Characterization of PLGA Microparticles as a Drug Carrier for 3-Ethoxycarbonyl-2H-Benzofuro[3,2-f]-1-Benzopyran-2-One. Ultrastructural Study of Cellular Uptake and Intracellular Distribution
Anderson J. Gomes,Adriana S Faustino,Antonio E.H. Machado,Maria Elisabete Darbello Zaniquelli,Thais de Paula Rigoletto,Claure N. Lunardi,Laurelucia O. Lunardi +6 more
TLDR
Results indicate that PLGA MP could be a promising delivery system for psoralen in connection with ultraviolet irradiation therapy (PUVA) and the intracellular distribution and cellular uptake of the drug by using an encapsulation technique for therapeutic optimization.Abstract:
Here we describe the application of microparticles (MPs) for the delivery and release of the drug a benzopsoralen. We also evaluated the intracellular distribution and cellular uptake of the drug by using an encapsulation technique for therapeutic optimization. MPs containing the compound 3-ethoxycarbonyl-2H-benzofuro[3,2-f]-1-benzopyran-2-one (psoralen A) were prepared by the solvent evaporation technique, and parameters such as particle size, drug encapsulation efficiency, effect of the encapsulation process on the drug's photochemistry, zeta potential, external morphology, and in vitro release behavior were evaluated. The intracellular distribution of MPs as well as their uptake by tissues were monitored. Size distribution studies using dynamic ligh scattering and scanning electron microscopy revealed that the MPs are spherical in shape with a diameter of 1.4 micro m. They present low tendency toward aggregation, as confirmed by their zeta potential (+10.6 mV). The loading efficiency obtained was 75%. As a consequence of the extremely low diffusivity of the drug in aqueous medium, the drug release profile of the MPs in saline phosphate buffer (pH 7.4) was much slower than that obtained in the biological environment. Among the population of peritoneal phagocytic cells, only macrophages were able to phagocytose poly-d,l-lactic-co-glycolic acid (PLGA) MP. The use of psoralen A in association with ultraviolet light (360 nm) revealed morphological characteristics of cell damage such as cytoplasmic vesiculation, mitochondria condensation, and swelling of both the granular endoplasmatic reticulum and the nuclear membrane. These results indicate that PLGA MP could be a promising delivery system for psoralen in connection with ultraviolet irradiation therapy (PUVA).read more
Citations
More filters
Journal ArticleDOI
Strategies for enhanced photodynamic therapy effects.
TL;DR: Two broad approaches for PDT enhancement are focused on: mechanism‐based combination treatments in which PDT and a second modality can be designed to either increase the susceptibility of tumor cells to PDT or nullify the treatment outcome‐mitigating molecular responses triggered by PDT of tumors.
Journal ArticleDOI
Poly(lactide-co-glycolide)-rifampicin nanoparticles efficiently clear Mycobacterium bovis BCG infection in macrophages and remain membrane-bound in phago-lysosomes.
Raja Kalluru,Federico Fenaroli,David Westmoreland,Lilia S. Ulanova,Atoosa Maleki,Norbert Roos,Marie Paulsen Madsen,Gerbrand Koster,Wolfgang Egge-Jacobsen,Steven Ray Wilson,Hanna Roberg-Larsen,Gopal K. Khuller,Amandeep Singh,Bo Nyström,Gareth Griffiths +14 more
TL;DR: It is argued that PLGA NPs remain membrane-enclosed in macrophages for at least 13 days and degrade slowly, and one dose given after infection is sufficient to efficiently clear the BCG infection after 9–12 days of treatment, as shown by estimates of the number of bacterial colonies in vitro.
Journal ArticleDOI
Immobilized ruthenium complexes and aspects of their reactivity
Elia Tfouni,Fabio Gorzoni Doro,Anderson J. Gomes,Roberto Santana da Silva,Gustavo Metzker,Patricia Graça Zanichelli Benini,Douglas Wagner Franco +6 more
TL;DR: In this paper, the authors describe methods for the characterization of ruthenium complexes into/onto functionalized silica gel, zeolites, polymers, dendrimers, sol-gel, nano and microparticles.
Journal ArticleDOI
Nanoparticle-mediated delivery of the antimicrobial peptide plectasin against Staphylococcus aureus in infected epithelial cells
TL;DR: E encapsulation of plectasin into PLGA-based nanoparticles appears to be a viable strategy to improve the efficacy of pLECTasin against infections in epithelial tissues.
Journal ArticleDOI
Microencapsulation of inorganic nanocrystals into PLGA microsphere vaccines enables their intracellular localization in dendritic cells by electron and fluorescence microscopy.
Christopher Schliehe,Constanze Schliehe,Marc Thiry,Ulrich I. Tromsdorf,Joachim Hentschel,Horst Weller,Marcus Groettrup +6 more
TL;DR: A novel strategy for the efficient encapsulation of inorganic nanocrystals (NCs) into PLGA-MS as a tool to study their intracellular localization and argues against an escape of PLga-MS from the endosome as has previously been suggested as a mechanism to facilitate cross-presentation of PL GA-MS encapsulated antigen.
References
More filters
Journal ArticleDOI
Preparation and characterization of cationic PLGA nanospheres as DNA carriers.
TL;DR: From the gel electrophoresis studies, it is found that the charge on the nanospheres is sufficient to efficiently bind the negatively charged DNA electrostatically and could serve as potential alternatives of the existing negatively charged nanoparticles.
Journal ArticleDOI
Biodegradable microspheres in drug delivery.
Hiroaki Okada,Hajime Toguchi +1 more
TL;DR: General aspects of biodegradable microspheres prepared from natural and synthesized polymers used in drug delivery systems and studies on antitumor therapy by chemoembolization using PLGA microsphere containing an angiogenesis inhibitor are described.
Journal ArticleDOI
Microparticle formation and its mechanism in single and double emulsion solvent evaporation
TL;DR: This study investigates the mechanism of the solvent elimination from the emulsion droplets and its influence on the particle morphology, encapsulation and release behavior of microparticles in double emulsion formulations.
Journal ArticleDOI
Influence of the surface properties on nanoparticle-mediated transport of drugs to the brain.
TL;DR: Poly(alkyl cyanoacrylate) nanoparticles enable the delivery of a number of drugs, including doxorubicin, loperamide, tubocurarine, the NMDA receptor antagonist MRZ 2/576, and the peptides dalargin and kytorphin across the blood-brain barrier (BBB) after coating with surfactants.
Journal ArticleDOI
Preparation and characterization of poly(lactic acid)-poly(ethylene glycol)-poly(lactic acid) (PLA-PEG-PLA) microspheres for controlled release of paclitaxel.
Gang Ruan,Si-Shen Feng +1 more
TL;DR: PLA-PEG-PLA microspheres could be promising for the clinical administration of highly hydrophobic antineoplastic drugs such as paclitaxel with hypothesis that incorporation of a hydrophilic PEG segment within the Hydrophobic PLA might facilitate the pac litaxel release.
Related Papers (5)
Biodegradable nanoparticles for drug and gene delivery to cells and tissue
Jayanth Panyam,Vinod Labhasetwar +1 more