Journal ArticleDOI
Comparative structural analysis of eukaryotic flagella and cilia from Chlamydomonas, Tetrahymena, and sea urchins
Gaia Pigino,Aditi Maheshwari,Aditi Maheshwari,Khanh Huy Bui,Khanh Huy Bui,Chikako Shingyoji,Shinji Kamimura,Takashi Ishikawa +7 more
TLDR
Comparing the axonemal structure of three different organisms with widely divergent bending-motions by electron cryo-tomography defines the diversity of molecular architectures in these organisms, and forms the basis for future correlation with their different bending-Motions.About:
This article is published in Journal of Structural Biology.The article was published on 2012-05-01. It has received 86 citations till now. The article focuses on the topics: Axoneme & Undulipodium.read more
Citations
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Structural biology in situ--the potential of subtomogram averaging.
TL;DR: The method, called subtomogram averaging or subvolume averaging, allows structures of macromolecular complexes to be resolved in situ, and it is speculated how the method will solve more structures at higher resolution, allowing in situ structural biology.
Journal ArticleDOI
Polarity and asymmetry in the arrangement of dynein and related structures in the Chlamydomonas axoneme
TL;DR: Cryoelectron tomography and subtomogram averaging reveal a high degree of structural asymmetry and polarization in dynein localization in the Chlamydomonas flagella.
Journal ArticleDOI
Motility and more: the flagellum of Trypanosoma brucei.
Gerasimos Langousis,Kent L. Hill +1 more
TL;DR: The structure, assembly and function of the trypanosome flagellum is discussed, including canonical roles incell motility as well as novel and emerging roles in cell morphogenesis and host–parasite interactions.
Journal ArticleDOI
Axoneme Structure from Motile Cilia
TL;DR: This article reviews the recent progress of structural studies of the axoneme and its components using electron microscopy and X-ray crystallography, mainly focusing on motile cilia and gives insights into the force generation mechanism of dynein, ciliary bending mechanism, ciliogenesis, and evolution of theAxoneme.
Journal ArticleDOI
Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry
Paul Guichard,Virginie Hachet,Norbert Majubu,Aitana Neves,Davide Demurtas,Natacha Olieric,Isabelle Flückiger,Akinori Yamada,Kumiko Kihara,Yuichiro Nishida,Shigeharu Moriya,Michel O. Steinmetz,Yuichi Hongoh,Pierre Gönczy +13 more
TL;DR: This work provides unprecedented insight into the architecture of the centriole proximal region, which is key for a thorough understanding of the mechanisms governing centrioles assembly.
References
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UCSF Chimera--a visualization system for exploratory research and analysis.
Eric F. Pettersen,Thomas D. Goddard,Conrad C. Huang,Gregory S. Couch,Daniel M. Greenblatt,Elaine C. Meng,Thomas E. Ferrin +6 more
TL;DR: Two unusual extensions are presented: Multiscale, which adds the ability to visualize large‐scale molecular assemblies such as viral coats, and Collaboratory, which allows researchers to share a Chimera session interactively despite being at separate locales.
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Computer Visualization of Three-Dimensional Image Data Using IMOD
TL;DR: IMOD is useful for studying and modeling data from tomographic, serial section, and optical section reconstructions and allows image data to be visualized by several different methods.
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Spider and web : processing and visualization of images in 3d electron microscopy and related fields
Joachim Frank,Michael Radermacher,Michael Radermacher,Pawel A. Penczek,Jun Zhu,Jun Zhu,Yanhong Li,Mahieddine Ladjadj,Ardean Leith +8 more
TL;DR: Novel features are a suite of operations relating to the determination, modeling, and correction of the contrast transfer function and the availability of the entire documentation in hypertext format.
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Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi.
Donald S. Gorman,R. P. Levine +1 more
TL;DR: Evidence is presented for its participation and site of action with respect to cytochrome f in the photosynthetic electron transport chain of C. reinhardi, a mutant strain that lacks the copper protein, plastocyanin.
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Dual-axis tomography: an approach with alignment methods that preserve resolution.
TL;DR: Analysis by Fourier sector correlation indicated that the variable tilt increment improved the reconstruction in some respects but degraded it in others, and a varying tilt increment thus does not give an unqualified improvement, at least when using back-projection algorithms for the reconstruction.