Flow cytometric detection of dyserythropoiesis: a sensitive and powerful diagnostic tool for myelodysplastic syndromes.
Stéphanie Mathis,Nicolas Chapuis,Camille Debord,Alexandra Rouquette,Isabelle Radford-Weiss,Sophie Park,Francois Dreyfus,Catherine Lacombe,M C Béné,Olivier Kosmider,Michaela Fontenay,Valérie Bardet +11 more
TLDR
The aim of this study was to identify markers of dyserythropoiesis relevant for the diagnosis of MDS analyzed by selecting erythroblasts in a whole no-lysis bone marrow strategy by using a nuclear dye.Abstract:
Several groups have published flow cytometry scores useful for the diagnosis or prognosis of myelodysplastic syndromes (MDS), mainly based on the detection of immunophenotypic abnormalities in the maturation of granulocytic/monocytic and lymphoid lineages. As anemia is the most frequent symptom of early MDS, the aim of this study was to identify markers of dyserythropoiesis relevant for the diagnosis of MDS analyzed by selecting erythroblasts in a whole no-lysis bone marrow strategy by using a nuclear dye. This prospective study included 163 patients, including 126 with cytopenias leading to MDS suspicion and 46 controls without MDS. In a learning cohort of 53 unequivocal MDS with specific markers, there was a significant difference between the coefficients of variation of mean fluorescence intensities of CD71 and CD36 in MDS patients compared with controls. These two parameters and the hemoglobin level were used to build a RED-score strongly suggestive of MDS if ≥ 3. Using the RED-score in the whole cohort, 80% of MDS or non-MDS patients were correctly classified. When combined with the flow score described by Ogata et al., this strategy allowed to reach a very high sensitivity of 88% of patients correctly classified.read more
Citations
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Journal ArticleDOI
Revisiting guidelines for integration of flow cytometry results in the WHO classification of myelodysplastic syndromes-proposal from the International/European LeukemiaNet Working Group for Flow Cytometry in MDS.
Anna Porwit,A.A. van de Loosdrecht,Peter Bettelheim,L Eidenschink Brodersen,Kate Burbury,Eline M. P. Cremers,M.G. Della Porta,Robin M. Ireland,Ulf Johansson,Sergio Matarraz,Kiyoyuki Ogata,A. Orfao,Frank Preijers,Katherina Psarra,Dolores Subirá,Peter Valent,V H J van der Velden,Denise A. Wells,Theresia M. Westers,Wolfgang Kern,M C Béné +20 more
TL;DR: In this paper, the authors provide guidelines that strongly recommend the integration of flow cytometry data with other diagnostic tools in the diagnostic work-up of myelodysplastic syndromes.
Journal ArticleDOI
Immunophenotypic analysis of erythroid dysplasia in myelodysplastic syndromes. A report from the IMDSFlow working group
Theresia M. Westers,Eline M. P. Cremers,Uta Oelschlaegel,Ulrika Johansson,Peter Bettelheim,Sergio Matarraz,Alberto Orfao,B. Moshaver,Lisa Eidenschink Brodersen,Michael R. Loken,Denise A. Wells,Dolores Subirá,Matthew J. Cullen,Jeroen G. te Marvelde,Vincent H.J. van der Velden,Frank Preijers,Sung-Chao Chu,Jean Feuillard,Estelle Guérin,Katherina Psarra,Anna Porwit,Leonie Saft,Robin M. Ireland,T. Milne,Marie C. Béné,Birgit I. Witte,Matteo G. Della Porta,Wolfgang Kern,Arjan A. van de Loosdrecht +28 more
TL;DR: A multicenter study within the IMDSFlow Working Group focused on defining flow cytometry parameters that enable discrimination of dyserythropoiesis associated with myelodysplastic syndromes from non-clonal cytopenias and found that analysis of expression of CD36 and CD71, in combination with CD71 fluorescence intensity and the percentage of CD117+ erythroid progenitors provided the best discrimination.
Journal ArticleDOI
Implementation of erythroid lineage analysis by flow cytometry in diagnostic models for myelodysplastic syndromes
Eline M. P. Cremers,Theresia M. Westers,Canan Alhan,Claudia Cali,Heleen Visser-Wisselaar,Dana A. Chitu,Vincent H.J. van der Velden,Jeroen G. te Marvelde,Saskia K. Klein,Petra Muus,Edo Vellenga,Georgina E. de Greef,Marie Cecile J.C. Legdeur,Pierre W. Wijermans,Marian Stevens-Kroef,Pedro da Silva-Coelho,Joop H. Jansen,Gert J. Ossenkoppele,Arjan A. van de Loosdrecht +18 more
TL;DR: This work validated the analysis of erythroid lineage parameters recommended by the International/European LeukemiaNet Working Group for Flow Cytometry in Myelodysplastic Syndromes, and incorporated this evaluation in currently applied flow cytometric models.
Journal ArticleDOI
Bone marrow immunophenotyping by flow cytometry in refractory cytopenia of childhood
Anna M. Aalbers,Marry M. van den Heuvel-Eibrink,Irith Baumann,Michael Dworzak,Henrik Hasle,Franco Locatelli,Barbara De Moerloose,Markus Schmugge,Ester Mejstrikova,Michaela Novakova,Marco Zecca,C. Michel Zwaan,Jeroen G. te Marvelde,Anton W. Langerak,Jacques J.M. van Dongen,Rob Pieters,Charlotte M. Niemeyer,Vincent H.J. van der Velden +17 more
TL;DR: It is concluded that flow cytometric immunophenotyping could be a relevant addition to histopathology in the diagnosis of refractory cytopenia of childhood.
Journal ArticleDOI
Multiparameter flow cytometry is instrumental to distinguish myelodysplastic syndromes from non-neoplastic cytopenias
Eline M. P. Cremers,Theresia M. Westers,Canan Alhan,Claudia Cali,Marielle J. Wondergem,Pino J. Poddighe,Gert J. Ossenkoppele,Arjan A. van de Loosdrecht +7 more
TL;DR: Flow cytometry (FC) can aid as a valuable tool to exclude MDS when CM and additional CG are not conclusive in patients with cytopenia.
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