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Journal ArticleDOI

Electron tomography and holography in materials science

TLDR
An overview of the techniques of electron tomography and electron holography is presented and their capabilities with the aid of case studies that span materials science and the interface between the physical sciences and the life sciences are demonstrated.
Abstract
The rapid development of electron tomography, in particular the introduction of novel tomographic imaging modes, has led to the visualization and analysis of three-dimensional structural and chemical information from materials at the nanometre level. In addition, the phase information revealed in electron holograms allows electrostatic and magnetic potentials to be mapped quantitatively with high spatial resolution and, when combined with tomography, in three dimensions. Here we present an overview of the techniques of electron tomography and electron holography and demonstrate their capabilities with the aid of case studies that span materials science and the interface between the physical sciences and the life sciences.

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Citations
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Journal ArticleDOI

Improving reproducibility in synchrotron tomography using implementation-adapted filters

TL;DR: In this article, the authors proposed a method to reduce the differences between reconstructed images obtained from different software packages by optimizing the filter used in analytical algorithms, which can be computed using a wrapper routine around a black-box implementation of a reconstruction algorithm, leading to quantitatively similar reconstructions.

Single-shot, coherent, pop-out 3D metrology

TL;DR: In this article , a coherent imaging alternative was proposed to recover the 3D volume of a thin, homogeneously amorphous specimen with only a single, energy-filtered, bright-field image.
Journal ArticleDOI

Improving reproducibility in synchrotron tomography using implementation-adapted filters.

TL;DR: In this paper, the authors proposed a method to reduce the differences between reconstructed images and corresponding segmentations by optimizing the filter used in analytical algorithms, which can be computed using a wrapper routine around a black-box implementation of a reconstruction algorithm.
Posted Content

Distributed Reconstruction Algorithm for Electron Tomography with Multiple-scattering Samples

TL;DR: In this article, a distributed computing framework is proposed to reconstruct large volumes by decomposing a projected tilt-series into smaller datasets such that sub-volumes can be simultaneously reconstructed on separate compute nodes using a cluster.
References
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Journal ArticleDOI

Representation of a Function by Its Line Integrals, with Some Radiological Applications

TL;DR: In this paper, a method for finding a real function in a finite region of a plane given its line integrals along all straight lines intersecting the region was given, and the solution found is applicable to three problems of interest for precise radiology and radiotherapy: (1) the determination of a variable x-ray absorption coefficient in two dimensions, (2) the distribution of positron annihilations when there is an inhomogeneous distribution of the positron emitter in matter, and (3) a variable density of matter with constant chemical composition, using the energy loss
Journal ArticleDOI

Iterative methods for the three-dimensional reconstruction of an object from projections

TL;DR: It is shown that in general ART produces erroneous reconstructions, and an alternative iterative method is proposed which will give correct reconstructions under certain conditions.
Journal ArticleDOI

Reconstruction of Three Dimensional Structures from Electron Micrographs

TL;DR: General principles are formulated for the objective reconstruction of a three dimensional object from a set of electron microscope images and applied to the calculation of aThree dimensional density map of the tail of bacteriophage T4.
MonographDOI

Three-Dimensional Electron Microscopy of Macromolecular Assemblies

Joachim Frank
TL;DR: In this article, a three-dimensional reconstruction interpretation of 3D images of macromolecules is presented. Butts et al. reconstructed macromoles from micrographs of single macromolcules.
Journal ArticleDOI

Molecular structure determination by electron microscopy of unstained crystalline specimens.

TL;DR: The projected structures of two unstained periodic biological specimens, the purple membrane and catalase, have been determined by electron microscopy to resolutions of 7 A and 9 A, respectively.
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