Patent
Articles having localized molecules disposed thereon and methods of producing same
David R. Rank,Jeffery Wegener,Jonas Korlach,Daniel B. Roitman,Yue Xu,John Lyle,Stephen Turner,Paul Peluso,Geoff Otto +8 more
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TLDR
In this paper, the authors employ elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate, which may include optical, chemical and/or mechanical processes for the deposition, removal, activation, and deactivation of chemical groups.Abstract:
Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.read more
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Patent
Compositions and methods for nucleic acid sequencing
TL;DR: In this paper, the use and preparation of template constructions for nucleic acid sequencing is described, as well as methods for their use in the development and preparation. But they do not discuss the use of these constructions in their application.
Patent
Two slow-step polymerase enzyme systems and methods
Keith Bjornson,Harold Lee,Jonas Korlach,Stephen Turner,Arek Bibillo,Fred Christians,Robin Emig,Molly He,Insil Park,Lei Jia,Andrei Fedorov,John Lyle +11 more
TL;DR: In this article, two slow step systems can be produced by selecting the appropriate polymerase enzyme, reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.
PatentDOI
Localization of near-field resonances in bowtie antennae: influence of adhesion layers
TL;DR: A plasmonic nanostructure for enhanced light excitation is described in this article, which consists of a substrate, an adhesion layer disposed on top of the substrate, a surface plasmmon resonance layer, and a cavity that extends into the surface resonance layer.
Patent
Generation of modified polymerases for improved accuracy in single molecule sequencing
Sonya Clark,Arek Bibillo,Paul Peluso,Fred Christians,Molly He,Insil Park,Harold Lee,Keith Bjornson,Lei Jia,Robin Emig +9 more
TL;DR: In this paper, the branching fractions of modified recombinant polymerases are compared to the branching fraction of the parent polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog.
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Waveguide-based detection system with scanning light source
TL;DR: In this article, a spatially scanning light source is used to generate optical pulses in one or more waveguides using a spatial scan light source, and a detection system, methods of use thereof and kits for detecting a biologically active analyte molecule are also provided.
References
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Journal ArticleDOI
Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites
TL;DR: In this article, a general approach for multilayers by consecutive adsorption of polyanions and polycations has been proposed and has been extended to other materials such as proteins or colloids.
Journal ArticleDOI
Self-assembled monolayers of thiolates on metals as a form of nanotechnology.
Journal ArticleDOI
Real-Time DNA Sequencing from Single Polymerase Molecules
John Eid,Adrian Fehr,Jeremy Gray,Khai Luong,John Lyle,Geoff Otto,Paul Peluso,David R. Rank,Primo Baybayan,Brad Bettman,Arkadiusz Bibillo,Keith Bjornson,Bidhan Chaudhuri,Fred Christians,Ronald L. Cicero,Sonya Clark,Ravindra V. Dalal,Alex DeWinter,John Dixon,Mathieu Foquet,Alfred Gaertner,Paul Hardenbol,Cheryl Heiner,Kevin Hester,David P. Holden,Gregory J. Kearns,Xiangxu Kong,Ronald Kuse,Yves Lacroix,Steven Lin,Paul Lundquist,Congcong Ma,Patrick Marks,Mark Maxham,Devon Murphy,Insil Park,Thang Pham,Michael Phillips,Joy Roy,Robert Sebra,Gene Shen,Jon M. Sorenson,Austin B. Tomaney,Kevin Travers,Mark Trulson,John Vieceli,Jeffrey Wegener,Dawn Wu,Alicia Yang,Denis Zaccarin,Peter Zhao,Frank Zhong,Jonas Korlach,Stephen Turner +53 more
TL;DR: Single-molecule, real-time sequencing data obtained from a DNA polymerase performing uninterrupted template-directed synthesis using four distinguishable fluorescently labeled deoxyribonucleoside triphosphates (dNTPs) are presented.
Patent
Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
TL;DR: In this paper, a polypeptide array can be synthesized on a substrate by attaching photoremovable groups to the surface of a substrate, exposing selected regions of the substrate to light to activate those regions, attaching an amino acid monomer with a photoregressive group to the activated regions, and repeating the steps of activation and attachment until the desired length and sequences are synthesized.
Journal ArticleDOI
Zero-Mode Waveguides for Single-Molecule Analysis at High Concentrations
Michael J. Levene,Jonas Korlach,Stephen Turner,Mathieu Foquet,Harold G. Craighead,Watt W. Webb +5 more
TL;DR: It is shown that arrays of zero-mode waveguides consisting of subwavelength holes in a metal film provide a simple and highly parallel means for studying single-molecule dynamics at micromolar concentrations with microsecond temporal resolution.
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Real-Time DNA Sequencing from Single Polymerase Molecules
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