Journal ArticleDOI
Collagen heterogeneity in human cartilage: Identification of several new collagen chains
TLDR
These findings imply further molecular heterogeneity of vertebrate collagens, and the existence of at least 9 different structural genes for collagen chains.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1979-04-27. It has received 296 citations till now. The article focuses on the topics: Collagen, type I, alpha 1 & Type II collagen.read more
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Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels
Paul D. Benya,Joy D. Shaffer +1 more
TL;DR: Using SDS-polyacrylamide gel electrophoresis of intact collagen chains and two-dimensional cyanogen bromide peptide mapping, this work demonstrated a complete return to the differentiated collagen phenotype and demonstrates a reversible system for the study of gene expression.
Journal ArticleDOI
Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors.
Henry E. Young,Timothy A. Steele,Timothy A. Steele,Robert A. Bray,John A. Hudson,Julie A. Floyd,Kristina C. Hawkins,Karen Thomas,Troy Austin,Chris Edwards,Jeremy Cuzzourt,Mary Duenzl,Paul A. Lucas,Asa C. Black +13 more
TL;DR: This study details the profile of 13 cell surface cluster differentiation markers on human reserve stem cells derived from connective tissues as well as lineage‐uncommitted pluripotent stem cells capable of forming muscle, adipocytes, cartilage, bone, fibroblasts, and endothelial cells.
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Cartilage contains mixed fibrils of collagen types II, IX, and XI.
TL;DR: The distribution of collagen XI in fibril fragments from 17-d chick embryo sternal cartilage was determined by immunoelectron microscopy using specific polyclonal antibodies and implies that collagen XI is an integral component of the cartilage fibrillar network and homogeneously distributed throughout the tissue.
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Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues.
TL;DR: Results are consistent with earlier biochemical analyses, which showed type X collagen to be a product of that subpopulation of chondrocytes that have undergone hypertrophy, and either it or an immunologically cross- reactive molecule is also present in bone, and exhibits a diminished fluorescent intensity as compared with hypertrophic cartilage.
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Characterization of the antibody response in mice with type II collagen-induced arthritis, using monoclonal anti-type II collagen antibodies.
TL;DR: Twenty monoclonal antibodies reactive with type II collagen were characterized as to their determinant specificity and their reactivity with cartilage-derived components and selected autoreactive antibodies caused synovitis, but in no case did it give rise to full-blown arthritis.
References
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Journal ArticleDOI
Biochemical characteristics and biological significance of the genetically-distinct collagens.
TL;DR: Current information on the biochemical properties, biosynthesis, and tissue distribution of Type I, II, and III collagens is summarized with special reference to possible unique functional roles fulfilled by each of thesecollagens.
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Isolation of a collagen from basement membranes containing three identical α-chains
TL;DR: Chromatographic studies on CM-cellulose of collagens isolated from basement membranes of the glomerulus, lens capsule and Descemet's membrane indicate that the molecule is composed of three identical α -1 chains.
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Collagen synthesis in normal and osteoarthritic human cartilage.
TL;DR: The data suggest that collagen synthesis in these tissues is substantially greater than in nonosteoarthritic tissues and varies directly with the severity of the disease process up to a point and then varies inversely as the lesion becomes more severe.
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Isolation of two distinct collagens from chick cartilage.
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Differences in Collagen Metabolism between Normal and Osteoarthritic Human Articular Cartilage
Marcel Nimni,Kalindi Deshmukh +1 more
TL;DR: Normal human articular cartilage synthesizes only one type of a chain, which exhibits the chromatographic behavior of the αl(II) chains described for chick and bovine cartilage, while osteoarthritic cartilage generates in addition a collagen containing α2 chains and β components.