Journal ArticleDOI
Characterization of adenohypophysial polypeptides by two-dimensional gel electrophoresis: II. Sulfated and glycosylated polypeptides
Patrizia Rosa,A. Zanini +1 more
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TLDR
The available evidence suggests that this might not be the only subcellular location of the sulfated polypeptide in the pituitary tissue, and that this sulfated component was present in purified bovine prolactin granules stripped of their limiting membrane.About:
This article is published in Molecular and Cellular Endocrinology.The article was published on 1981-11-01. It has received 88 citations till now. The article focuses on the topics: Anterior pituitary & Polyacrylamide gel electrophoresis.read more
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The Chromogranin–Secretogranin Family
TL;DR: The members of the chromogranin–secretogran in family of peptide hormones, biogenic amines, and neurotransmitters are enclosed within vesicles in the neuroendocrine system and a variety of neurons.
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The molecular function of adrenal chromaffin granules: Established facts and unresolved topics
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Synaptophysin and chromogranins/secretogranins-widespread constituents of distinct types of neuroendocrine vesicles and new tools in tumor diagnosis
TL;DR: This review article discusses the biochemical, cell biological and molecular biological data obtained so far for two major types of NE markers, synaptophysin, which is characteristic of the small “transparent-looking” neurosecretory vesicles, and the chromogranin/secretogranins, which are widespread constituents of the larger “dense-cored” secretory granules.
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The primary structure of human secretogranin II, a widespread tyrosine-sulfated secretory granule protein that exhibits low pH- and calcium-induced aggregation.
Hans-Hermann Gerdes,Patrizia Rosa,E Phillips,Patrick A. Baeuerle,Rainer Frank,P Argos,Wieland B. Huttner +6 more
TL;DR: The results suggest that the low pH- and calcium-induced aggregation of secretogranin II may be important for the organization of the secretory granule matrix and raise the possibility that aggregation ofsecretog Granin I may be involved in its sorting to secretorygranules.
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Secretoneurin-a neuropeptide generated in brain, adrenal medulla and other endocrine tissues by proteolytic processing of secretogranin II (chromogranin C)
TL;DR: The highest concentrations of secretogranin 154-186 are found in the hypothalamus, two- to six-fold lower levels are present in the hippocampus, caudate nucleus, thalamus and brainstem and these concentrations are comparable to those of established neuropeptides.
References
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A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
TL;DR: A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.
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Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
TL;DR: It is demonstrated that pre-exposure of the film to a brief flash of light greatly increases the sensitivity of fluorography, because it corrects the non-linear relationship between radioactivity of the sample and absorbance of theFilm image.
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High resolution two-dimensional electrophoresis of basic as well as acidic proteins
TL;DR: This paper describes an alternate procedure for the first dimension which, unlike isoelectric focusing, resolves basic as well as acidic proteins, and involves a short time of electrophoresis toward the cathode and separates most proteins according to their isoelection points.
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Preferential Release of Newly Synthesized Prolactin Granules Is the Result of Functional Heterogeneity among Mammotrophs
TL;DR: It is concluded that preferential release of newly synthesized PRL is due to preferential discharge of Newly synthesized granules, since release occurs at a time when labeled hormone is already in the granules and there is no evidence that the established secretory pathway is bypassed.