Journal ArticleDOI
Exploring Ion Suppression in Mass Spectrometry Imaging of a Heterogeneous Tissue
TLDR
This study explored several aspects of regional analyte suppression in mass spectrometry imaging (MSI) of a heterogeneous sample, transverse cryosections of mouse brain and employed the concept of a tissue extinction coefficient (TEC) to assess suppression of an analyte on tissue relative to its intensity in an off tissue region.Abstract:
In this study we have explored several aspects of regional analyte suppression in mass spectrometry imaging (MSI) of a heterogeneous sample, transverse cryosections of mouse brain. Olanzapine was homogeneously coated across the section prior to desorption electrospray ionization (DESI) and matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging. We employed the concept of a tissue extinction coefficient (TEC) to assess suppression of an analyte on tissue relative to its intensity in an off tissue region. We expanded the use of TEC, by first segmenting anatomical regions using graph-cuts clustering and calculating a TEC for each cluster. The single ion image of the olanzapine [M + H]+ ion was seen to vary considerably across the image, with anatomical features such as the white matter and hippocampus visible. While trends in regional ion suppression were conserved across MSI modalities, significant changes in the magnitude of relative regional suppression effects between techniques we...read more
Citations
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Journal ArticleDOI
Ambient ionization Mass Spectrometry: Recent Developments and Applications
TL;DR: In the last 15 years, innovations in the field of ambient ionization MS have grown expansively, pushing these technologies far past their point of conception and integrating them into the broader scientific community in creative and stimulating ways.
Journal ArticleDOI
Advanced MALDI mass spectrometry imaging in pharmaceutical research and drug development.
TL;DR: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) has emerged as a key technology for label-free bioanalysis of the spatial distribution of biomolecules, pharmaceuticals and other xenobiotics in tissue sections.
Journal ArticleDOI
Spatially Resolved Mass Spectrometry at the Single Cell: Recent Innovations in Proteomics and Metabolomics.
TL;DR: In this article, the authors highlight important aspects related to high-throughput screening, data analysis, and more which are vital to the success of achieving proteomic and metabolomic profiling at the single cell scale.
Journal ArticleDOI
Reactive Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Using an Intrinsically Photoreactive Paternò–Büchi Matrix for Double-Bond Localization in Isomeric Phospholipids
TL;DR: Benzophenone (BPh) is introduced as a novel reactive matrix for matrix-assisted laser desorption/ionization (MALDI) and high lateral resolution MSI results of DB-position isomers are presented, highlighting the capabilities of BPh as a PB-reactive MALDI matrix to potentially unveil the impact ofDB- position isomers in PL metabolism.
Journal ArticleDOI
Spatial Metabolomics and Imaging Mass Spectrometry in the Age of Artificial Intelligence
TL;DR: This work introduces spatial metabolomics through the eyes of a computational scientist, review the outstanding challenges, provide a look into the future, and discuss opportunities granted by the ongoing convergence of human and artificial intelligence.
References
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Isotropic Fractionator: A Simple, Rapid Method for the Quantification of Total Cell and Neuron Numbers in the Brain
TL;DR: A novel, fast, and inexpensive method to quantify total numbers of neuronal and non-neuronal cells in the brain or any dissectable regions thereof, which consists of transforming highly anisotropic brain structures into homogeneous, isotropic suspensions of cell nuclei, which can be counted and identified immunocytochemically as neuronal or non-NEuronal.
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Cellular scaling rules for rodent brains
TL;DR: It is proposed that the faster increase in average neuronal size in the cerebral cortex than in the cerebellum as these structures gain neurons and the rapidly increasing glial numbers that generate glial mass to match total neuronal mass at a fixed glia/neuron total mass ratio are fundamental cellular constraints that lead to the relative expansion of cerebral cortical volume across species.