mycoremediation: Fungal Bioremediation

TL;DR: This work focuses on the treatment of Fungal Biodegradation and Biodeterioration of Petroleum Hydrocarbons, and the relationships between Hydrocarbon Metabolism by Yeasts and Fungi and Alternative Treatment Technologies.
Journal ArticleDOI

Phylogenetic trait conservatism and the evolution of functional trade-offs in arbuscular mycorrhizal fungi.

TL;DR: Variability in colonization strategies evolved early in the diversification of AM fungi, and it is proposed that these strategies were influenced by functional interactions with host plants, resulting in an evolutionary stasis resembling trait conservatism.
Journal ArticleDOI

Molecular Phylogeny of the Pseudallescheria boydii Species Complex: Proposal of Two New Species

TL;DR: A morphological and molecular study involving numerous strains of clinical or environmental origins and from different countries has demonstrated that P. boydii is a species complex and Pseudallescheria minutispora and Scedosporium aurantiacum are clearly phylogenetically separated from the other species studied and are proposed as new.
Journal ArticleDOI

Identification of Medically Important Yeast Species by Sequence Analysis of the Internal Transcribed Spacer Regions

TL;DR: Identification of medically important yeasts by ITS sequencing, especially using the ITS2 region, is reliable and can be used as an accurate alternative to conventional identification methods.
References
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Journal ArticleDOI

Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses

TL;DR: A protocol is described for rapidly generating large blocks of 16S rRNA sequence data without isolation of the 16 S rRNA or cloning of its gene, and its phylogenetic usefulness is evaluated by examination of several 17S rRNAs whose gene sequences are known.
Journal ArticleDOI

The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions.

TL;DR: Oligodeoxynucleotides that are complementary to conserved regions at the 5' and 3' termini of eukaryotic 16S-like rRNAs were used to prime DNA synthesis in repetitive cycles of denaturation, reannealing, and DNA synthesis.
Journal ArticleDOI

Compilation of small ribosomal subunit RNA sequences

TL;DR: The set of 452 different sequences comprises all sequences that to the authors' knowledge had been published or were available from the sequence library file servers as of December 1, 1990, and that are either complete or cover a minimum of about 70% of the complete sequence.
Journal ArticleDOI

Archaebacterial phylogeny: Perspectives on the Urkingdoms*

TL;DR: By 16S rRNA sequence measure the archaebacteria constitute a phylogenetically coherent grouping (clade), which excludes both the eubacteria and the eukaryotes--a conclusion that is supported by other sequence evidence as well.
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