Journal ArticleDOI
Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging
Pietro Ferraro,Sergio De Nicola,Andrea Finizio,Giuseppe Coppola,Simonetta Grilli,Carlo Magro,Giovanni Pierattini +6 more
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TLDR
An approach is proposed for removing the wavefront curvature introduced by the microscope imaging objective in digital holography, which otherwise hinders the phase contrast imaging at reconstruction planes and it is shown that a correction effect can be obtained at all reconstruction planes.Abstract:
An approach is proposed for removing the wave front curvature introduced by the microscope imaging objective in digital holography, which otherwise hinders the phase contrast imaging at reconstruction planes. The unwanted curvature is compensated by evaluating a correcting wave front at the hologram plane with no need for knowledge of the optical parameters, focal length of the imaging lens, or distances in the setup. Most importantly it is shown that a correction effect can be obtained at all reconstruction planes. Three different methods have been applied to evaluate the correction wave front and the methods are discussed in detail. The proposed approach is demonstrated by applying digital holography as a method of coherent microscopy for imaging amplitude and phase contrast of microstructures.read more
Citations
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Journal ArticleDOI
Principles and techniques of digital holographic microscopy
TL;DR: Digital holography is an emerging field of new paradigm in general imaging applications as discussed by the authors, and a review of a subset of the research and development activities in digital holographic microscopy techniques and applications is presented.
Journal ArticleDOI
Digital holographic microscopy for live cell applications and technical inspection
Björn Kemper,Gert von Bally +1 more
TL;DR: Digital holographic microscopy enables a quantitative phase contrast metrology that is suitable for the investigation of reflective surfaces as well as for the marker-free analysis of living cells.
Journal ArticleDOI
High-resolution quantitative phase-contrast microscopy by digital holography
TL;DR: Techniques of digital holography are improved in order to obtain high-resolution, high-fidelity images of quantitative phase-contrast microscopy, and the angular spectrum method of calculating holographic optical field is seen to have significant advantages including tight control of spurious noise components.
Journal ArticleDOI
Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation.
Tristan Colomb,Etienne Cuche,Florian Charrière,Jonas Kühn,Nicolas Aspert,Frédéric Montfort,Pierre Marquet,Christian Depeursinge +7 more
TL;DR: A procedure that compensates for phase aberrations in digital holographic microscopy by computing a polynomial phase mask directly from the hologram, which enables one to reconstruct correct and accurate phase distributions, even in the presence of strong and high-order aberration.
Journal ArticleDOI
Numerical parametric lens for shifting, magnification, and complete aberration compensation in digital holographic microscopy
Tristan Colomb,Frédéric Montfort,Jonas Kühn,Nicolas Aspert,Etienne Cuche,Anca Marian,Florian Charrière,Sébastien Bourquin,Pierre Marquet,Christian Depeursinge +9 more
TL;DR: It is shown that operations usually performed by optical components and described in ray geometrical optics, such as image shifting, magnification, and especially complete aberration compensation, can be mimicked by numerical computation of a NPL.
References
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Journal ArticleDOI
Three-dimensional microscopy with phase-shifting digital holography
Tong Zhang,Ichirou Yamaguchi +1 more
TL;DR: In this paper, the authors applied phase-shifting digital holography to microscopy by deriving the complex amplitude of light scattered from microscopic 3D objects through a microscope objective by video camera recording, phase shifting analysis, and computer reconstruction.
Journal ArticleDOI
Direct phase determination in hologram interferometry with use of digitally recorded holograms
TL;DR: In this paper, the phase determination in hologram interferometry is performed using a CCD target and phase estimation from the digitally sampled holograms, which are then used to calculate the phase of the interference phase.
Journal ArticleDOI
Whole optical wavefields reconstruction by digital holography.
Simonetta Grilli,Pietro Ferraro,S. De Nicola,Andrea Finizio,Giovanni Pierattini,Riccardo Meucci +5 more
TL;DR: It is shown that this technique can be efficiently used for obtaining quantitative information from the intensity and the phase distributions of the reconstructed field at different locations along the propagation direction.
Journal Article
Digital decoding of in-line holograms
Levent Onural,Peter D. Scott +1 more
TL;DR: The proposed filter is a truncated series expansion of the inverse of that operator that maps object opacity function to hologram intensity, which is shown to be equivalent to conventional (optical) reconstruction, with successive terms increasingly sup-pressing the twin image.
Journal ArticleDOI
Digital Decoding Of In-Line Holograms
Levent Onural,Peter D. Scott +1 more
TL;DR: In this paper, a truncated series expansion of the inverse operator that maps object opacity function to hologram intensity was proposed, which is shown to be equivalent to conventional (optical) reconstruction, with successive terms increasingly supressing the twin image.
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