Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations
Jiin Felgner,Raj Kumar,C.N. Sridhar,Carl J. Wheeler,Y. J. Tsai,Border Richard C,P. Ramsey,M. Martin,Philip L. Felgner +8 more
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TLDR
The studies reported here examine the effects of some systematic chemical structural changes in both the cationic lipid components and the neutral phospholipid requirements for transfection activity in COS.About:Â
This article is published in Journal of Biological Chemistry.The article was published on 1994-01-28 and is currently open access. It has received 1467 citations till now. The article focuses on the topics: Cationic liposome & Liposome.read more
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Liposomal drug delivery systems: from concept to clinical applications.
TL;DR: Lipidic nanoparticles are the first nanomedicine delivery system to make the transition from concept to clinical application, and they are now an established technology platform with considerable clinical acceptance.
Journal ArticleDOI
Nonviral Vectors for Gene Delivery
TL;DR: Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells.
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Toxicity of cationic lipids and cationic polymers in gene delivery.
TL;DR: The structural features of cationic compounds are evaluated and the relationship of toxicity and structure is summarized and available suggestions are provided to provide available suggestions on the development of these cATIONic compounds.
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Micellar nanocarriers: pharmaceutical perspectives.
TL;DR: This review will discuss some recent trends in using micelles as pharmaceutical carriers, including lipid-core micells, which may become the imaging agents of choice in different imaging modalities.
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Cellular and Molecular Barriers to Gene Transfer by a Cationic Lipid
TL;DR: The results indicate that endocytosis was the major mechanism of entry in cationic lipid-mediated gene transfer and suggest that attention to specific steps in the cellular process may further improve the efficiency of transfection and increase its use in a number of applications.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure
Philip L. Felgner,Thomas R. Gadek,Marilyn Holm,Richard Bolton Roman,Hardy W. Chan,Michael Wenz,Jeffrey P. Northrop,Gordon M. Ringold,Mark Danielsen +8 more
TL;DR: Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.
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Cationic liposome-mediated transfection
TL;DR: A lipid named DOTMA has been created that forms unilamellar liposomes which complex with DNA and RNA for the transfection of mammalian cells, including suspension cells and hybridomas.
Journal ArticleDOI
A novel cationic liposome reagent for efficient transfection of mammalian cells
Xiang Gao,Leaf Huang +1 more
TL;DR: A novel cationic derivative of cholesterol, 3 beta [N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol), has been synthesized and used to prepare sonicated liposomes with dioleoylphosphatidylethanolamine and facilitates efficient DNA mediated transfection in A431 human epidermoid carcinoma cells.
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Theory of self-assembly of lipid bilayers and vesicles
TL;DR: A unified theory is developed that explains the formation of bilayers and vesicles and accounts quantitatively for many of their physical properties: properties including vesicle size distributions and bilayer elasticity emerge from a unified theory that links thermodynamics, interaction free energy, and molecular geometry.
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A novel cationic liposome reagent for efficient transfection of mammalian cells
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