Elucidation and Identification of Double-Tip Effects in Atomic Force Microscopy Studies of Biological Structures
TLDR
The results can serve as a foundation to design computer-based automatic detection of double-tip AFM images during nanoscale measuring and imaging of biomolecules and even non-biological materials or structures, and then personal experience is not needed any longer to evaluate artifactual images induced by the double- Tip/probe effect.Abstract:
While atomic force microscopy (AFM) has been increasingly applied to life science, artifactual measurements or images can occur during nanoscale analyses of cell components and biomolecules. Tip-sample convolution effect is the most common mechanism responsible for causing artifacts. Some deconvolution-based methods or algorithms have been developed to reconstruct the specimen surface or the tip geometry. Double-tip or double-probe effect can also induce artifactual images by a different mechanism from that of convolution effect. However, an objective method for identifying the double-tip/probe-induced artifactual images is still absent. To fill this important gap, we made use of our expertise of AFM to analyze artifactual double-tip images of cell structures and biomolecules, such as linear DNA, during AFM scanning and imaging. Mathematical models were then generated to elucidate the artifactual double-tip effects and images develop during AFM imaging of cell structures and biomolecules. Based on these models, computational formulas were created to measure and identify potential double-tip AFM images. Such formulas proved to be useful for identification of double-tip images of cell structures and DNA molecules. The present studies provide a useful methodology to evaluate double-tip effects and images. Our results can serve as a foundation to design computer-based automatic detection of double-tip AFM images during nanoscale measuring and imaging of biomolecules and even non-biological materials or structures, and then personal experience is not needed any longer to evaluate artifactual images induced by the double-tip/probe effect.read more
Citations
More filters
Journal ArticleDOI
Effects of long-term serial cell passaging on cell spreading, migration, and cell-surface ultrastructures of cultured vascular endothelial cells
TL;DR: It is found that the abilities of cell spreading and migration first increased at early passages and then decreased after passage 15, in agreement with the changes in average length of actin filaments, which implies that for pre-stored adherent cells at −80 °C cell passages 5–10 are optimal for in vitro studies.
Journal ArticleDOI
Double-Tip Artifact Removal From Atomic Force Microscopy Images
Yun-feng Wang,Jason I. Kilpatrick,Suzanne P. Jarvis,Francis M. Boland,Anil Kokaram,David Corrigan +5 more
TL;DR: This work applies a novel deblurring technique, using a Bayesian framework, to yield a reliable estimation of the real surface topography without any prior knowledge of the tip geometry (blind reconstruction), and focuses specifically on the double-tip effect.
Journal ArticleDOI
End-to-end differentiable blind tip reconstruction for noisy atomic force microscopy images
TL;DR: Differentiable blind tip reconstruction (DTR) as mentioned in this paper is an alternative to the BTR algorithm that estimates tip shape only from high-speed atomic force microscopy images using mathematical morphology operators.
Journal ArticleDOI
Spatiotemporal resolution in high-speed atomic force microscopy for studying biological macromolecules in action
TL;DR: In this paper , the authors explain the principle of high-speed atomic force microscopy (HS-AFM) and describe how the resolution is determined, and discuss recent attempts to improve the resolution of HS-AFMs to further extend the observable range of biological phenomena.
References
More filters
Journal ArticleDOI
Fingerprinting polysaccharides with single-molecule atomic force microscopy.
TL;DR: The use of an atomic force microscopy (AFM)-based force spectroscopy technique to identify, at the single-molecule level, the components of mixtures of polysaccharides is reported, an important addition to the arsenal of analytical techniques used in carbohydrate research.
Journal ArticleDOI
Tip artefacts in scanning force microscopy
TL;DR: In this paper, a brief description of the preparation and characteristics of the most commonly used scanning force microscopy (SFM) tips is given and a variety of different artefacts originating from tip properties is presented and illustrated with selected scanning force micrographs.
Journal ArticleDOI
Quantitative characterization of biomolecular assemblies and interactions using atomic force microscopy
TL;DR: This review focuses on the application of AFM for quantitatively characterizing the structural and thermodynamic properties of protein-protein and protein-nucleic acid complexes.
Journal ArticleDOI
Tip artifacts in atomic force microscope imaging of thin film surfaces
TL;DR: In this article, a study of tip artifacts in atomic force microscope (AFM) images of thin film surfaces was conducted, and the authors found that for a significant fraction of the images, the AFM image was affected by this type of tip artifact.
Journal ArticleDOI
Tip artifacts of microfabricated force sensors for atomic force microscopy
TL;DR: In this paper, it was demonstrated that due to inevitable intrinsic imperfections in the microfabrication process of atomic force microscopy (AFM) tips, images of rough surfaces can be totally dominated by tip artifacts.