Journal ArticleDOI
An in vivo evaluation of induction of abnormal sperm morphology in mice by landfill leachates
TLDR
Physico-chemical analysis of the test samples shows that they contained constituents that are capable of inducing mutation in biologic system, which is relevant in environmental waste management, and for the assessment of the hazardous effects of the chemicals in landfill leachates.Abstract:
Although several reports have demonstrated the acutely toxic and genotoxic effects of landfill leachates in microbial organisms, plants and aquatic animals, the effects of pollutants present in these leachates have not been clarified yet in terrestrial animals. This study mainly aimed to evaluate a potential genetic effect of raw and simulated leachates from Orita-Aperin and Oworonsoki landfills in south-west Nigeria by use of the murine sperm-head abnormality test. These landfills neither have a synthetic membrane liner at the bottom, nor a natural layer of compacted soil with the desired hydraulic conductivity, nor a run-off control system. As a result, the leachates produced are discharged into the environment. Samples designated as Orita-Aperin Raw Leachate (OARL), Orita-Aperin Simulated Leachate (OASL), Oworonsoki Raw Leachate (OWRL) and Oworonsoki Simulated Leachate (OWSL) were analyzed in the sperm-head abnormality test at concentrations (v/v) of 1%, 2.5%, 5%, 10% and 25%. Mice were given 0.5 ml sample per day for five consecutive days by intraperitoneal injection. Each dose group comprised seven mice, and a 5-week exposure period was utilized. The data show that the test mixtures induced a dose-dependent, statistically significant increase (Pread more
Citations
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Journal ArticleDOI
Contemporary Environmental Issues of Landfill Leachate: Assessment and Remedies
TL;DR: In this paper, the authors focus on leachate composition, plume migration, and contaminant façade contamination in landfill leachates and its treatment prior to disposal into the environment.
Journal ArticleDOI
Bioassays for toxicological risk assessment of landfill leachate: A review
TL;DR: From the review it appears that there is a need for a multispecies approach to evaluate leachate toxicity, and different bioassays available for assessing the hazard posed by landfill leachates are reviewed.
Journal ArticleDOI
Analysis of the contaminants released from municipal solid waste landfill site: A case study.
TL;DR: The statistical analysis and spatial variations indicated the leaching of metals from the landfill to the groundwater aquifer system, a potential hazard to both surrounding ecosystems and human populations.
Journal ArticleDOI
A combined approach to investigate the toxicity of an industrial landfill’s leachate: Chemical analyses, risk assessment and in vitro assays
Diego Baderna,Silvia Maggioni,Elena Boriani,Sabrina Gemma,Monica Molteni,Anna Lombardo,Andrea Colombo,S. Bordonali,Giuseppe Rotella,Marco Lodi,Emilio Benfenati +10 more
TL;DR: An integrated strategy to evaluate the toxicity of the leachate using chemical analyses, risk assessment guidelines and in vitro assays using the hepatoma HepG2 cells as a model was applied on an industrial waste landfill in northern Italy.
Journal ArticleDOI
Bioaccumulation of metals and effects of landfill pollution in small mammals. Part I. The greater white-toothed shrew, Crocidura russula
TL;DR: The considerable amounts of potentially toxic metals and the genotoxic effects indicate the harmful effect on biota and it is considered necessary biomonitoring this landfill sited in a partially protected area.
References
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Journal ArticleDOI
Chemical induction of sperm abnormalities in mice.
Andrew J. Wyrobek,W R Bruce +1 more
TL;DR: The results suggest that sperm abnormalities might provide a rapid inexpensive mammalian screen for agents that lead to errors in the differentiation of spermatogenic stem cells in vivo and thus indicate agents which might prove to be mutagenic, teratogenic, or carcinogenic.
Journal ArticleDOI
An evaluation of the mouse sperm morphology test and other sperm tests in nonhuman mammals: A report of the U.S. environmental protection agency Gene-Tox program
Andrew J. Wyrobek,Laurie Gordon,James G. Burkhart,Mary W. Francis,Robert W. Kapp,Gideon Letz,Heinrich V. Malling,John C. Topham,M.Donald Whorton +8 more
TL;DR: The mouse sperm morphology test has potential use for identifying chemicals that induce spermatogenic dysfunction and perhaps heritable mutations, and is found to be highly sensitive to germ-cell mutagens.
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Autoradiographic study of dna synthesis and the cell cycle in spermatogonia and spermatocytes of mouse testis using tritiated thymidine
TL;DR: The premeiotic synthesis of DNA takes place in primary spermatocytes during the resting phase and terminates just before the visible onset of the meiotic prophase.
Journal ArticleDOI
The genotoxicity of industrial wastes and effluents.
TL;DR: The evaluation of hazardous wastes and effluents by genotoxicity assays may provide data useful not only for hazard identification but for comparative risk assessment.
Journal ArticleDOI
The genetics of the mammalian gamete
TL;DR: Study of the association of the somatic and gametic genotypes with the gametic phenotype has been termed the genetics of gametes.