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Journal ArticleDOI

Electron tomography and holography in materials science

TLDR
An overview of the techniques of electron tomography and electron holography is presented and their capabilities with the aid of case studies that span materials science and the interface between the physical sciences and the life sciences are demonstrated.
Abstract
The rapid development of electron tomography, in particular the introduction of novel tomographic imaging modes, has led to the visualization and analysis of three-dimensional structural and chemical information from materials at the nanometre level. In addition, the phase information revealed in electron holograms allows electrostatic and magnetic potentials to be mapped quantitatively with high spatial resolution and, when combined with tomography, in three dimensions. Here we present an overview of the techniques of electron tomography and electron holography and demonstrate their capabilities with the aid of case studies that span materials science and the interface between the physical sciences and the life sciences.

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Citations
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Journal ArticleDOI

State of the art in atomic resolution off-axis electron holography

TL;DR: In this article, a high-brightness Schottky field electron emitter in a state-of-the-art transmission electron microscope operated at 300kV was used to enhance phase signal resolution at atomic resolution.
Journal ArticleDOI

Crystal growth of bullet-shaped magnetite in magnetotactic bacteria of the Nitrospirae phylum

TL;DR: A comprehensive transmission electron microscopy (TEM) study of magnetic and structural properties down to atomic scales on bullet-shaped magnetites produced by the uncultured strain MYR-1 belonging to the Nitrospirae phylum, a deeply branching phylogenetic MTB group.
Book ChapterDOI

1.14 – Magnetic Nanoparticles

TL;DR: In this article, the magnetic properties of nanoparticles are reviewed with a focus on both theoretical work and how the dynamics can be experimentally observed, including the effects of dipole interactions between ferromagnetic particles and exchange interactions between antiferromagnetic nanoparticles.
Journal ArticleDOI

Refinement procedure for the image alignment in high-resolution electron tomography

TL;DR: A tomographic-reconstruction based and marker-free method is proposed, which uses an iterative optimisation of the tomogram resolution to provide the required correlation over a large tilt angle separation and guarantees a consistent alignment of images for the full range of object tilt angles.
References
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Journal ArticleDOI

Representation of a Function by Its Line Integrals, with Some Radiological Applications

TL;DR: In this paper, a method for finding a real function in a finite region of a plane given its line integrals along all straight lines intersecting the region was given, and the solution found is applicable to three problems of interest for precise radiology and radiotherapy: (1) the determination of a variable x-ray absorption coefficient in two dimensions, (2) the distribution of positron annihilations when there is an inhomogeneous distribution of the positron emitter in matter, and (3) a variable density of matter with constant chemical composition, using the energy loss
Journal ArticleDOI

Iterative methods for the three-dimensional reconstruction of an object from projections

TL;DR: It is shown that in general ART produces erroneous reconstructions, and an alternative iterative method is proposed which will give correct reconstructions under certain conditions.
Journal ArticleDOI

Reconstruction of Three Dimensional Structures from Electron Micrographs

TL;DR: General principles are formulated for the objective reconstruction of a three dimensional object from a set of electron microscope images and applied to the calculation of aThree dimensional density map of the tail of bacteriophage T4.
MonographDOI

Three-Dimensional Electron Microscopy of Macromolecular Assemblies

Joachim Frank
TL;DR: In this article, a three-dimensional reconstruction interpretation of 3D images of macromolecules is presented. Butts et al. reconstructed macromoles from micrographs of single macromolcules.
Journal ArticleDOI

Molecular structure determination by electron microscopy of unstained crystalline specimens.

TL;DR: The projected structures of two unstained periodic biological specimens, the purple membrane and catalase, have been determined by electron microscopy to resolutions of 7 A and 9 A, respectively.
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