Journal ArticleDOI
Crystal structure of the periplasmic component of a tripartite macrolide-specific efflux pump.
Soohwan Yum,Yongbin Xu,Shunfu Piao,Se-Hoon Sim,Hong-Man Kim,Wol-Soon Jo,Kyung-Jin Kim,Hee-Seok Kweon,Min-Ho Jeong,Hyesung Jeon,Kangseok Lee,Nam-Chul Ha +11 more
TLDR
The crystal structure of E. coli MacA and the experimentally phased map of Actinobacillus actinomycetemcomitans MacA reveal a domain orientation of MacA different from that of AcrA, which provides insight into the oligomeric state in the functional complex of the drug efflux pump and type I secretion system.About:
This article is published in Journal of Molecular Biology.The article was published on 2009-04-17. It has received 106 citations till now. The article focuses on the topics: Periplasmic space & Bacterial outer membrane.read more
Citations
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Journal ArticleDOI
Molecular mechanisms of antibiotic resistance.
TL;DR: Recent advances in understanding of the mechanisms by which bacteria are either intrinsically resistant or acquire resistance to antibiotics are reviewed, including the prevention of access to drug targets, changes in the structure and protection of antibiotic targets and the direct modification or inactivation of antibiotics.
Journal ArticleDOI
The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria
TL;DR: This article highlights the recent progress obtained for organisms of clinical significance, together with methodological considerations for the characterization of MDR pumps, with particular focus on AcrAB-TolC and Mex pumps.
Journal ArticleDOI
Secretion systems in Gram-negative bacteria: structural and mechanistic insights
Tiago R. D. Costa,Catarina Felisberto-Rodrigues,Amit Meir,Marie S. Prevost,Adam Redzej,Martina Trokter,Gabriel Waksman +6 more
TL;DR: The structural and mechanistic relationships between these single- and double-membrane-embedded systems are explored, and how this knowledge can be exploited for the development of new antimicrobial strategies are discussed.
Journal ArticleDOI
Efflux-mediated drug resistance in bacteria: an update.
Xian-Zhi Li,Hiroshi Nikaido +1 more
TL;DR: The multifaceted implications of drug efflux transporters warrant novel strategies to combat multidrug resistance in bacteria.
Journal ArticleDOI
Robust and accurate prediction of residue-residue interactions across protein interfaces using evolutionary information.
TL;DR: It is found that residue pairs identified using a pseudo-likelihood-based method to covary across protein–protein interfaces in the 50S ribosomal unit and 28 additional bacterial protein complexes with known structure are almost always in contact in the complex.
References
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Book ChapterDOI
Processing of X-ray diffraction data collected in oscillation mode
Zbyszek Otwinowski,Wladek Minor +1 more
TL;DR: The methods presented in the chapter have been applied to solve a large variety of problems, from inorganic molecules with 5 A unit cell to rotavirus of 700 A diameters crystallized in 700 × 1000 × 1400 A cell.
Journal ArticleDOI
Coot: model-building tools for molecular graphics.
Paul Emsley,Kevin Cowtan +1 more
TL;DR: CCP4mg is a project that aims to provide a general-purpose tool for structural biologists, providing tools for X-ray structure solution, structure comparison and analysis, and publication-quality graphics.
Journal ArticleDOI
The CCP4 suite: programs for protein crystallography
TL;DR: The CCP4 (Collaborative Computational Project, number 4) program suite is a collection of programs and associated data and subroutine libraries which can be used for macromolecular structure determination by X-ray crystallography.
Journal ArticleDOI
Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination
Axel T. Brunger,Axel T. Brunger,Paul D. Adams,G M Clore,W. L. DeLano,Piet Gros,R.W. Grosse-Kunstleve,R.W. Grosse-Kunstleve,Jiansheng Jiang,J. Kuszewski,Michael Nilges,Navraj S. Pannu,Randy J. Read,Luke M. Rice,Thomas Simonson,Gregory L. Warren +15 more
TL;DR: The Crystallography & NMR System (CNS) as mentioned in this paper is a software suite for macromolecular structure determination by X-ray crystallography or solution nuclear magnetic resonance (NMR) spectroscopy.
Journal ArticleDOI
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.