Emerging nanolabels-based immunoassays: principle and applications in food safety
Ruiyuan Pan,Guoliang Li,Shucheng Liu,Xianlong Zhang,Jianghua Liu,Zhuoqun Su,Yongning Wu,Yongning Wu +7 more
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In this paper, the authors summarize the advances of nanolabels in immunoassays, covering nanomaterials as nanozymes, direct signal indicators, enzyme carriers, etc.Abstract:
Food safety issues pose a serious threat to human health, and appropriate detection technologies can effectively ensure food safety. Immunoassay is the gold standard for highly sensitive detection of biochemical targets, providing a good platform for food contaminants detection. In immunoassay, the antibody or antigen conjugated labels are the most important units because they play roles to convert the information of target analytes to easily detected signal. Recently, various efforts have been paid into the label design to enhance the detection sensitivity and stability. Nanomaterials own advantages such as facile preparation, excellent stability, low cost and multi-category signal output, showing great potential as novel labels. Herein, we summarize the advances of nanolabels in immunoassays, covering nanomaterials as nanozymes, direct signal indicators, enzyme carriers, etc. Moreover, applications of nanolabels-based immunoassays in the detection of food contaminants (pathogenic microorganisms, allergic proteins, biotoxins, and others) are comprehensively concluded. Finally, challenges and perspectives on the future trends are also proposed.read more
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References
More filters
Journal ArticleDOI
Global trends in emerging infectious diseases
Kate E. Jones,Nikkita Gunvant Patel,Marc A. Levy,Adam Storeygard,Adam Storeygard,Deborah Balk,Deborah Balk,John L. Gittleman,Peter Daszak +8 more
TL;DR: It is concluded that global resources to counter disease emergence are poorly allocated, with the majority of the scientific and surveillance effort focused on countries from where the next important EID is least likely to originate.
Journal ArticleDOI
Intrinsic peroxidase-like activity of ferromagnetic nanoparticles
Lizeng Gao,Jie Zhuang,Leng Nie,Jinbin Zhang,Yu Zhang,Ning Gu,Taihong Wang,Jing Feng,Dongling Yang,Sarah Perrett,Xiyun Yan +10 more
TL;DR: It is reported that magnetite nanoparticles in fact possess an intrinsic enzyme mimetic activity similar to that found in natural peroxidases, which are widely used to oxidize organic substrates in the treatment of wastewater or as detection tools.
Journal ArticleDOI
Nanomaterials with enzyme-like characteristics (nanozymes): next-generation artificial enzymes
Hui Wei,Erkang Wang +1 more
TL;DR: This review discusses various nanomaterials that have been explored to mimic different kinds of enzymes and covers their kinetics, mechanisms and applications in numerous fields, from biosensing and immunoassays, to stem cell growth and pollutant removal.
Journal ArticleDOI
Graphene oxide: intrinsic peroxidase catalytic activity and its application to glucose detection.
TL;DR: A simple, cheap, and highly sensitive and selective colorimetric method for glucose detection has been developed and will facilitate the utilization of GO-COOH intrinsic peroxidase activity in medical diagnostics and biotechnology.
Journal ArticleDOI
Electrochemical Sensors and Biosensors Based on Nanomaterials and Nanostructures
TL;DR: New advances in electrochemical sensors and biosensors based on nanomaterials and nanostructures during 2013 to 2014 are focused on to provide the reader with a clear and concise view of new advances in areas ranging from electrode engineering, strategies for electrochemical signal amplification, and novel electroanalytical techniques used in the miniaturization and integration of the sensors.