Construction of GFP vectors for use in Gram‐negative bacteria other than Escherichia coli
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A set of vectors containing a mutated gfp gene was constructed for use with Gram-negative bacteria other than Escherichia coli, and two plasmids containing gfp expressed from a lac and an npt2 promoter were constructed.Abstract:
A set of vectors containing a mutated gfp gene was constructed for use with Gram-negative bacteria other than Escherichia coli. These constructs were: pTn3gfp for making random promoter probe gfp insertions into cloned DNA in E. coli for subsequent introduction into host strains; pUTmini-Tn5gfp for making random promoter probe gfp insertions directly into host strains; p519gfp and p519nfp, broad host range mob+ plasmids containing gfp expressed from a lac and an npt2 promoter, respectively.read more
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Book ChapterDOI
Genetic approaches to study of biofilms
George A. O'Toole,Leslie A. Pratt,Paula I. Watnick,Dianne K. Newman,Valerie B. Weaver,Roberto Kolter +5 more
TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
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Shewanella oneidensis MR-1 nanowires are outer membrane and periplasmic extensions of the extracellular electron transport components
Sahand Pirbadian,Sarah E. Barchinger,Kar Man Leung,Hye Suk Byun,Yamini Jangir,Rachida Bouhenni,Samantha B. Reed,Margaret F. Romine,Daad A. Saffarini,Liang Shi,Yuri A. Gorby,John H. Golbeck,Mohamed Y. El-Naggar +12 more
TL;DR: Using in vivo fluorescence measurements, immunolabeling, and quantitative gene expression analysis, it is demonstrated that S. oneidensis MR-1 nanowires are extensions of the outer membrane and periplasm that include the multiheme cytochromes responsible for EET, rather than pilin-based structures as previously thought.
Journal ArticleDOI
A panel of Tn7-based vectors for insertion of the gfp marker gene or for delivery of cloned DNA into Gram-negative bacteria at a neutral chromosomal site
TL;DR: To demonstrate the utility of a dual marker/reporter system, the Tn7-gfp marker system was combined with a Tn5-delivered luxAB reporter system in Pseudomonas fluorescens and allowed detection of gfp-tagged cells in the barley rhizosphere, while expression of the TN5- tagged locus could be determined by measuring bioluminescence.
Journal ArticleDOI
Genetic and molecular evidence that the Pseudomonas syringae type III effector protein AvrRpt2 is a cysteine protease.
TL;DR: Results of a secondary structure prediction algorithm suggest that the functional C‐terminal portion of AvrRpt2 is a cysteine protease whose activity is required for elimination of RIN4 during infection.
Journal ArticleDOI
Characterization of the Pseudomonas syringae pv. tomato AvrRpt2 protein: demonstration of secretion and processing during bacterial pathogenesis
TL;DR: Direct evidence is provided that mature AvrRpt2 protein is secreted from Pst DC3000 and that secretion is hrp dependent and it is shown that Avr rpt2 is N‐terminally processed when Arabidopsis thaliana plants are infected with PstDC3000 expressing avrRPT2.
References
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A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria
TL;DR: In this paper, a new vector strategy for the insertion of foreign genes into the genomes of gram negative bacteria not closely related to Escherichia coli was developed, which can utilize any gram negative bacterium as a recipient for conjugative DNA transfer.
Journal ArticleDOI
Green fluorescent protein as a marker for gene expression
TL;DR: A complementary DNA for the Aequorea victoria green fluorescent protein produces a fluorescent product when expressed in prokaryotic or eukaryotic cells, which can be used to monitor gene expression and protein localization in living organisms.