Cellular and Molecular Barriers to Gene Transfer by a Cationic Lipid
Reads0
Chats0
TLDR
The results indicate that endocytosis was the major mechanism of entry in cationic lipid-mediated gene transfer and suggest that attention to specific steps in the cellular process may further improve the efficiency of transfection and increase its use in a number of applications.About:
This article is published in Journal of Biological Chemistry.The article was published on 1995-08-11 and is currently open access. It has received 1446 citations till now. The article focuses on the topics: Cationic liposome & Transfection.read more
Citations
More filters
Journal ArticleDOI
Size-dependent internalization of particles via the pathways of clathrin-and caveolae-mediated endocytosis
TL;DR: The data indicate that the size itself of (ligand-devoid) particles can determine the pathway of entry in non-phagocytic B16 cells, and kinetic parameters may determine the almost exclusive internalization of such particles along this pathway rather than via caveolae.
Journal ArticleDOI
Pathophysiology and management of pulmonary infections in cystic fibrosis.
TL;DR: This comprehensive State of the Art review summarizes the current published knowledge base regarding the pathophysiology and microbiology of pulmonary disease in cystic fibrosis and potential future therapies.
Journal ArticleDOI
Uptake Pathways and Subsequent Intracellular Trafficking in Nonviral Gene Delivery
TL;DR: The different uptake pathways that are involved in nonviral gene delivery from a gene delivery point of view are reviewed and available knowledge concerning cellular entry and the intracellular trafficking of cationic lipid-DNA complexes (lipoplexes) and cationsic polymer- DNA complexes (polyplexes) is summarized.
Journal ArticleDOI
An inverted hexagonal phase of cationic liposome-DNA complexes related to DNA release and delivery
TL;DR: Optical microscopy revealed that the LalphaC complexes bind stably to anionic vesicles (models of cellular membranes), whereas the more transfectant HIIC complexes are unstable and rapidly fuse and release DNA upon adhering to anionics.
Journal ArticleDOI
Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection.
Yuhong Xu,Francis C. Szoka +1 more
TL;DR: It is proposed that after the cationic lipid/DNA complex is internalized into cells by endocytosis it destabilization induces flip-flop of anionic lipids from the cytoplasmic-facing monolayer, which laterally diffuse into the complex and form a charge neutral ion pair with the cationsic lipids.
References
More filters
Journal ArticleDOI
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure
Philip L. Felgner,Thomas R. Gadek,Marilyn Holm,Richard Bolton Roman,Hardy W. Chan,Michael Wenz,Jeffrey P. Northrop,Gordon M. Ringold,Mark Danielsen +8 more
TL;DR: Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.
Journal ArticleDOI
Histochemical methods for acid phosphatase using hexazonium pararosanilin as coupler
Tibor Barka,Paul J. Anderson +1 more
TL;DR: ‘[he use of freslsbv diazotizecl pararosanilin w-itim a-msaphthyb pisosphate its a sinmultaneous coupling azo dye method for acid phosphatase resulted in significant improveumment because of the nmaumy desirable characteristics of the final azo dyed (1, 2).
Journal ArticleDOI
Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations
Jiin Felgner,Raj Kumar,C.N. Sridhar,Carl J. Wheeler,Y. J. Tsai,Border Richard C,P. Ramsey,M. Martin,Philip L. Felgner +8 more
TL;DR: The studies reported here examine the effects of some systematic chemical structural changes in both the cationic lipid components and the neutral phospholipid requirements for transfection activity in COS.
Journal ArticleDOI
Cationic liposome-mediated transfection
TL;DR: A lipid named DOTMA has been created that forms unilamellar liposomes which complex with DNA and RNA for the transfection of mammalian cells, including suspension cells and hybridomas.
Journal ArticleDOI
High efficiency transformation by direct microinjection of DNA into cultured mammalian cells.
TL;DR: Direct microinjection of DNA by glass micropipettes was used to introduce the Herpes simplex virus thymidine kinase gene into cultured mammalian cells, and transformation frequency was relatively insensitive to DNA concentration and did not depend on co-injecting with a carrier DNA.
Related Papers (5)
Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection.
Yuhong Xu,Francis C. Szoka +1 more