Journal ArticleDOI
Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging
Pietro Ferraro,Sergio De Nicola,Andrea Finizio,Giuseppe Coppola,Simonetta Grilli,Carlo Magro,Giovanni Pierattini +6 more
TLDR
An approach is proposed for removing the wavefront curvature introduced by the microscope imaging objective in digital holography, which otherwise hinders the phase contrast imaging at reconstruction planes and it is shown that a correction effect can be obtained at all reconstruction planes.Abstract:
An approach is proposed for removing the wave front curvature introduced by the microscope imaging objective in digital holography, which otherwise hinders the phase contrast imaging at reconstruction planes. The unwanted curvature is compensated by evaluating a correcting wave front at the hologram plane with no need for knowledge of the optical parameters, focal length of the imaging lens, or distances in the setup. Most importantly it is shown that a correction effect can be obtained at all reconstruction planes. Three different methods have been applied to evaluate the correction wave front and the methods are discussed in detail. The proposed approach is demonstrated by applying digital holography as a method of coherent microscopy for imaging amplitude and phase contrast of microstructures.read more
Citations
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Journal ArticleDOI
Phase aberration compensation via a self-supervised sparse constraint network in digital holographic microscopy
TL;DR: Zhang et al. as mentioned in this paper proposed a self-supervised sparse constraint network (SSCNet) for phase aberration compensation without the perturbation hypothesis, as well as an additional label or mask.
Proceedings ArticleDOI
Dual-wavelength digital holographic microscopy with sub-nanometer axial accuracy
Jonas Kühn,Florian Charrière,Tristan Colomb,Frédéric Montfort,Etienne Cuche,Yves Emery,Pierre Marquet,Christian Depeursinge +7 more
TL;DR: In this article, a modified dual-wavelength Digital Holographic Microscopy (DHM) measurements on a certified 8.9 nm high Chromium thin step sample and demonstrate sub-nanometer axial accuracy.
Proceedings ArticleDOI
Phase reconstruction of biological cells by digital microscopic image plane holography
TL;DR: In this article, the phase distribution of the object is covered with the aberration induced by the microscope objective, and to recover the phase of the cell, Zernike polynomials are used to eliminate the aberrations.
Proceedings ArticleDOI
Imaging and characterization of surface relief gratings on azopolymer by digital holographic microscopy
Alejandro Calabuig,Carmela Rianna,Carmela Rianna,Vito Pagliarulo,Maurizio Ventre,Maurizio Ventre,Silvia Cavalli,Simonetta Grilli,Paolo A. Netti,Paolo A. Netti,Pietro Ferraro +10 more
TL;DR: In this article, surface relief gratings (SRGs) were realized with Lloyd's mirror system and optical characterization of the material was performed, starting from a commercial one and using a new way to analyse SRGs by means of Digital Holography Microscopy, to determine relevant parameters for the realization of the patterns with different shape and size.
Proceedings ArticleDOI
Automatic Compensation of Phase Aberrations in Digital Holographic Microscopy for Ovarian Cancer Cell Drug Sensitivity Survey by Detecting Object-free Regions on Synthetic Difference Image
Leiping Che,Wen Xiao,Feng Pan +2 more
TL;DR: Method enables automatic compensation of phase aberrations of digital holographic microscopy by detecting object-free regions on synthetic difference image is proposed and applied to monitor the response of ovarian cancer cells exposed to cisplatin.
References
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Journal ArticleDOI
Phase-shifting digital holography
Ichirou Yamaguchi,Tong Zhang +1 more
TL;DR: A new method is proposed in which the distribution of complex amplitude at a plane is measured by phase-shifting interferometry and then Fresnel transformed by a digital computer, which can reconstruct an arbitrary cross section of a three-dimensional object with higher image quality and a wider viewing angle than from conventional digital holography using an off-axis configuration.
Journal ArticleDOI
Direct recording of holograms by a CCD target and numerical reconstruction.
Ulf Schnars,W. Jüptner +1 more
TL;DR: The principle of recording holograms directly on a CCD target is described and a real image of the object is reconstructed from the digitally sampled hologram by means of numerical methods.
Journal ArticleDOI
Digital holography for quantitative phase-contrast imaging.
TL;DR: A new application of digital holography for phase-contrast imaging and optical metrology and an application to surface profilometry shows excellent agreement with contact-stylus probe measurements.
Journal ArticleDOI
Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
TL;DR: Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation to show that the transverse resolution is equal to the diffraction limit of the imaging system.
Journal ArticleDOI
Digital recording and numerical reconstruction of holograms
Ulf Schnars,Werner Jüptner +1 more
TL;DR: The principles and major applications of digital recording and numerical reconstruction of holograms (digital holography) are described, which are applied to measure shape and surface deformation of opaque bodies and refractive index fields within transparent media.
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