Efficient generation of transgenic pigs using equine infectious anaemia virus (EIAV) derived vector.
C. Bruce A. Whitelaw,Pippa A Radcliffe,W. A. Ritchie,Ailsa J Carlisle,F M Ellard,Romi N. Pena,Jo M. Rowe,A. John Clark,Tim King,Kyriacos A. Mitrophanous +9 more
TLDR
Efficient generation of transgenic pigs using an equine infectious anaemia virus derived vector is demonstrated, indicating that lentiviral transgene delivery may be a general tool with which to efficiently generate transgenic mammals.About:
This article is published in FEBS Letters.The article was published on 2004-07-30 and is currently open access. It has received 143 citations till now. The article focuses on the topics: Transgene.read more
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Gene delivery by lentivirus vectors
Adam S. Cockrell,Tal Kafri +1 more
TL;DR: State of the art technology for lentiviral vector production by transient transfection and packaging cell lines are explicitly presented with current practices used for concentration, purification, titering, and determining the safety of a vector stock.
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Transgenic pigs as models for translational biomedical research
Bernhard Aigner,Simone Renner,Barbara Kessler,Nikolai Klymiuk,Mayuko Kurome,Annegret Wünsch,Eckhard Wolf +6 more
TL;DR: An overview of the current techniques for genetic modification of pigs and the transgenic pig models established for neurodegenerative diseases, cardiovascular diseases, cystic fibrosis, and diabetes mellitus is provided.
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Oncogenesis following delivery of a nonprimate lentiviral gene therapy vector to fetal and neonatal mice.
Michael Themis,Simon N. Waddington,Manfred Schmidt,Christof von Kalle,Christof von Kalle,Yoahe Wang,Faisal A. Al-Allaf,Lisa G. Gregory,M Nivsarkar,M Themis,Maxine V. Holder,Suzanne M. K. Buckley,Niraja Dighe,Alaine Ruthe,A Mistry,Brian W. Bigger,Ahad A. Rahim,Tuan H. Nguyen,Didier Trono,Adrian J. Thrasher,Charles Coutelle +20 more
TL;DR: A high incidence of lentiviral vector-associated tumorigenesis following in utero and neonatal gene transfer in mice may provide a highly sensitive model to investigate integrating vector safety prior to clinical application.
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Lentivirus-Mediated Gene Transfer to the Central Nervous System: Therapeutic and Research Applications
Liang-Fong Wong,Lucy Goodhead,Christine Prat,Kyriacos A. Mitrophanous,Susan M. Kingsman,Nicholas D. Mazarakis +5 more
TL;DR: The applications of lentiviral vectors in therapy and research are discussed and the essential features that will ensure their translation to the clinic in the near future are highlighted.
Journal Article
Oncogenesis following delivery of a nonprimate lentiviral gene therapy vector to fetal and neonatal mice (vol 12, pg 763, 2005)
Michael Themis,Simon N. Waddington,Manfred G. Schmidt,C. von Kalle,Yaohe Wang,Faisal A. Al-Allaf,Lisa G. Gregory,M Nivsarkar,Maxine V. Holder,Smk Buckley,Niraja Dighe,Alaine Ruthe,A Mistry,Brian W. Bigger,AA Rahim,Tuan H. Nguyen,Didier Trono,Adrian J. Thrasher,Charles Coutelle +18 more
References
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Viable offspring derived from fetal and adult mammalian cells
TL;DR: The birth of lambs from differentiated fetal and adult cells confirms that differentiation of that cell did not involve the irreversible modification of genetic material required for development to term and reinforces previous speculation that by inducing donor cells to become quiescent it will be possible to obtain normal development from a wide variety of differentiated cells.
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Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral Vectors
TL;DR: Transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter are generated and transgenic rats that express GFP at high levels are generated, suggesting that this technique can be used to produce other transgenic animal species.
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A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference
Douglas A. Rubinson,Christopher P. Dillon,Adam V. Kwiatkowski,Claudia Sievers,Claudia Sievers,Lili Yang,Johnny E. Kopinja,Dina L Rooney,Mingdi Zhang,Melanie Ihrig,Michael T. McManus,Frank B. Gertler,Martin L. Scott,Luk Van Parijs +13 more
TL;DR: It is shown that lentivirus-delivered shRNAs are capable of specific, highly stable and functional silencing of gene expression in a variety of cell types and also in transgenic mice.
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Production of transgenic rabbits, sheep and pigs by microinjection
Robert E. Hammer,Vernon G. Pursel,Caird E. Rexroad,Robert J. Wall,D. J. Bolt,Karl M. Ebert,Richard D. Palmiter,Ralph L. Brinster +7 more
TL;DR: Integration of the fusion gene in all three species and expression of the gene in transgenic rabbits and pigs are reported here.
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Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts
Angelika Schnieke,Alexander Kind,W. A. Ritchie,Karen Mycock,A Scott,Marjorie Ritchie,Ian Wilmut,Alan Colman,Keith H.S. Campbell +8 more
TL;DR: Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk and produced viable animals by nuclear transfer.