DNAzyme-based probes for telomerase detection in early-stage cancer diagnosis.
Hui Wang,Michael J. Donovan,Ling Meng,Zilong Zhao,Zilong Zhao,Youngmi Kim,Mao Ye,Weihong Tan,Weihong Tan +8 more
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TLDR
A DNAzyme-based probe to detect the presence of telomerase in cell lysates with a fivefold increase in signal level for cancer cells over normal cells is described.Abstract:
Human telomerase is a polymerase enzyme that adds tandem repeats of DNA (TTAGGG) in the telomeric region to the ends of chromosomes. Since telomerase can be detected in immortalized, but not normal, somatic cells, it has been considered a selective target for cancer chemotherapy. Here, we describe a DNAzyme-based probe to detect the presence of telomerase in cell lysates. Telomerase elongates the primer site on the probe. Subsequent addition of the Pb(II) cofactor activates the DNAzyme, which cleaves the elongated fragment at the RNA site, releasing the probe for repetitive cycling and signal amplification. The cleaved fragment is detected by a reporter molecular beacon. Enzymatic amplification with rapid turnover allows detection of telomerase in the range of 0.1 to 1 μg cell lysate, with a fivefold increase in signal level for cancer cells over normal cells. This probe design can provide a simple, yet rapid and sensitive, measurement of telomerase activity.read more
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References
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Journal Article
Telomerase Activity in Human Liver Tissues: Comparison between Chronic Liver Disease and Hepatocellular Carcinomas
Hidetoshi Tahara,Toshio Nakanishi,Mikiya Kitamoto,R. Nakashio,Jerry W. Shay,Eiichi Tahara,Goro Kajiyama,Toshinori Ide +7 more
TL;DR: Telomerase activity was examined in 105 frozen samples from human normal liver tissues, chronic liver disease, and hepatocellular carcinoma to indicate that the expression of telomerase may play a crucial role in hepatocarcinogenesis.
Journal ArticleDOI
Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol
TL;DR: Cost-effective procedures for detection of telomerase activity using a fluorescent-based assay as well as by using real-time PCR are described.
Journal Article
Telomerase activity in preneoplastic and neoplastic gastric and colorectal lesions.
Hidetoshi Tahara,Hiroki Kuniyasu,Hiroshi Yokozaki,Wataru Yasui,Jerry W. Shay,Toshinori Ide,Eiichi Tahara +6 more
TL;DR: Results indicate overall that reactivation of telomerase may occur at an early stage of carcinogenesis and may correlate well with malignant progression of gastric cancer, and may serve as a powerful additional tool for cancer diagnosis.
Journal ArticleDOI
Detection of Telomerase Activity in Exfoliated Cells in Urine From Patients With Bladder Cancer
Hidefumi Kinoshita,Osamu Ogawa,Yoshiyuki Kakehi,Mutsuki Mishina,Noriyuki Itoh,Hitoshi Yamada,Toshiro Terachi,Osamu Yoshida +7 more
TL;DR: Telomerase activity can be detected in exfoliated cells in urine from patients with bladder cancer, and measurement of this activity appears to be more sensitive in detecting the presence of cancer than standard urine cytologic examination.
Journal Article
Telomerase activity in human bladder cancer
Yi Lin,Hiroshi Miyamoto,Kiyoshi Fujinami,Hiroji Uemura,Masahiko Hosaka,Yoshiko Iwasaki,Yoshinobu Kubota +6 more
TL;DR: Telomerase activity might be suggested to represent an additional required event in the multigenetic process of tumorigenesis in human bladder cancer.
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