Journal ArticleDOI
Expression of lysosome-associated membrane protein 1 (Lamp-1) and galectins in human keratinocytes is regulated by differentiation
TLDR
The results suggest that the upregulated Lamp-1 expression at confluence could be involved in keratinocyte differentiation, but apparently not through interaction with galectin-3.Abstract:
Lysosomes and their components are suspected to be involved in epidermal differentiation. In this study, lysosomal enzyme activities, expression of the lysosome-associated membrane protein 1 (Lamp-1) and expression of the epidermal galectins-1, -3 and -7 were investigated in human keratinocytes cultured at different cell densities (subconfluence, confluence and postconfluence) in order to induce differentiation. Detected by Western blot and immunofluorescence, Lamp-1 expression is transiently upregulated at culture confluence, but reduced at postconfluence. Northern blot analyses performed on subconfluent, confluent and post-confluent cultures of keratinocytes show that Lamp-1 mRNA expression is also upregulated at culture confluence, but downregulated at postconfluence. Measurements of lysosomal enzyme activities indicate a transient upregulation at culture confluence, whereas cathepsins B, C and L are particularly downregulated at postconfluence. Cell density and differentiation of epidermal cells also differentially regulates galectin expression in autocrine cultures. As the expression of galectin-1 mRNA is high in subconfluent cells, it is assumed to be associated with their proliferative state. On the other hand, as the mRNA levels for galectins-3 and -7 are notably upregulated at culture confluence (galectin-7) or at postconfluence (galectin-3), their expression is thought to be related to the differentiated state of keratinocytes. However, we collected evidence by confocal microscopy that galectin-3 and Lamp-1 do not colocalize in vitro in keratinocytes. Altogether, our results suggest that the upregulated Lamp-1 expression at confluence could be involved in keratinocyte differentiation, but apparently not through interaction with galectin-3.read more
Citations
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Journal ArticleDOI
Alterations in lysosomal and proteasomal markers in Parkinson's disease: relationship to alpha-synuclein inclusions.
TL;DR: It is suggested that alpha-syn aggregation is a key feature associated with decline of proteasome and lysosome and support the hypothesis that cell degeneration in PD involves proteosomal andlysosomal dysfunction, impaired protein clearance, and protein accumulation and aggregation leading to cell death.
Journal ArticleDOI
Galectin-3 and the skin.
TL;DR: Galectin-3 may play an important role in tumor cell growth, apoptosis, cell motility, invasion, and metastasis, and may be a novel therapeutic target for a variety of skin diseases.
Journal ArticleDOI
Deimination is regulated at multiple levels including auto-deimination of peptidylarginine deiminases
Marie-Claire Méchin,Fanny Coudane,Véronique Adoue,Jacques Arnaud,Hélène Duplan,Marie Charveron,Anne-Marie Schmitt,Hidenari Takahara,Guy Serre,Michel Simon +9 more
TL;DR: Deimination can be regulated at multiple levels: transcription of the PADI genes, translation of the corresponding mRNAs, and auto-deimination of PADs.
Journal ArticleDOI
Human skin cell stress response to GSM-900 mobile phone signals. In vitro study on isolated primary cells and reconstructed epidermis.
Sandrine Sanchez,Alexandra Milochau,Gilles Ruffié,Florence Poulletier de Gannes,Isabelle Lagroye,Emmanuelle Haro,Jean-Etienne Surleve-Bazeille,Bernard Billaudel,Maguy Lassegues,Bernard Veyret +9 more
TL;DR: The results suggest that adaptive cell behaviour in response to RFR exposure, depending on the cell type and culture conditions, is unlikely to have deleterious effects at the skin level.
Journal ArticleDOI
Galectin-7 in Epithelial Homeostasis and Carcinomas
TL;DR: The physiological functions of galectin-7 in epithelia are focused on and the alterations of galector-7 expression in carcinomas are presented with the aim of describing its possible functions in tumour progression.
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Galectins: versatile modulators of cell adhesion, cell proliferation, and cell death
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Journal ArticleDOI
Galectins and their ligands: amplifiers, silencers or tuners of the inflammatory response?
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TL;DR: The current wealth of new information promises a future scenario in which individual members of the galectin family or their ligands will be used as powerful anti-inflammatory mediators and selective modulators of the immune response.