Cadmium influences the 5-Fluorouracil cytotoxic effects on breast cancer cells
Yolande Asara,Juan A. Marchal,Pasquale Bandiera,Vittorio Mazzarello,Lucia Gemma Delogu,Mario Sotgiu,Andrea Montella,Roberto Madeddu +7 more
TLDR
It is suggested that Cd prevents the cytotoxic effect of 5-FU on breast cancer cells, and could have an important clinical application in patients with breast cancer.Abstract:
The aim of the research was to evaluate a heavy metal, Cadmium (Cd), which was used to produce alterations in human breast cancer cell line MCF-7. Moreover, we analyzed both immunohistochemical and ultrastructural alterations induced by the antineoplastic drug, 5-Fluorouracil (5-FU), after exposure to different concentrations of Cadmium. Also, we compared the effects of these compounds on actin and tubulin cytoskeleton proteins. Under ultramicroscopic observation, control cells looked polymorphous with filopodia. In cells already treated with small concentrations of Cd, after brief times of incubation, we observed an intense metabolic activity with larger, clearer, and elongated mitochondria characterized by thin and numerous dilated cristae. 5-FU-treated cells showed cytotoxicity signs with presence of pore-like alterations in the cell membrane and evident degeneration of cytoplasm and cell nuclei. The addition of 5-FU (1.5 μM) to the cells treated with Cd (5 μM-20 μM) did not induce significant ultrastructural changes in comparison with cells treated only with Cd. In Cd+5FU-treated cells mitochondria with globular aspect and regular cristae indicated the active metabolic state. In cells treated only with Cd we observed alterations in actin distribution, while tubulin branched out throughout the cytoplasm. With the association of Cd+5FU, we observed less morphological alterations in both tubulin and actin cytoskeleton proteins. Although the mechanism remains unknown at present, our findings suggest that Cd prevents the cytotoxic effect of 5-FU on breast cancer cells. These preliminary results could have an important clinical application in patients with breast cancer.read more
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State of the evidence 2017: an update on the connection between breast cancer and the environment
TL;DR: Evidence from epidemiological studies, as well as a better understanding of mechanisms linking toxicants with development of breast cancer, all reinforce the conclusion that exposures to these substances – many of which are found in common, everyday products and byproducts – may lead to increased risk of developing breast cancer.
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Real-time imaging of microparticles and living cells with CMOS nanocapacitor arrays
Cecilia Laborde,Federico Pittino,Harrie A. Verhoeven,Serge G. Lemay,Luca Selmi,Maarten A. Jongsma,F. Widdershoven +6 more
TL;DR: It is shown that high-frequency impedance spectroscopy can be used to detect and image microparticles and living cells under physiological salt conditions and has the potential of the platform for label/tracer-free high-throughput screening of anti-tumour drug candidates.
Journal ArticleDOI
Cadmium modifies the cell cycle and apoptotic profiles of human breast cancer cells treated with 5-fluorouracil.
Yolande Asara,Juan A. Marchal,Esther Carrasco,Houria Boulaiz,Giuliana Solinas,Pasquale Bandiera,María Ángel García,Cristiano Farace,Andrea Montella,Roberto Madeddu +9 more
TL;DR: Results indicate that exposure to Cd blocks the anticancer effects of 5-FU in MCF-7 cells, which could have important clinical implications in patients treated with 5-Fluorouracil-based therapies and who are exposed to high levels of Cd.
Journal ArticleDOI
Cadmium, arsenic, selenium and iron- Implications for tumor progression in breast cancer.
Ewa Jabłońska,Katarzyna Socha,Edyta Reszka,Edyta Wieczorek,Jarosław Skokowski,Leszek Kalinowski,Wojciech Fendler,Barbara Seroczyńska,Marcin Wozniak,Maria H. Borawska,Wojciech Wasowicz +10 more
TL;DR: There was a significant positive correlation between Cd and As contents in tumor tissue, however, only Cd was significantly associated with histological type of tumor, its size, grading and progesterone receptor status.
Journal ArticleDOI
Effects of cadmium on Bcl-2/ Bax expression ratio in rat cortex brain and hippocampus.
TL;DR: Apoptotic effect of cadmium may be through the mitochondrial pathway by the activation of caspase-3/7 by induces cell apoptosis.
References
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Ann Cuypers,Michelle Plusquin,Tony Remans,Marijke Jozefczak,Els Keunen,Heidi Gielen,Kelly Opdenakker,Ambily Ravindran Nair,Elke Munters,Tom Artois,Tim S. Nawrot,Jaco Vangronsveld,Kelly Smeets +12 more
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Michael D. Johnson,Nicholas Kenney,Adriana Stoica,Leena Hilakivi-Clarke,Baljit Singh,Gloria Chepko,Robert Clarke,Peter F Sholler,Apolonio A Lirio,Colby Foss,Ronald Reiter,Bruce J. Trock,Soonmyoung Paik,Mary Beth Martin +13 more
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Cadmium is a mutagen that acts by inhibiting mismatch repair
Yong Hwan Jin,Alan B. Clark,Robbert J.C. Slebos,Hanan Al-Refai,Jack A. Taylor,Thomas A. Kunkel,Michael A. Resnick,Dmitry A. Gordenin +7 more
TL;DR: The mutation specificity along with responses in proofreading-deficient and MMR- deficient mutants indicate that cadmium reduces the capacity for MMR of small misalignments and base-base mismatches, and shows that a high level of genetic instability can result from environmental impediment of a mutation-avoidance system.
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Daily intake of arsenic, cadmium, mercury, and lead by consumption of edible marine species
TL;DR: The daily intake of arsenic (As), cadmium (Cd), mercury (Hg), and lead (Pb) through the consumption of 14 edible marine species by the general population of Catalonia, Spain, was estimated and health risks derived from this intake were assessed.
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