Journal ArticleDOI
Assessment of genotoxic and mutagenic potential of hexavalent chromium in the freshwater fish Labeo rohita (Hamilton, 1822).
Naresh Sahebrao Nagpure,Rashmi Srivastava,Ravindra Kumar,Basdeo Kushwaha,Satish K. Srivastava,Pavan Kumar,Anurag Dabas +6 more
TLDR
The study indicated hazardous effect of the hexavalent chromium to fish and other aquatic organisms and indirectly to human beings.Abstract:
The present study was undertaken to investigate the genotoxicity and mutagenicity of sublethal concentrations of hexavalent chromium (potassium dichromate) in the Indian major carp, Labeo rohita. The 96 h LC50 value of potassium dichromate estimated was 118 mg L−1 by probit analysis using SPSS (version 16.0) software. Based on 96 h LC50 value, three sublethal test concentrations of potassium dichromate (29.5, 59.0 and 88.5 mg L−1) were selected and specimens were exposed in vivo to these test concentrations for 96 h. The mutagenic and genotoxic effects of potassium dichromate were evaluated in gill and blood cells using micronucleus (MN) test and comet assay. In general, significant (p < 0.05) effects due to the concentrations and the exposure durations were observed in exposed specimens. The MN induction was highest at 96 h at all the test concentrations in the peripheral blood. A similar trend was observed for the DNA damage, measured in terms of percentage of tail DNA, in erythrocyte and gill c...read more
Citations
More filters
Journal ArticleDOI
Chromium Exposure Causes Structural Aberrations of Erythrocytes, Gills, Liver, Kidney, and Genetic Damage in Striped Catfish Pangasianodon hypophthalmus
Sajida Akter Suchana,Shaker Ahmed,SM Majharul Islam,Muhammad Lutfar Rahman,Muhammad Lutfar Rahman,Fazle Rohani,Tanzina Ferdusi,A K Shakur Ahmmad,Mst Kaniz Fatema,Muhammad Badruzzaman,Shahjahan +10 more
TL;DR: The study revealed that P. hypophthalmus is susceptible to Cr and can be used as a bio-indicator to assess aquatic metal pollution, and various hemato-biochemical parameters showed a significant decrease in hemoglobin, red blood cells, and blood glucose content, whereas white blood cells increased in Cr exposed fish.
Journal ArticleDOI
Mutagenic and histopathological effects of hexavalent chromium in tadpoles of Lithobates catesbeianus (Shaw, 1802) (Anura, Ranidae).
José Augusto do Nascimento Monteiro,Lorena Araújo da Cunha,Mary Helen Pestana da Costa,Herald Souza dos Reis,Aliceane Carolina da Silva Aguiar,Veronica Regina Lobato de Oliveira-Bahia,Rommel Rodríguez Burbano,Carlos Alberto Machado da Rocha +7 more
TL;DR: Hexavalent chromium was mutagenic to L. catesbeianus tadpoles and its toxic effects also resulted in anti-mitotic activity, besides inducing histopathological alterations in liver and kidney.
Journal Article
Assessment of pollution of river Ganges by tannery effluents using genotoxicity biomarkers in murrel fish, Channa punctatus (Bloch).
Naresh Sahebrao Nagpure,Rashmi Srivastava,Ravindra Kumar,Anurag Dabas,Basdeo Kushwaha,Pavan Kumar +5 more
TL;DR: The findings of the present analysis indicated contamination of river Ganges with tannery effluents which induce genotoxicity in fish with seasonal variation.
Journal ArticleDOI
Genotoxicity induced by hexavalent chromium leading to eryptosis in Ctenopharyngodon idellus
Kriti Handa,Rajinder Jindal +1 more
TL;DR: Genotoxicity induced by hexavalent chromium lead to eryptosis in C. idellus, and genes related to intrinsic apoptotic pathway induced by Cr(VI) presented significant upregulation in the expression of p53, Bax, Apaf-1, caspase9 and caspases3, and downregulation of Bcl2; inferring the initiation of apoptosis.
References
More filters
Journal ArticleDOI
A simple technique for quantitation of low levels of DNA damage in individual cells
TL;DR: Human lymphocytes were exposed to X-irradiation or treated with H2O2 and the extent of DNA migration was measured using a single-cell microgel electrophoresis technique under alkaline conditions and this technique appears to be sensitive and useful for detecting damage and repair in single cells.
Journal ArticleDOI
Fish bioaccumulation and biomarkers in environmental risk assessment: a review
TL;DR: There is little doubt that measurements of bioaccumulation and biomarker responses in fish from contaminated sites offer great promises for providing information that can contribute to environmental monitoring programs designed for various aspects of ERA.
Journal ArticleDOI
Oxidative mechanisms in the toxicity of metal ions
Sidney J. Stohs,Debasis Bagchi +1 more
TL;DR: Some mechanisms associated with the toxicities of metal ions are very similar to the effects produced by many organic xenobiotics, related to differences in solubilities, absorbability, transport, chemical reactions, and the complexes that are formed within the body.
Journal ArticleDOI
The single cell gel electrophoresis assay (comet assay): A European review
Valerie J. McKelvey-Martin,Michael H.L. Green,P. Schmezer,Beatrice L. Pool-Zobel,M.P. De Méo,Andrew Collins +5 more
TL;DR: The development of the SCGE assay, existing protocols for the detection and analysis of comets, the relevant underlying principles determining the behaviour of DNA, and the potential applications of the technique are reviewed.
Journal ArticleDOI
Fish micronuclei for assessing genotoxicity in water
Kabil Al-Sabti,Chris D. Metcalfe +1 more
TL;DR: The literature on the clastogenic effects of chemical and physical agents on fish cells, with emphasis on the induction of micronuclei in teleosts is reviewed, directing laboratories in the development of fish genotoxicity assays for water quality monitoring.