Journal ArticleDOI
Cholesterol is required for Leishmania donovani infection: implications in leishmaniasis
Reads0
Chats0
TLDR
It is reported here that cholesterol depletion from macrophage plasma membranes using methyl-beta-cyclodextrin (MbetaCD) results in a significant reduction in the extent of leishmanial infection, and the reduction of the ability of the parasite to infect host macrophages can be reversed upon replenishment of cell membrane cholesterol.About:
This article is published in Molecular and Biochemical Parasitology.The article was published on 2004-02-01. It has received 117 citations till now. The article focuses on the topics: Lipid raft & Leishmania donovani.read more
Citations
More filters
Journal ArticleDOI
The Serotonin1A Receptor: A Representative Member of the Serotonin Receptor Family
TL;DR: The ligand binding activity and G-protein coupling of the receptor is modulated by membrane cholesterol thereby indicating the requirement of cholesterol in maintaining the receptor organization and function, raises important questions on the role of membrane lipids and domains in the function of this receptor.
Journal ArticleDOI
Leishmania and the macrophage: a multifaceted interaction.
TL;DR: The complexity of the host-parasite interactions is detailed and the latest understanding of key events that make Leishmania such a successful intracellular parasite is summarized.
Journal ArticleDOI
Membrane Cholesterol: a Crucial Molecule Affecting Interactions of Microbial Pathogens with Mammalian Cells
P. Goluszko,Bogdan Nowicki +1 more
TL;DR: Several microorganisms and bacterial products target lipid rafts, membrane microdomains of eukaryotic cells enriched in cholesterol, which is a major sterol of mammalian cells.
Journal ArticleDOI
Leishmania donovani Affects Antigen Presentation of Macrophage by Disrupting Lipid Rafts
TL;DR: In this paper, a low peptide dose of exogenous peptide was applied to splenic macrophages and the membrane fluidity of P388D1(I) was reduced by exposing the cell below phase transition point or loading cholesterol into membrane.
Journal ArticleDOI
Role of cholesterol in parasitic infections
TL;DR: Present review of literature shows that parasites induce significant changes in lipid parameters, as has been shown in the in vitro study where substitution of serum by lipid/cholesterol in medium and in experimental models (in vivo).
References
More filters
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Journal ArticleDOI
Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
Journal ArticleDOI
Lipid rafts and signal transduction
Kai Simons,Derek Toomre +1 more
TL;DR: It is now becoming clear that lipid micro-environments on the cell surface — known as lipid rafts — also take part in this process of signalling transduction, where protein–protein interactions result in the activation of signalling cascades.
Journal ArticleDOI
CNS synaptogenesis promoted by glia-derived cholesterol
Daniela H. Mauch,Karl Nägler,Stefan Schumacher,Christian Göritz,Eva-Christina Müller,Albrecht Otto,Frank W. Pfrieger +6 more
TL;DR: Cholesterol complexed to apolipoprotein E-containing lipoproteins may explain the delayed onset of CNS synaptogenesis after glia differentiation and neurobehavioral manifestations of defects in cholesterol or lipoprotein homeostasis.
Journal ArticleDOI
GPI-anchored proteins are organized in submicron domains at the cell surface
Rajat Varma,Satyajit Mayor +1 more
TL;DR: The results indicate that lipid-linked proteins are organized in cholesterol-dependent submicron-sized domains, which are likely to be less than 70 nm in diameter and are disrupted by removal of cellular cholesterol.
Related Papers (5)
Essential Role for Cholesterol in Entry of Mycobacteria into Macrophages
John Gatfield,Jean Pieters +1 more
Raft membrane domains: from a liquid-ordered membrane phase to a site of pathogen attack.
F. G. van der Goot,Thomas Harder +1 more