Journal ArticleDOI
Fluorescent sterols as tools in membrane biophysics and cell biology.
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TLDR
This review provides an overview of various fluorescent sterols used in membrane biophysics and cell biology including analogs of cholesterol and cholesteryl esters, and attention is paid to the natural fluorescent sterol dehydroergosterol (DHE).About:
This article is published in Chemistry and Physics of Lipids.The article was published on 2007-03-01. It has received 142 citations till now. The article focuses on the topics: Intracellular sterol transport & Membrane permeability.read more
Citations
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Fluorescent Probes for Lipid Rafts: From Model Membranes to Living Cells
Andrey S. Klymchenko,Rémy Kreder +1 more
TL;DR: An urgent need to develop new probes, specifically adapted for cell plasma membranes and compatible with modern fluorescence microscopy techniques to push the understanding of membrane microdomains forward is highlighted.
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Partitioning, diffusion, and ligand binding of raft lipid analogs in model and cellular plasma membranes.
Erdinc Sezgin,Ilya Levental,Michał Grzybek,Günter Schwarzmann,Veronika Mueller,Alf Honigmann,Vladimir N. Belov,Christian Eggeling,Uenal Coskun,Kai Simons,Petra Schwille +10 more
TL;DR: Fluorescence correlation spectroscopy by stimulated emission depletion (STED) nanoscopy on intact cellular plasma membranes consistently reveals a constant level of confined diffusion for raft lipid analogs that vary greatly in their partitioning behavior, suggesting different physicochemical bases for these phenomena.
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Role of cholesterol in the function and organization of G-protein coupled receptors.
TL;DR: A novel mechanism by which membrane cholesterol could affect structure and function of GPCRs is proposed and it is envisaged that there could be specific/nonannular cholesterol binding site(s) in the serotonin(1A) receptor.
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BODIPY-Cholesterol: A New Tool to Visualize Sterol Trafficking in Living Cells and Organisms
Maarit Hölttä-Vuori,Riikka-Liisa Uronen,Jarmila Repáková,Emppu Salonen,Ilpo Vattulainen,Ilpo Vattulainen,Ilpo Vattulainen,Pertti Panula,Zaiguo Li,Robert Bittman,Elina Ikonen +10 more
TL;DR: This study employs atomistic simulations and experiments to characterize a cholesterol compound with fluorescent boron dipyrromethene difluoride linked to sterol carbon‐24 (BODIPY‐cholesterol), and concludes that this new probe closely mimics the membrane partitioning and trafficking of cholesterol and enables the direct monitoring of sterol movement by time‐lapse imaging using trace amounts of the probe.
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Intracellular sterol dynamics
TL;DR: Although still poorly characterized in many trafficking routes, several potential sterol transport proteins have been described in detail; as a result, molecular mechanisms for sterols transport among membranes start to be appreciated.
References
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Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
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Lipid sorting in epithelial cells.
Kai Simons,G. van Meer +1 more
TL;DR: Lipid biochemistry has remained a fairly esoteric branch of molecular cell biology, but this situation is now gradually changing with the discovery of phosphoinositide involvement in signal transduction.
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Use of resonance energy-transfer to monitor membrane-fusion
TL;DR: An assay for vesicle--vesicle fusion involving resonance energy transfer between N-(7-nitro-2,1,3-benzoxadiazol-4-yl), the energy donor, and rhodamine, the energy acceptor has been developed.
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Deuterium magnetic resonance: theory and application to lipid membranes.
TL;DR: Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton-proton and proton–carbon dipolar interactions and are rather difficult to analyse.
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Lipid asymmetry in membranes
TL;DR: Three sections of this chapter describing various approaches to study membrane lipid asymmetry are written by three different groups.