Cell lines established by a temperature-sensitive simian virus 40 large-T-antigen gene are growth restricted at the nonpermissive temperature.
P S Jat,Phillip A. Sharp +1 more
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The thermolabile large T antigen, encoded by the simian virus 40 early-region mutant tsA58, was used to establish clonal cell lines derived from rat embryo fibroblasts that showed rapidly arrested growth and the inability of these cell lines to divide at the nonpermissive temperature was not readily complemented by the exogenous introduction of other nuclear oncogenes.Abstract:Â
The thermolabile large T antigen, encoded by the simian virus 40 early-region mutant tsA58, was used to establish clonal cell lines derived from rat embryo fibroblasts. These cell lines grew continuously at the permissive temperature but upon shift-up to the nonpermissive temperature showed rapidly arrested growth. The growth arrest occurred in either the G1 or G2 phase of the cell cycle. After growth arrest, the cells remained metabolically active as assayed by general protein synthesis and the ability to exclude trypan blue. The inability of these cell lines to divide at the nonpermissive temperature was not readily complemented by the exogenous introduction of other nuclear oncogenes. This finding suggests that either these genes establish cells via different pathways or that immortalization by one oncogene results in a finely balanced cellular state which cannot be adequately complemented by another establishment gene.read more
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The product of the retinoblastoma susceptibility gene has properties of a cell cycle regulatory element.
James A. DeCaprio,John W. Ludlow,Dennis C. Lynch,Yusuke Furukawa,James D. Griffin,Helen Piwnica-Worms,Chun-Ming Huang,David M. Livingston +7 more
TL;DR: The cell cycle-dependent phosphorylation of Rb is demonstrated and a model to explain how Rb may suppress cell growth by acting as a cell cycle regulatory element is proposed.
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Isolation, Characterization, and use of Stem Cells from the CNS
TL;DR: Because in vitro culture conditions are unlikely to provide all of the factors necessary for inducing the proliferation and differentiation of neural precursors, recent studies have explored the properties of well-characterized precursor populations after implantation back into specific regions of the developing or adult CNS.
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Cooperation between oncogenes.
TL;DR: The question addressed here is why multiple events are needed for carcinogenesis and how the expression of oncogenes and the loss of growth suppressor cooperate in this process.
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Direct derivation of conditionally immortal cell lines from an H-2Kb-tsA58 transgenic mouse.
Parmjit S. Jat,Mark Noble,Paris Ataliotis,Yujiro Tanaka,Nikos Yannoutsos,Lena Larsen,Dimitris Kioussis +6 more
TL;DR: Since previous studies have shown that TAg expression in transgenic mice is associated with tumorigenesis and aberrant development, a thermolabile TAg was utilized to reduce the levels of functional TAg present in vivo to circumvent the need for gene insertion in vitro to generate cell lines.
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Stabilization of the p53 tumor suppressor is induced by adenovirus 5 E1A and accompanies apoptosis.
Scott W. Lowe,H E Ruley +1 more
TL;DR: The results suggest that the involvement of p53 in tumor suppression and/or apoptosis can be regulated at the level of protein turnover, and a major oncogenic role for E1B is to counter cellular responses to E1A that preclude transformation by E 1A alone.
References
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