Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio.
Omar Ulises Cruz-Ramírez,Lucía Margarita Valenzuela-Salas,Alberto Blanco-Salazar,José Antonio Rodríguez-Arenas,Paris Astrid Mier-Maldonado,Juan Carlos García-Ramos,Nina Bogdanchikova,Alexey Pestryakov,Yanis Toledano-Magaña +8 more
TLDR
In this paper, the authors report the complete characterization of five polyvinylpyrrolidone-coated AgNP (PVP-AgNP) formulations, their cytotoxic activity against human colon tumor cells (HCT-15), their effect on primary mouse cultures, and their lethal dose on BALB/c mice.Abstract:
Silver nanoparticles (AgNPs) not only have shown remarkable results as antimicrobial and antiviral agents but also as antitumor agents. This work reports the complete characterization of five polyvinylpyrrolidone-coated AgNP (PVP-AgNP) formulations, their cytotoxic activity against human colon tumor cells (HCT-15), their cytotoxic effect on primary mouse cultures, and their lethal dose on BALB/c mice. The evaluated AgNP formulations have a composition within the ranges Ag: 1.14-1.32% w/w, PVP: 19.6-24.5% and H2O: 74.2-79.2% with predominant spherical shape within an average size range of 16-30 nm according to transmission electron microscopy (TEM). All formulations assessed increase mitochondrial ROS concentration and induce apoptosis as the leading death pathway on HCT-15 cells. Except for AgNP1, the growth inhibition potency of AgNP formulations of human colon tumor cancer cells (HCT-15) is 34.5 times higher than carboplatin, one of the first-line chemotherapy agents. Nevertheless, 5-10% of necrotic events, even at the lower concentration evaluated, were observed. The cytotoxic selectivity was confirmed by evaluating the cytotoxic effect on aorta, spleen, heart, liver, and kidney primary cultures from BALB/c mice. Despite the cytotoxic effects observed in vitro, the lethal dose and histopathological analysis showed the low toxicity of these formulations (all of them on Category 4 of the Globally Harmonized System of Classification and Labelling of Chemicals) and minor damage observed on analyzed organs. The results provide an additional example of the rational design of safety nanomaterials with antitumor potency and urge further experiments to complete the preclinical studies for these AgNP formulations.read more
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Photothermal Therapy with HER2-Targeted Silver Nanoparticles Leading to Cancer Remission
Victoria O. Shipunova,M. M. Belova,Polina A. Kotelnikova,Olga A. Shilova,Aziz B. Mirkasymov,N V Danilova,Elena N. Komedchikova,Rachela Popovtzer,Sergey M. Deyev,Maxim P. Nikitin +9 more
TL;DR: For the first time, it is shown that HER2-directed plasmonic Ag nanoparticles are effective sensitizers for targeted photothermal oncotherapy and resulted in full primary tumor regression and the prevention of metastatic spread.
Journal ArticleDOI
The Infectious Bronchitis Coronavirus Pneumonia Model Presenting a Novel Insight for the SARS-CoV-2 Dissemination Route.
Ekaterina Nefedova,Vyacheslav Koptev,Anna S. Bobikova,Viktoria Cherepushkina,Tatyana Mironova,V. N. Afonyushkin,Nikolai Shkil,Nikolai Donchenko,Yulia Kozlova,Natalia Sigareva,Natalia Davidova,Nina Bogdanchikova,Alexey Pestryakov,Yanis Toledano-Magaña +13 more
TL;DR: Infectious bronchitis (IB) of chickens is a highly contagious disease characterized by damage of the respiratory system and reproductive organs in young animals caused by a virus of the genus Gamma coronavirus.
Journal ArticleDOI
Effect of nano silver on gastroprotective activity against ethanol-induced stomach ulcer in rats.
Ibrahim Abdel Aziz Ibrahim,Abbas I. Hussein,Mahmoud S. Muter,Abdulalah T. Mohammed,Morteta H. Al-Medhtiy,Suhayla H. Shareef,Peshawa Yunis Aziz,Nabaz Fisal Shakir Agha,Mahmood Ameen Abdulla +8 more
TL;DR: In this article , the authors evaluated the gastroprotective effect of Ag NPs against ethanol-induced gastric ulcers in rats, and found that Ag nPs improved the adversative influence of ethanolinduced stomach damage as confirmed by declining ulcer index and raised the percentage of ulcer prevention.
Journal ArticleDOI
Treatment with Argovit® Silver Nanoparticles Induces Differentiated Postharvest Biosynthesis of Compounds with Pharmaceutical Interest in Carrot (Daucus carota L.).
Laura Sofia Santoscoy-Berber,Marilena Antunes-Ricardo,Melissa Zulahi Gallegos-Granados,Juan Carlos García-Ramos,Alexey Pestryakov,Yanis Toledano-Magaña,Nina Bogdanchikova,Rocio Alejandra Chavez-Santoscoy +7 more
TL;DR: In this article, five types of silver nanoparticles (AgNPs) were used to analyze the content of enhancement phenolic compounds in carrots, and the individual compounds were quantified by High Performance Liquid Chromatography (HPLC) and identified by HPLC-MS.
Journal ArticleDOI
Hypothetical Mechanism of Skin Argyria
Vasily Burmistrov,A. N. Burmistrov,G.V. Odegova,Alexey Pestryakov,Roberto Luna-Vázquez-Gómez,Nina Bogdanchikova +5 more
TL;DR: A hypothetical mechanism based on a perspiration system for delivering the silver compounds from the gastrointestinal tract to the skin for argyria and it was proposed not to completely exclude the partial participation of silver chloride along with the silver proteinates in this process.
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