Journal ArticleDOI
c-Myc-induced, long, noncoding H19 affects cell proliferation and predicts a poor prognosis in patients with gastric cancer.
TLDR
It is demonstrated that the altered expression of lncRNA H19, which is induced by c-Myc, is involved in the development and progression of GC by regulating cell proliferation and shows that H19 may be a potential diagnostic and prognostic target in patients with GC.Abstract:
Gastric cancer (GC) is one of the most frequent cancers worldwide. Recent studies have shown that long noncoding RNAs (lncRNAs) play critical roles in multiple biological processes, including oncogenesis. The present study aimed to evaluate the potential role of lncRNA H19 in GC. qRT-PCR was performed to investigate the expression of H19 in tumor tissues and corresponding non-tumor lung tissues from 80 patients with GC and in GC cell lines. The Kaplan-Meier method and Cox proportional hazards analysis were used to evaluate the association between H19 expression and overall survival time (OS). The biological significance of H19 was evaluated using siRNAs in vitro. We also constructed a c-Myc plasmid to investigate the cause of the altered expression of H19 in the progression of GC. The results show that lncRNA H19 is overexpressed in tumor tissues compared with adjacent normal tissues. An advanced tumor-node-metastasis stage was positively correlated with increased H19 expression (P < 0.001), and a high H19 expression was associated with poor OS and can be regarded as an independent predictor of the OS of GC patients (P = 0.042). MTT and colony formation assays confirmed that H19 expression affects GC cell proliferation in vitro. Furthermore, exogenous c-Myc significantly induces H19 expression, and the expression of H19 was positively correlated with the c-Myc levels in the 80 samples used in our study (Pearson correlation coefficient = -0.687). In conclusion, our study demonstrates that the altered expression of lncRNA H19, which is induced by c-Myc, is involved in the development and progression of GC by regulating cell proliferation and shows that H19 may be a potential diagnostic and prognostic target in patients with GC.read more
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Supplementary Data Only: Genomic binding by the Drosophila Myc, Max, Mad/Mnt transcription factor network.
Amir Orian,Bas van Steensel,Jeffrey J. Delrow,Harmen J. Bussemaker,Ling Li,Tomoyuki Sawado,Eleanor M. Williams,Lenora W. M. Loo,Shaun M. Cowley,Cynthia Yost,Sarah B. Pierce,Bruce A. Edgar,Susan M. Parkhurst,Robert N. Eisenman +13 more
TL;DR: In this paper, DamID was employed to carry out global genomic mapping of the Drosophila Myc, Max, and Mad/Mnt proteins, and the results suggest that a fundamental aspect of Max network function involves widespread binding and regulation of gene expression.
Journal ArticleDOI
Molecular mechanisms of long noncoding RNAs on gastric cancer
TL;DR: Interaction with DNA, RNA and proteins is involved in lncRNAs’ participation in gastric tumorigenesis and development.
Journal ArticleDOI
lncRNA GAS5 enhances G1 cell cycle arrest via binding to YBX1 to regulate p21 expression in stomach cancer
TL;DR: It is found that lncRNA GAS5 had lower expression in stomach cancer tissues than the normal counterparts, and the lncRNAs/YBX1/p21 pathway the authors discovered may provide useful targets for developing lnc RNA-based therapies for stomach cancer.
Journal ArticleDOI
Regulation of lncRNA and Its Role in Cancer Metastasis.
TL;DR: This review focuses on the mechanisms underlying the role of lncRNAs in cancer metastasis, which are mainly involved in the EMT process on the current literature.
Journal ArticleDOI
Long noncoding RNAs: novel insights into gastric cancer.
TL;DR: The regulation and functional role of lncRNAs in gastric cancer (GC) is outlined, the potential of lNCRNAs as prospective novel targets in GC treatment and biomarkers for GC diagnosis are discussed, and the lncRNA world is summarized.
References
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Journal ArticleDOI
Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals
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TL;DR: It is demonstrated that specific lincRNAs are transcriptionally regulated by key transcription factors in these processes such as p53, NFκB, Sox2, Oct4 (also known as Pou5f1) and Nanog, defining a unique collection of functional linc RNAs that are highly conserved and implicated in diverse biological processes.
Journal ArticleDOI
Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression
Ahmad M. Khalil,Mitchell Guttman,Maite Huarte,Manuel Garber,Arjun Raj,Dianali Rivea Morales,Kelly Thomas,Aviva Presser,Bradley E. Bernstein,Alexander van Oudenaarden,Aviv Regev,Eric S. Lander,John L. Rinn +12 more
TL;DR: A model in which some lincRNAs guide chromatin-modifying complexes to specific genomic loci to regulate gene expression is proposed, and it is shown that siRNA-mediated depletion of certain linc RNAs associated with PRC2 leads to changes in gene expression.