Journal ArticleDOI
C/EBP homologous protein is necessary for normal osteoblastic function.
Renata C. Pereira,Lisa Stadmeyer,Stefan J. Marciniak,David Ron,Ernesto Canalis,Ernesto Canalis +5 more
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TLDR
It is shown that chop null mice exhibit decreased bone formation and impaired osteoblastic function, indicating that CHOP is necessary for the normal expression of the osteoblast phenotype.Abstract:
C/EBP homologous protein (CHOP) suppresses adipogenesis and accelerates osteoblastogenesis in vitro. However, the effects of CHOP in the skeleton in vivo are not known. To investigate the actions of CHOP on bone remodeling, we examined the skeletal phenotype of chop null mice from 1 to 12 months of age. Chop null mice appeared normal and their growth and serum insulin like growth factor (IGF) I and osteocalcin levels were normal. X-ray analysis of the skeleton revealed no abnormalities and bone mineral density was normal. Static and dynamic histomorphometry revealed that chop null mice had decreased bone formation rates, without changes in osteoblast cell number, indicating an osteoblastic functional defect. The number of osteoblasts and osteoclasts and eroded surface were normal. Northern blot analysis revealed decreased type I collagen and osteocalcin mRNA levels in calvariae of chop null mice. In conclusion, chop null mice exhibit decreased bone formation and impaired osteoblastic function, indicating that CHOP is necessary for the normal expression of the osteoblastic phenotype. J. Cell. Biochem. © 2005 Wiley-Liss, Inc.read more
Citations
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Endoplasmic Reticulum Stress Signaling in Disease
Stefan J. Marciniak,David Ron +1 more
TL;DR: This review addresses aspects of the UPR currently understood to be involved in human disease, including their role in diabetes mellitus, atherosclerosis, and neoplasia.
Journal ArticleDOI
Networks and hubs for the transcriptional control of osteoblastogenesis
Jane B. Lian,Gary S. Stein,Amjad Javed,Andre J. van Wijnen,Janet L. Stein,Martin Montecino,Mohammad Q. Hassan,Tripti Gaur,Christopher J. Lengner,Daniel W. Young +9 more
TL;DR: An overview of the concepts of tissue-specific transcriptional control mechanisms essential for development of the bone cell phenotype is presented and the specificity of Runx2 regulatory activities provides a basis for novel therapeutic strategies to correct bone disorders.
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Bone morphogenetic proteins and their antagonists
TL;DR: Both intracellular and extracellular antagonists are regulated by BMPs, indicating the existence of local feedback mechanisms to modulate BMP cellular activities.
Journal ArticleDOI
Transcription factor C/EBPβ isoform ratio regulates osteoclastogenesis through MafB
TL;DR: Disequilibrium between bone‐forming osteoblasts and bone‐resorbing osteoclasts is central to many bone diseases and data show that dysregulated expression of translationally controlled isoforms of CCAAT/enhancer‐binding protein β (C/EBPβ) differentially affect bone mass.
Journal ArticleDOI
Distinct Cytoplasmic and Nuclear Functions of the Stress Induced Protein DDIT3/CHOP/GADD153
Alexandra Jauhiainen,Christer Thomsen,Linda Strömbom,Pernilla Grundevik,Carola Andersson,Anna Danielsson,Mattias K Andersson,Olle Nerman,Olle Nerman,Linda Rörkvist,Anders Ståhlberg,Pierre Åman +11 more
TL;DR: Characterization of DDIT3 regulated functions helps understanding its role in stress response and involvement in cancer and degenerative disorders.
References
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Journal ArticleDOI
Bone histomorphometry: Standardization of nomenclature, symbols, and units: Report of the asbmr histomorphometry nomenclature committee
A. Michael Parfitt,Marc K. Drezner,Francis H. Glorieux,John A. Kanis,Hartmut H. Malluche,Pierre J. Meunier,Susan M. Ott,Robert R. Recker +7 more
TL;DR: A committee of the Society to develop a unified system of termnology, suitable for adoption by the Journal of Bone and Mineral Research as part of its Instructions to Authors is formed, and is as complex and conceptually difficult as the field with which it deals.
Journal ArticleDOI
CHOP induces death by promoting protein synthesis and oxidation in the stressed endoplasmic reticulum
Stefan J. Marciniak,Chi Yun,Seiichi Oyadomari,Isabel Novoa,Yuhong Zhang,Rivka Jungreis,Kazuhiro Nagata,Heather P. Harding,David Ron +8 more
TL;DR: This work finds that CHOP directly activates GADD34, which promotes ER client protein biosynthesis by dephosphorylating phospho-Ser 51 of the alpha-subunit of translation initiation factor 2 (eIF2alpha) in stressed cells, and protects cells from ER stress by decreasing client protein load and changing redox conditions within the organelle.
Journal ArticleDOI
CCAAT/enhancer-binding proteins: structure, function and regulation
TL;DR: The structure, biological function and the regulation of the C/EBP family are reviewed, which have revealed an immense complexity with the potential existence of cell/tissue- and species-specific differences.
Journal ArticleDOI
Diabetes Mellitus and Exocrine Pancreatic Dysfunction in Perk−/− Mice Reveals a Role for Translational Control in Secretory Cell Survival
Heather P. Harding,Huiqing Zeng,Yuhong Zhang,Rivka Jungries,Peter Chung,Heidi Plesken,David D. Sabatini,David Ron +7 more
TL;DR: Findings suggest a special role for translational control in protecting secretory cells from ER stress in diabetes mellitus and exocrine pancreatic insufficiency.
Journal ArticleDOI
CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription factors C/EBP and LAP and functions as a dominant-negative inhibitor of gene transcription.
David Ron,Joel F. Habener +1 more
TL;DR: It is suggested that CHOP-10 is a negative modulator of the activity of C/EBP-like proteins in certain terminally differentiated cells, similar to the regulatory function of Id on theActivity of MyoD and MyOD-related proteins important in the development of muscle cells.
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CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription factors C/EBP and LAP and functions as a dominant-negative inhibitor of gene transcription.
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