Journal ArticleDOI
Assay of low deuterium enrichment of water by isotopic exchange with [U-13C3]acetone and gas chromatography-mass spectrometry
Dawei Yang,Frédérique Diraison,M. Beylot,Daniel Z. Brunengraber,Mark Atienza Samols,Vernon E. Anderson,Henri Brunengraber +6 more
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TLDR
The assay with [U-13C3]acetone allows measuring the 2H-enrichment of water even in biological samples containing acetone, which is more rapid and economical than the classical isotope ratio mass spectrometric assay of the enrichment of hydrogen gas derived from the reduction of water.About:
This article is published in Analytical Biochemistry.The article was published on 1998-05-01. It has received 113 citations till now. The article focuses on the topics: Acetone & Mass spectrometry.read more
Citations
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Journal ArticleDOI
FGF19 as a Postprandial, Insulin-Independent Activator of Hepatic Protein and Glycogen Synthesis
Serkan Kir,Sara A. Beddow,Varman T. Samuel,Paul E. Miller,Stephen F. Previs,Kelly Suino-Powell,H. Eric Xu,Gerald I. Shulman,Steven A. Kliewer,David J. Mangelsdorf +9 more
TL;DR: FGF19 treatment restored the loss of glycogen in diabetic animals lacking insulin and activates a physiologically important, insulin-independent endocrine pathway that regulates hepatic protein and glycogen metabolism.
Journal ArticleDOI
Contribution of hepatic de novo lipogenesis and reesterification of plasma non esterified fatty acids to plasma triglyceride synthesis during non-alcoholic fatty liver disease
TL;DR: In this paper, the authors measured triglyceride secretion rate and the contributions of hepatic lipogenesis and reesterification of non-esterified fatty acids (NEFA) to this secretion in healthy subjects and in patients with clear evidence of NAFLD.
Journal ArticleDOI
Inhibition of acetyl-CoA carboxylase suppresses fatty acid synthesis and tumor growth of non-small-cell lung cancer in preclinical models
Robert U. Svensson,Seth J. Parker,Lillian J. Eichner,Matthew J. Kolar,Martina Wallace,Sonja N. Brun,Portia S. Lombardo,Jeanine L. Van Nostrand,Amanda Hutchins,Lilliana Vera,Laurie Gerken,Jeremy R. Greenwood,Sathesh Bhat,Geraldine Harriman,William F. Westlin,H. James Harwood,Alan Saghatelian,Rosana Kapeller,Christian M. Metallo,Reuben J. Shaw +19 more
TL;DR: It is demonstrated that ACC mediates a metabolic liability of NSCLC and that ACC inhibition by ND-646 is detrimental toNSCLC growth, supporting further examination of the use of ACC inhibitors in oncology.
Journal ArticleDOI
Increased hepatic lipogenesis but decreased expression of lipogenic gene in adipose tissue in human obesity
TL;DR: Subjects with established obesity have an increased hepatic lipogenesis that could contribute to their excessive fat mass but no evidence for an increased lipogenic capacity of adipose tissue.
Journal ArticleDOI
Addition of inulin to a moderately high-carbohydrate diet reduces hepatic lipogenesis and plasma triacylglycerol concentrations in humans.
TL;DR: The addition of high-performance inulin to a moderately high-carbohydrate, low-fat diet has a beneficial effect on plasma lipids by decreasing hepatic lipogenesis and plasma triacylglycerol concentrations.
References
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Book
Introduction to organic chemistry
TL;DR: The covalent bond and shapes of molecules are discussed in detail in this paper, where Amino acids and proteins are represented as nucleophilic aliphatic substitution, and the reaction of benzene and phenols.
Book ChapterDOI
Solvent isotope effects of enzyme systems.
K B Schowen,R L Schowen +1 more
TL;DR: This chapter provides the physicochemical background for the use of solvent isotope effects in biochemical studies, with fairly complete derivations of the requisite algebraic expressions, an account of underlying assumptions, and a review of pertinent experimental and theoretical information.
Journal ArticleDOI
Measurement of de novo hepatic lipogenesis in humans using stable isotopes.
Marc K. Hellerstein,M. Christiansen,S. Kaempfer,C. Kletke,K. Wu,J. S. Reid,Kathleen Mulligan,N. S. Hellerstein,Cedric H.L. Shackleton +8 more
TL;DR: De novo hepatic lipogenesis is a quantitatively minor pathway, consistent with gas exchange estimates; fatty acid futile cycling (oxidation/resynthesis) is not thermogenically significant; and synthesis rates of different nonessential fatty acids by human liver are not identical in nonoverfed normal men.
Journal ArticleDOI
Deuterium and oxygen-18 measurements on microliter samples of urine, plasma, saliva, and human milk
TL;DR: Improved methods to measure 2H:1H and 18O:16O isotope ratios on microliter samples of biological fluids facilitate and extend the use of 2H and18O tracers in studies of body composition and energy expenditure.
Related Papers (5)
Measuring Lipogenesis and Cholesterol Synthesis in Humans with Deuterated Water: Use of Simple Gas Chromatographic/Mass Spectrometric Techniques
Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis
Robert R. Wolfe,David L. Chinkes +1 more