Antimicrobial Resistance and Virulence Genes of Escherichia coli Isolates from Swine in Ontario
Patrick Boerlin,Rebeccah M Travis,Carlton L. Gyles,Richard J. Reid-Smith,Richard J. Reid-Smith,Nicol Janecko,Heather Lim,Vivian M. Nicholson,Scott A. McEwen,Robert M. Friendship,Marie Archambault +10 more
Reads0
Chats0
TLDR
The results demonstrate that antimicrobial resistance epidemiology differs significantly between pathogenic and commensal E. coli isolates and may have important implications with regards to the spread and persistence of resistance and virulence genes in bacterial populations and to the prudent use of antimicrobial agents.Abstract:
A total of 318 Escherichia coli isolates obtained from diarrheic and healthy pigs in Ontario from 2001 to 2003 were examined for their susceptibility to 19 antimicrobial agents. They were tested by PCR for the presence of resistance genes for tetracycline, streptomycin, sulfonamides, and apramycin and of 12 common virulence genes of porcine E. coli. Antimicrobial resistance frequency among E. coli isolates from swine in Ontario was moderate in comparison with other countries and was higher in isolates from pigs with diarrhea than in isolates from healthy finisher pigs. Resistance profiles suggest that cephamycinases may be produced by ≥8% of enterotoxigenic E. coli (ETEC). Resistance to quinolones was detected only in enterotoxigenic E. coli (≤3%). The presence of sul3 was demonstrated for the first time in Canada in porcine E. coli isolates. Associations were observed among tetA, sul1, aadA, and aac(3)IV and among tetB, sul2, and strA/strB, with a strong negative association between tetA and tetB. The paa and sepA genes were detected in 92% of porcine ETEC, and strong statistical associations due to colocation on a large plasmid were observed between tetA, estA, paa, and sepA. Due at least in part to gene linkages, the distribution of resistance genes was very different between ETEC isolates and other porcine E. coli isolates. This demonstrates that antimicrobial resistance epidemiology differs significantly between pathogenic and commensal E. coli isolates. These results may have important implications with regards to the spread and persistence of resistance and virulence genes in bacterial populations and to the prudent use of antimicrobial agents.read more
Citations
More filters
Journal ArticleDOI
Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment.
TL;DR: This is the first study to comprehensively investigate the antibiotic resistance profile by analyzing the species of antibiotic-resistant bacteria and adopting qualitative and quantitative methods to investigate ARGs at a typical aquaculture area in northern China.
Journal ArticleDOI
Effects of the presence of sulfonamides in the environment and their influence on human health.
TL;DR: This review presents the most common types of sulfonamides used in healthcare and veterinary medicine and discusses the problems connected with their presence in the biosphere.
Journal ArticleDOI
Pathogenomics of the Virulence Plasmids of Escherichia coli
Timothy J. Johnson,Lisa K. Nolan +1 more
TL;DR: The evolution of these virulence plasmids and the implications of their acquisition by E. coli are now better understood and appreciated, with the available plasmid genomic sequences for several E. Escherichia coli pathotypes being compared in an effort to understand the evolution and define their core and accessory components.
Journal ArticleDOI
Antimicrobial resistance in Escherichia coli.
Laurent Poirel,Jean-Yves Madec,Agnese Lupo,Anne-Kathrin Schink,Nicolas Kieffer,Patrice Nordmann,Patrice Nordmann,Stefan Schwarz +7 more
TL;DR: Of note, coselection and persistence of resistances to critically important antimicrobial agents in human medicine also occurs through the massive use of antimicrobialagents in veterinary medicine, such as tetracyclines or sulfonamides, as long as all those determinants are located on the same genetic elements.
Journal ArticleDOI
Safety of raw meat and shellfish in Vietnam: an analysis of Escherichia coli isolations for antibiotic resistance and virulence genes.
TL;DR: The study has revealed that E. coli in raw foods is a significant reservoir of resistance and virulence genes.
References
More filters
Performance standards for antimicrobial susceptibility testing
TL;DR: The supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following Clinical and Laboratory Standards Institute (CLSI)–approved standards.
Journal ArticleDOI
National Committee for Clinical Laboratory Standards.
TL;DR: Many members of the Academy of Pediatrics seem to be generally unaware of the fact that the Academy has participated for ten years in a very interesting and valuable organization, the National Committee for Clinical Laboratory Standards (NCCLS).
Journal ArticleDOI
Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance
Ian Chopra,Marilyn C. Roberts +1 more
TL;DR: Changing the use of tetracyclines in human and animal health as well as in food production is needed if this class of broad-spectrum antimicrobials through the present century is to continue to be used.
Book
Clinical veterinary microbiology
TL;DR: General techniques laboratory safety collection and submission of diagnostic specimens essential equipment and reagents for a veterinary diagnostic microbiology laboratory diagnostic applications of immunological tests
Journal ArticleDOI
Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes.
TL;DR: A preliminary assessment of the amino acids which may be important in binding aminoglycosides was obtained from data and from the results of mutational analysis of several of the genes encoding am inoglycoside-modifying enzymes.