Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) Function as Transcriptional Activators in Abscisic Acid Signaling
TLDR
Results indicate that both AtMYC2 and AtMYB2 proteins function as transcriptional activators in ABA-inducible gene expression under drought stress in plants.Abstract:
In Arabidopsis, the induction of a dehydration-responsive gene, rd22, is mediated by abscisic acid (ABA). We reported previously that MYC and MYB recognition sites in the rd22 promoter region function as cis-acting elements in the drought- and ABA-induced gene expression of rd22. bHLH- and MYB-related transcription factors, rd22BP1 (renamed AtMYC2) and AtMYB2, interact specifically with the MYC and MYB recognition sites, respectively, in vitro and activate the transcription of the β-glucuronidase reporter gene driven by the MYC and MYB recognition sites in Arabidopsis leaf protoplasts. Here, we show that transgenic plants overexpressing AtMYC2 and/or AtMYB2 cDNAs have higher sensitivity to ABA. The ABA-induced gene expression of rd22 and AtADH1 was enhanced in these transgenic plants. Microarray analysis of the transgenic plants overexpressing both AtMYC2 and AtMYB2 cDNAs revealed that several ABA-inducible genes also are upregulated in the transgenic plants. By contrast, a Ds insertion mutant of the AtMYC2 gene was less sensitive to ABA and showed significantly decreased ABA-induced gene expression of rd22 and AtADH1. These results indicate that both AtMYC2 and AtMYB2 proteins function as transcriptional activators in ABA-inducible gene expression under drought stress in plants.read more
Citations
More filters
Journal ArticleDOI
MYB transcription factors in Arabidopsis
TL;DR: The elucidation ofMYB protein function and regulation that is possible in Arabidopsis will provide the foundation for predicting the contributions of MYB proteins to the biology of plants in general.
Journal ArticleDOI
Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance
TL;DR: The present review summarizes the recent advances in elucidating stress-response mechanisms and their biotechnological applications and examines the following aspects: regulatory controls, metabolite engineering, ion transport, antioxidants and detoxification, late embryogenesis abundant (LEA) and heat-shock proteins.
Journal ArticleDOI
Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses.
TL;DR: This review article highlights transcriptional regulation of gene expression in response to drought and cold stresses, with particular emphasis on the role of transcription factors and cis-acting elements in stress-inducible promoters.
Journal ArticleDOI
Abscisic Acid: Emergence of a Core Signaling Network
TL;DR: A new model for ABA action has been proposed and validated, in which the soluble PYR/PYL/RCAR receptors function at the apex of a negative regulatory pathway to directly regulate PP2C phosphatases, which in turn directly regulate SnRK2 kinases.
Journal ArticleDOI
Drought and Salt Tolerance in Plants
TL;DR: The responses of plants to salt and water stress are described, the regulatory circuits which allow plants to cope with stress are presented, and how the present knowledge can be applied to obtain tolerant plants is discussed.
References
More filters
Journal ArticleDOI
Plant cellular and molecular responses to high salinity.
TL;DR: Evidence for plant stress signaling systems is summarized, some of which have components analogous to those that regulate osmotic stress responses of yeast, some that presumably function in intercellular coordination or regulation of effector genes in a cell-/tissue-specific context required for tolerance of plants.
Journal ArticleDOI
The molecular basis of dehydration tolerance in plants
J. Ingram,Dorothea Bartels +1 more
TL;DR: A large number of genes with a potential role in drought tolerance have been described, and major themes in the molecular response have been established.
Journal ArticleDOI
A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress.
TL;DR: The rd29A promoter in both transgenic Arabidopsis and tobacco was analyzed and a novel cis-acting, dehydration-responsive element (DRE) containing 9 bp, TACCGACAT, was identified that is involved in the first rapid response of rd 29A to conditions of dehydration or high salt.
Journal ArticleDOI
Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray.
Motoaki Seki,Mari Narusaka,Junko Ishida,Tokihiko Nanjo,Miki Fujita,Youko Oono,Asako Kamiya,Maiko Nakajima,Akiko Enju,Tetsuya Sakurai,Masakazu Satou,Kenji Akiyama,Teruaki Taji,Kazuko Yamaguchi-Shinozaki,Piero Carninci,Jun Kawai,Yoshihide Hayashizaki,Kazuo Shinozaki +17 more
TL;DR: A full-length cDNA microarray containing approximately 7000 independent, full- length cDNA groups is prepared to analyse the expression profiles of genes under drought, cold (low temperature) and high-salinity stress conditions over time, suggesting that various transcriptional regulatory mechanisms function in the drought,cold or high- salinity stress signal transduction pathways.
In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants
TL;DR: In this article, a nouvelle methode de transformation in situ par Agrobacterium is presented, based on l'infiltration sous vide de plantes d'Arabidopsis par une souche d'Agrobacteria contenant un vecteur binaire.