Cloning of the gene coding for human L apoferritin.
Claudio Santoro,Maria Marone,Marina Ferrone,Francesco Costanzo,Maurizio Colombo,Carlo Minganti,Riccardo Cortese,Lorenzo Silengo +7 more
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TLDR
Data is reported suggesting that a single gene is mainly expressed in several tissues examined, and its sequence shows three introns that are identical to that of cDNA clone isolated from human liver.Abstract:
A recently reported cDNA clone coding for human promyelocytic L apoferritin, shows some differences with a liver L apoferritin cDNA. We have investigated if these differences are due to the expression of different genes or to an alternative transcription of an unique gene. In this paper we report data suggesting that a single gene is mainly expressed in several tissues examined. This gene has been cloned and characterized. Its sequence shows three introns: the exon sequence is identical to that of cDNA clone isolated from human liver. A minimum of five related pseudogenes have been also analysed. One of them is a processed pseudogene interrupted by an intron-like fragment.read more
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An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs
TL;DR: 5'-Noncoding sequences have been compiled from 699 vertebrate mRNAs and GCCA/GCCATGG emerges as the consensus sequence for initiation of translation in vertebrates.
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The ferritins: molecular properties, iron storage function and cellular regulation☆
Pauline M. Harrison,Paolo Arosio +1 more
TL;DR: A great deal of research effort is now concentrated on two aspects of ferritin: its functional mechanisms and its regulation and the apparent links between iron and citrate metabolism through a single molecule with dual function are described.
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The molecular mechanisms of the metabolism and transport of iron in normal and neoplastic cells.
Des R. Richardson,Prem Ponka +1 more
TL;DR: Iron uptake by mammalian cells is mediated by the binding of serum Tf to the TfR, and recent work has suggested that the short-lived messenger molecule, NO, can affect cellular Fe metabolism via its interaction with IRP1.
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Iron-responsive elements: regulatory RNA sequences that control mRNA levels and translation
John L. Casey,Matthias W. Hentze,David M. Koeller,SW Caughman,Tracey A. Rouault,Richard D. Klausner,Joe B. Harford +6 more
TL;DR: The 3' untranslated region of the mRNA for the human TfR was shown to be necessary and sufficient for iron-dependent control of mRNA levels and an mRNA element has been implicated in the mediation of distinct regulatory phenomena dependent on the context of the element within the transcript.
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A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs
TL;DR: expression and functional analysis establish that individual gene products can bind to GCCAAT recognition sites and serve both as promoter-selective transcriptional activators and as initiation factors for DNA replication.
References
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DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids
Arnold J. Berk,Phillip A. Sharp +1 more
TL;DR: A simple and sensitive method for detecting, sizing and mapping RNA transcripts from viral or cloned DNAs has been developed and used to examine the cytoplasmic transcripts produced during the early phase of adenovirus 2 (Ad2) infection of HeLa cells.
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Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
TL;DR: In adding the restriction endonuclease cleavage sites for SphI and KpnI to the lac cloning region of the phage vectors, this technique can be used as a general method for inserting sequences of DNA as well as introducing deletions and base pair changes.
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Lambda replacement vectors carrying polylinker sequences
TL;DR: A new family of lambda replacement vectors and derivatives containing amber mutations and derivatives of the EMBL3 vector allow the application of genetic screening procedures based on selection for the products of homologous recombination events, and for the selective cloning of DNA sequences linked to supF genes.