Journal ArticleDOI
Comparative efficacy of experimental anthrax vaccine candidates against inhalation anthrax in rhesus macaques.
Bruce E. Ivins,Margaret L. Pitt,P.F. Fellows,Joseph Farchaus,G. E. Benner,David M. Waag,Stephen F. Little,G.W. Anderson,Paul H. Gibbs,Arthur M. Friedlander +9 more
TLDR
The authors examined the efficacy of Bacillus anthracis protective antigen (PA) combined with adjuvants as vaccines against an aerosol challenge of virulent anthrax spores in rhesus macaques.About:
This article is published in Vaccine.The article was published on 1998-07-01. It has received 243 citations till now. The article focuses on the topics: Anthrax vaccines & Anthrax Vaccine Adsorbed.read more
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Journal ArticleDOI
Tracking human antigen-specific memory B cells: a sensitive and generalized ELISPOT system.
TL;DR: This assay is used to demonstrate that the anthrax vaccine (AVA; BioThrax) elicits a substantial population of protective-antigen (PA) specific memory B cells, and these B cells satisfy the canonical surface phenotype of humanMemory B cells.
Journal ArticleDOI
Binary Bacterial Toxins: Biochemistry, Biology, and Applications of Common Clostridium and Bacillus Proteins
TL;DR: This review comprehensively surveys the literature and discusses the similarities and distinct differences between each Clostridium and Bacillus binary toxin in terms of their biochemistry, biology, genetics, structure, and applications in science and medicine.
Journal ArticleDOI
Search for correlates of protective immunity conferred by anthrax vaccine.
Shaul Reuveny,Moshe D. White,Yaakov Adar,Yaron Kafri,Zeev Altboum,Yehusha Gozes,David Kobiler,Avigdor Shafferman,Baruch Velan +8 more
TL;DR: Results clearly demonstrate that neutralizing antibodies to PA constitute a major component of the protective immunity against anthrax and suggest that this parameter could be used as a surrogate marker for protection.
Journal ArticleDOI
Efficacy of a human anthrax vaccine in guinea pigs, rabbits, and rhesus macaques against challenge by Bacillus anthracis isolates of diverse geographical origin
P.F. Fellows,M.K. Linscott,Bruce E. Ivins,Margaret L. Pitt,Cynthia A. Rossi,Paul H. Gibbs,Arthur M. Friedlander +6 more
TL;DR: It is demonstrated that, although AVA confers variable protection against different B. anthracis isolates in guinea pigs, it is highly protective against these same isolate in both rabbits and rhesus macaques.
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Squalene emulsions for parenteral vaccine and drug delivery.
TL;DR: The chemical structure and sources of squalene, a linear triterpene that is extensively utilized as a principal component of parenteral emulsions for drug and vaccine delivery, are presented and the physicochemical and biological properties ofSqualene-containing emulsion products are evaluated in the context of paretteral formulations.
References
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Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
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Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill
TL;DR: It was shown that phenol red does not interfere with the measurements and no washing steps are required since all ingredients can be added subsequently, and Serum proteins at concentrations up to 25% have no influence on the result.
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Plaque formation and isolation of pure lines with poliomyelitis viruses.
Renato Dulbecco,Marguerite Vogt +1 more
TL;DR: Pure virus lines were established by isolating the virus population produced in single plaques, which had the same morphological, serological, and pathogenic properties as the parent strain.
Journal Article
Separation and characterization of saponins with adjuvant activity from Quillaja saponaria Molina cortex.
TL;DR: Several distinct saponins were demonstrated to boost antibody levels by 100-fold or more when used in mouse immunizations with the Ag BSA and beef liver cytochrome b5, and vary considerably in their toxicity, as assessed by lethality in mice.
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Rapid colormetric assay for cell viability: Application to the quantitation of cytotoxic and growth inhibitory lymphokines
TL;DR: The data indicate that the L929 cell line was 10-50-fold more sensitive than the ESH -5L line to the lytic activity of cytotoxins produced by human phytohemagglutinin-P-activated T lymphocytes, or the cytotoxin produced by peripheral blood lymphocytes stimulated with various tumor cell lines.