Journal ArticleDOI
CDK activity sensors: genetically encoded ratiometric biosensors for live analysis of the cell cycle.
Reads0
Chats0
TLDR
CDK activity sensors are utilized for their ability to visualize the exact moment of cell-cycle commitment, which has provided a breakthrough in understanding the proliferation-quiescence decision.Abstract:
Cyclin-dependent kinase (CDK) sensors have facilitated investigations of the cell cycle in living cells. These genetically encoded fluorescent biosensors change their subcellular location upon activation of CDKs. Activation is primarily regulated by their association with cyclins, which in turn trigger cell-cycle progression. In the absence of CDK activity, cells exit the cell cycle and become quiescent, a key step in stem cell maintenance and cancer cell dormancy. The evolutionary conservation of CDKs has allowed for the rapid development of CDK activity sensors for cell lines and several research organisms, including nematodes, fish, and flies. CDK activity sensors are utilized for their ability to visualize the exact moment of cell-cycle commitment. This has provided a breakthrough in understanding the proliferation-quiescence decision. Further adoption of these biosensors will usher in new discoveries focused on the cell-cycle regulation of development, ageing, and cancer.read more
Citations
More filters
Posted ContentDOI
A reevaluation of the relationship between EGL-43 (EVI1/MECOM) and LIN-12 (Notch) during C. elegans anchor cell invasion
Michael A. Q. Martinez,Angelina A. Mullarkey,Callista Yee,Chris Z. Zhao,Wan Zhang,Kang Shen,David Q. Matus +6 more
TL;DR: A heterologous co-expression system called AIDHB is designed and implemented that combines the auxin-inducible degron (AID) system of plants with a live cell-cycle sensor based on human DNA helicase B (DHB) and revealed that LIN-12 is not required for AC proliferation following loss of EGL-43, which contrasts with a double RNAi experiment directed against these same targets.
Journal ArticleDOI
Reevaluating the relationship between EGL-43 (EVI1) and LIN-12 (Notch) during C. elegans anchor cell invasion
Michael A. Q. Martinez,Angelina A. Mullarkey,Callista Yee,Chris Z. Zhao,Wan Zhang,Kang Shen,David Q. Matus +6 more
TL;DR: In this paper , a heterologous co-expression system called AIDHB that combines the auxin-inducible degron (AID) system of plants with a live cell-cycle sensor based on human DNA helicase B (DHB) was designed and implemented to reexamine the relationship between EGL-43 and LIN-12.
Posted ContentDOI
Live-cell imaging provides direct evidence for a threshold in CDK activity at the G2/M transition
TL;DR: In this paper , the dynamics of CDK activity in fission yeast and mammalian cells were investigated using a newly developed biosensor, Eevee-spCDK, based on Förster Resonance Energy Transfer (FRET).
References
More filters
Journal ArticleDOI
NIH Image to ImageJ: 25 years of image analysis
TL;DR: The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.
Journal ArticleDOI
Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression
Asako Sakaue-Sawano,Hiroshi Kurokawa,Toshifumi Morimura,Aki Hanyu,Hiroshi Hama,Hatsuki Osawa,Saori Kashiwagi,Kiyoko Fukami,Takaki Miyata,Hiroyuki Miyoshi,Takeshi Imamura,Masaharu Ogawa,Hisao Masai,Atsushi Miyawaki +13 more
TL;DR: Time-lapse imaging is performed to explore the spatiotemporal patterns of cell-cycle dynamics during the epithelial-mesenchymal transition of cultured cells, the migration and differentiation of neural progenitors in brain slices, and the development of tumors across blood vessels in live mice.
Journal ArticleDOI
A Restriction Point for Control of Normal Animal Cell Proliferation
TL;DR: Evidence is given here that cells are put into the same quiescent state by each of these diverse blocks to proliferation and that cells escape at the same point in G(1) of the cell cycle when nutrition is restored.
Journal ArticleDOI
Progressive Activation of CyclinB1-Cdk1 Coordinates Entry to Mitosis
Olivier Gavet,Jonathon Pines +1 more
TL;DR: It is demonstrated that different levels of CyclinB1-Cdk1 kinase activity trigger different mitotic events, thus revealing how the remarkable reorganization of the cell is coordinated at mitotic entry.
Journal ArticleDOI
Topologically associating domains and chromatin loops depend on cohesin and are regulated by CTCF, WAPL, and PDS5 proteins
Gordana Wutz,Csilla Várnai,Kota Nagasaka,David A. Cisneros,Roman R. Stocsits,Wen Tang,Stefan Schoenfelder,Gregor Jessberger,Matthias Muhar,M. Julius Hossain,Nike Walther,Birgit Koch,Moritz Kueblbeck,Jan Ellenberg,Johannes Zuber,Peter Fraser,Peter Fraser,Jan-Michael Peters +17 more
TL;DR: It is shown that cohesin suppresses compartments but is required for TADs and loops, that CTCF defines their boundaries, and that the cohes in unloading factor WAPL and its PDS5 binding partners control the length of loops.