Journal ArticleDOI
Expression of the γ-δ T-cell receptor on intestinal CD8+ intraepithelial lymphocytes
Thomas Goodman,Leo Lefrançois +1 more
TLDR
It is reported that most mature T cells residing in the murine intestinal epithelium express CD3-associated TCRs composed of γ-chains distilphide-linked to a protein resembling the δ-chain.Abstract:
The vast majority of mature T lymphocytes in the peripheral blood and lymphoid organs use the CD3-associated alpha, beta T-cell receptor (TCR) heterodimer for antigen recognition. A second class of TCRs consists of disulphide-linked gamma and delta proteins that are also CD3-associated. A subset of early CD3+ fetal and adult CD4- 8- thymocytes express gamma, delta TCRs before alpha, beta TCRs are detectable. In addition, a minor (1-5%) subpopulation of peripheral T lymphocytes, and some spleen cells from nude mice express gamma, delta TCRs. Notably, dendritic epidermal cells have also been shown to express gamma, delta TCRs. All of these populations lack CD4 and CD8 molecules. We now report that most mature T cells residing in the murine intestinal epithelium express CD3-associated TCRs composed of gamma-chains disulphide-linked to a protein resembling the delta-chain. The striking feature of these intraepithelial lymphocytes (IEL) was that they were exclusively CD4-8+. In addition, approximately half of CD3-bearing IEL lacked detectable Thy-1 on the cell surface, which is unprecedented for murine T cells. In contrast to other CD8+ peripheral T cells, freshly isolated IEL could be induced to display cytolytic activity by engaging the CD3 molecule, indicating that activation had occurred in vivo. Thus, CD8+ IEL are a phenotypically diverse and anatomically restricted population of lymphocytes that use gamma-chain containing heterodimers for antigen recognition.read more
Citations
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Journal ArticleDOI
The immune system. First of two parts.
Peter J. Delves,Ivan M. Roitt +1 more
TL;DR: The immune system is an organization of cells and molecules with specialized roles in defending against infection as discussed by the authors, and there are two fundamentally different types of responses to invading microbes: innate and adaptive.
Journal ArticleDOI
[gamma][delta] cells: a right time and a right place for a conserved third way of protection.
TL;DR: The properties of g Mohammadelta cells form a basis for understanding gammadelta cell interactions with antigens and other cells that in turn form a based for understanding immunoprotective and regulatory functions of gammad delta cells in vivo.
Book ChapterDOI
The Immune System
TL;DR: In this article, a mouse is injected subcutaneously with a suspension of appropriate bacteria, and either the mouse falls ill and succumbs to the infection within a few days, or it recovers.
Journal ArticleDOI
The mucosal immune system : from fundamental concepts to vaccine development
Jerry R. McGhee,Jiri Mestecky,Mark T. Dertzbaugh,John H. Eldridge,Masatomo Hirasawa,Hiroshi Kiyono +5 more
TL;DR: Practical approaches for vaccine construction and delivery into mucosal inductive sites in an effort to elicit host protection at mucosal surfaces where the infection actually occurs are proposed.
Journal ArticleDOI
Modulation of epithelial cell growth by intraepithelial gamma delta T cells
Richard Boismenu,Wendy L. Havran +1 more
TL;DR: It is shown here that activated gamma delta T cells obtained from skin and intestine express the epithelial cell mitogen keratinocyte growth factor (KGF), which suggests that intraepithelial alpha beta T cells function in surveillance and in repair of damaged epithelial tissues.
References
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Identification of a monoclonal antibody specific for a murine T3 polypeptide
TL;DR: Results identify T3-epsilon as a cell surface protein involved in the transduction of activation signals and can both activate and inhibit T-cell function.
Journal ArticleDOI
"Panning" for lymphocytes: a method for cell selection
L J Wysocki,V L Sato +1 more
TL;DR: The plastic dish method will be generally useful for fractionating cells on the basis of their cell surface antigens and can be modified to allow the selection of cells by a double-antibody procedure.
Journal ArticleDOI
Identification of a putative second T-cell receptor.
Michael B. Brenner,Michael B. Brenner,Joanne McLean,Deno P. Dialynas,Jack L. Strominger,John A. Smith,Frances L. Owen,Jonathan G. Seidman,Stephen C.Y. Ip,Fred S. Rosen,Michael S. Krangel +10 more
TL;DR: Chemical crosslinking experiments reveal that these lymphocytes express novel T3-associated polypeptides, one of which appears to be the product of the Tγ gene.
Journal ArticleDOI
Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril.
Markwell Ma,Fox Cf +1 more
TL;DR: The effectiveness, simplicity of use, and versatility of chloroglycoluril recommend it as an iodinating reagent for both surface-specific and nonvectorial labeling of membrane systems.
Journal ArticleDOI
Hybrid antibodies can target sites for attack by T cells
TL;DR: It is shown that heteroconjugates of monoclonal antibodies (referred to hereafter as hybrid antibodies), in which one of the component binding sites is anti-T-cell receptor and the other component binding site is directed against any chosen target antigen, can focus T cells to act at the targeted site.
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